##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       AclI        AcyI        AflIII      AgeI        
AhdI        Aor51HI     AscI        AspEI       AviII       
BbeI        BcnI        BlpI        Bpu1102I    BsaHI       
BsiEI       BsiWI       BsmBI       BspDI       BspHI       
BspLU11I    BsrFI       Bst1107I    BstBI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoRI       EcoRV       FseI        FspI        HgaI        
Hin1I       HpaI        KasI        KspI        MfeI        
MluI        MunI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    SwaI        XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I       1399  GGTACC               G^GTACC
AccII        2503  CGCG                 CG^CG
AccIII         71  TCCGGA               T^CCGGA
AflII         794  CTTAAG               C^TTAAG
Alw44I       2494  GTGCAC               G^TGCAC
AlwNI        1281  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        2494  GTGCAC               G^TGCAC
AseI         3410  ATTAAT               AT^TAAT
AsnI         3410  ATTAAT               AT^TAAT
Asp718I      1399  GGTACC               G^GTACC
AspI         2160  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1720  CCCGAG               C^[CT]CG[GA]G
AvrII        3952  CCTAGG               C^CTAGG
BamHI         167  GGATCC               G^GATCC
BanI         1399  GGTACC               G^G[CT][GA]CC
BbrPI        2497  CACGTG               CAC^GTG
BcgI         4517  CGAAGCTTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          794  CTTAAG               C^TTAAG
BglI         2352  GCCTTCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         3952  CCTAGG               C^CTAGG
BsaAI        2497  CACGTG               [CT]AC^GT[GA]
BsaBI        1179  GATACTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          656  GAGACC               GGTCTC(1/5)
BseAI          71  TCCGGA               T^CCGGA
BsoBI        1720  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     3474  TGTACA               T^GTACA
BspEI          71  TCCGGA               T^CCGGA
BsrBI        1395  GAGCGG               CCGCTC(-3/-3)
BsrGI        3474  TGTACA               T^GTACA
BssHII       2502  GCGCGC               G^CGCGC
BssSI         562  CACGAG               CACGAG(-5/-1)
BstAPI       3440  GCAGTATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        2503  CGCG                 CG^CG
BstXI        3849  CCACACCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII       2337  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3967  GACACCTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      4461  ATGCAT               ATGCA^T
HaeII        3640  AGCGCC               [GA]GCGC^[CT]
HindIII      4519  AAGCTT               A^AGCTT
KpnI         1399  GGTACC               GGTAC^C
MamI         1179  GATACTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI           71  TCCGGA               T^CCGGA
MvnI         2503  CGCG                 CG^CG
NcoI         2018  CCATGG               C^CATGG
NsiI         4461  ATGCAT               ATGCA^T
PflFI        2160  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2497  CACGTG               CAC^GTG
PmlI         2497  CACGTG               CAC^GTG
PshBI        3410  ATTAAT               AT^TAAT
SexAI        3596  ACCAGGT              A^CC[AT]GGT
SpeI         3216  ACTAGT               A^CTAGT
SspBI        3474  TGTACA               T^GTACA
StuI         4675  AGGCCT               AGG^CCT
Tth111I      2160  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          949  GTCTAC               GT^[AC][GT]AC
             1771  GTCTAC              
ApaI         3492  GGGCCC               GGGCC^C
             4495  GGGCCC              
Asp700I      1293  GAACCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2035  GAAAAGATTC          
BalI         2746  TGGCCA               TGG^CCA
             3709  TGGCCA              
BciVI        1178  GGATAC               GTATCC(6/5)
             1087  GTATCC              
BglII        1250  AGATCT               A^GATCT
             4311  AGATCT              
BpmI         1131  CTGGAG               CTGGAG(16/14)
             1272  CTGGAG              
BsaWI          71  TCCGGA               [AT]^CCGG[AT]
             1710  ACCGGA              
BsmI         2632  GAATGC               GAATGC(1/-1)
             1221  GCATTC              
BstEII       3039  GGTCACC              G^GT[ACGT]ACC
             4534  GGTAACC             
BstPI        3039  GGTCACC              G^GT[ACGT]ACC
             4534  GGTAACC             
DsaI         1755  CCGTGG               C^C[GA][CT]GG
             2018  CCATGG              
EcoO65I      3039  GGTCACC              G^GT[ACGT]ACC
             4534  GGTAACC             
FauI         2375  CCCGC                CCCGC(4/6)
             2470  CCCGC               
HapII          72  CCGG                 C^CGG
             1711  CCGG                
HincII        309  GTCAAC               GT[CT]^[GA]AC
             3106  GTTGAC              
HindII        309  GTCAAC               GT[CT]^[GA]AC
             3106  GTTGAC              
HpaII          72  CCGG                 C^CGG
             1711  CCGG                
MluNI        2746  TGGCCA               TGG^CCA
             3709  TGGCCA              
MscI         2746  TGGCCA               TGG^CCA
             3709  TGGCCA              
MspI           72  CCGG                 C^CGG
             1711  CCGG                
PstI          804  CTGCAG               CTGCA^G
             3917  CTGCAG              
XmnI         1293  GAACCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2035  GAAAAGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        AspHI       AvaII       BanII       
BbsI        BbvCI       BbvI        BclI        BfaI        
BmyI        BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     FbaI        Fnu4HI      FokI        
HaeIII      HhaI        HinP1I      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PvuII       RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII