##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I AlwNI ApaLI AscI AseI
AsnI Asp718I BamHI BbeI BfrI
BsaWI BseAI BsiWI BspDI BspEI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoRI EcoT22I
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII ScaI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SspI SwaI Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2399 GACGTC GACGT^C
AccI 650 GTCGAC GT^[AC][GT]AC
AcsI 1793 GAATTT [GA]^AATT[CT]
AhdI 346 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2129 AGCGCT AGC^GCT
ApoI 1793 GAATTT [GA]^AATT[CT]
Asp700I 2695 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 346 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2843 CTCGGG C^[CT]CG[GA]G
AvrII 199 CCTAGG C^CTAGG
BbvCI 919 GCTGAGG CCTCAGC(-5/-2)
BcgI 642 GCACTCACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1155 GTATCC GTATCC(6/5)
BclI 1419 TGATCA T^GATCA
BlnI 199 CCTAGG C^CTAGG
BsaBI 2644 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1257 GAGACG CGTCTC(1/5)
BsmI 3354 GCATTC GAATGC(1/-1)
BsoBI 2843 CTCGGG C^[CT]CG[GA]G
Bsp1407I 2268 TGTACA T^GTACA
BspHI 1492 TCATGA T^CATGA
BspLU11I 693 ACATGT A^CATGT
BsrGI 2268 TGTACA T^GTACA
BssHII 2363 GCGCGC G^CGCGC
BstAPI 1017 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 964 GGTGACC G^GT[ACGT]ACC
BstPI 964 GGTGACC G^GT[ACGT]ACC
DraIII 569 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1101 CGGCCG C^GGCCG
Eam1105I 346 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1101 CGGCCG C^GGCCG
Eco47III 2129 AGCGCT AGC^GCT
Eco52I 1101 CGGCCG C^GGCCG
EcoO65I 964 GGTGACC G^GT[ACGT]ACC
EcoRV 1577 GATATC GAT^ATC
FbaI 1419 TGATCA T^GATCA
FseI 1102 GGCCGGCC GGCCGG^CC
MamI 2644 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
RcaI 1492 TCATGA T^CATGA
SalI 650 GTCGAC G^TCGAC
SgrAI 783 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1628 CTCAAG C^T[CT][GA]AG
SspBI 2268 TGTACA T^GTACA
StuI 1510 AGGCCT AGG^CCT
XmnI 2695 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 693 ACATGT A^C[GA][CT]GT
2154 ACACGT
AocI 776 CCTGAGG CC^T[ACGT]AGG
2278 CCTGAGG
AspI 2033 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2396 GACGACGTC
BanI 781 GGCACC G^G[CT][GA]CC
1716 GGCACC
BbrPI 755 CACGTG CAC^GTG
1358 CACGTG
BglI 296 GCCTATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3100 GCCCTCTTGGC
BlpI 994 GCTCAGC GC^T[ACGT]AGC
2135 GCTGAGC
Bpu1102I 994 GCTCAGC GC^T[ACGT]AGC
2135 GCTGAGC
BsaI 1130 GAGACC GGTCTC(1/5)
1599 GAGACC
BsiEI 853 CGACCG CG[GA][CT]^CG
1101 CGGCCG
Bsu36I 776 CCTGAGG CC^T[ACGT]AGG
2278 CCTGAGG
CelII 994 GCTCAGC GC^T[ACGT]AGC
2135 GCTGAGC
Eco81I 776 CCTGAGG CC^T[ACGT]AGG
2278 CCTGAGG
MseI 3378 TTAA T^TAA
3385 TTAA
NaeI 1103 GCCGGC GCC^GGC
1194 GCCGGC
NcoI 57 CCATGG C^CATGG
1839 CCATGG
NgoMIV 1103 GCCGGC G^CCGGC
1194 GCCGGC
PflFI 2033 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2396 GACGACGTC
PmaCI 755 CACGTG CAC^GTG
1358 CACGTG
PmlI 755 CACGTG CAC^GTG
1358 CACGTG
SacI 242 GAGCTC GAGCT^C
2681 GAGCTC
SapI 2527 GCTCTTC GCTCTTC(1/4)
2683 GCTCTTC
SbfI 1888 CCTGCAGG CCTGCA^GG
3189 CCTGCAGG
SphI 420 GCATGC GCATG^C
2469 GCATGC
Sse8387I 1888 CCTGCAGG CCTGCA^GG
3189 CCTGCAGG
Tru9I 3378 TTAA T^TAA
3385 TTAA
Tth111I 2033 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2396 GACGACGTC
XcmI 279 CCATCCCAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2337 CCATGATCCGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaII AviII
BalI BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII