################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AclI AcyI AflIII AgeI AhdI Aor51HI AscI AspEI AviII BbeI BcnI BlpI Bpu1102I BsaHI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BsrFI Bst1107I BstBI BstZ17I CelII Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FseI FspI HgaI Hin1I HpaI KasI KspI MfeI MluI MunI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 1399 GGTACC G^GTACC AccII 2503 CGCG CG^CG AccIII 71 TCCGGA T^CCGGA AflII 794 CTTAAG C^TTAAG Alw44I 2494 GTGCAC G^TGCAC AlwNI 1281 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 2494 GTGCAC G^TGCAC AseI 3410 ATTAAT AT^TAAT AsnI 3410 ATTAAT AT^TAAT Asp718I 1399 GGTACC G^GTACC AspI 2160 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1720 CCCGAG C^[CT]CG[GA]G AvrII 3952 CCTAGG C^CTAGG BamHI 167 GGATCC G^GATCC BanI 1399 GGTACC G^G[CT][GA]CC BbrPI 2497 CACGTG CAC^GTG BcgI 4517 CGAAGCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 794 CTTAAG C^TTAAG BglI 2352 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3952 CCTAGG C^CTAGG BsaAI 2497 CACGTG [CT]AC^GT[GA] BsaBI 1179 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 656 GAGACC GGTCTC(1/5) BseAI 71 TCCGGA T^CCGGA BsoBI 1720 CCCGAG C^[CT]CG[GA]G Bsp1407I 3474 TGTACA T^GTACA BspEI 71 TCCGGA T^CCGGA BsrBI 1395 GAGCGG CCGCTC(-3/-3) BsrGI 3474 TGTACA T^GTACA BssHII 2502 GCGCGC G^CGCGC BssSI 562 CACGAG CACGAG(-5/-1) BstAPI 3440 GCAGTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 2503 CGCG CG^CG BstXI 3849 CCACACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 2337 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3967 GACACCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 4461 ATGCAT ATGCA^T HaeII 3640 AGCGCC [GA]GCGC^[CT] HindIII 4519 AAGCTT A^AGCTT KpnI 1399 GGTACC GGTAC^C MamI 1179 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 71 TCCGGA T^CCGGA MvnI 2503 CGCG CG^CG NcoI 2018 CCATGG C^CATGG NsiI 4461 ATGCAT ATGCA^T PflFI 2160 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2497 CACGTG CAC^GTG PmlI 2497 CACGTG CAC^GTG PshBI 3410 ATTAAT AT^TAAT SexAI 3596 ACCAGGT A^CC[AT]GGT SpeI 3216 ACTAGT A^CTAGT SspBI 3474 TGTACA T^GTACA StuI 4675 AGGCCT AGG^CCT Tth111I 2160 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 949 GTCTAC GT^[AC][GT]AC 1771 GTCTAC ApaI 3492 GGGCCC GGGCC^C 4495 GGGCCC Asp700I 1293 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2035 GAAAAGATTC BalI 2746 TGGCCA TGG^CCA 3709 TGGCCA BciVI 1178 GGATAC GTATCC(6/5) 1087 GTATCC BglII 1250 AGATCT A^GATCT 4311 AGATCT BpmI 1131 CTGGAG CTGGAG(16/14) 1272 CTGGAG BsaWI 71 TCCGGA [AT]^CCGG[AT] 1710 ACCGGA BsmI 2632 GAATGC GAATGC(1/-1) 1221 GCATTC BstEII 3039 GGTCACC G^GT[ACGT]ACC 4534 GGTAACC BstPI 3039 GGTCACC G^GT[ACGT]ACC 4534 GGTAACC DsaI 1755 CCGTGG C^C[GA][CT]GG 2018 CCATGG EcoO65I 3039 GGTCACC G^GT[ACGT]ACC 4534 GGTAACC FauI 2375 CCCGC CCCGC(4/6) 2470 CCCGC HapII 72 CCGG C^CGG 1711 CCGG HincII 309 GTCAAC GT[CT]^[GA]AC 3106 GTTGAC HindII 309 GTCAAC GT[CT]^[GA]AC 3106 GTTGAC HpaII 72 CCGG C^CGG 1711 CCGG MluNI 2746 TGGCCA TGG^CCA 3709 TGGCCA MscI 2746 TGGCCA TGG^CCA 3709 TGGCCA MspI 72 CCGG C^CGG 1711 CCGG PstI 804 CTGCAG CTGCA^G 3917 CTGCAG XmnI 1293 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2035 GAAAAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AocI ApoI AspHI AvaII BanII BbsI BbvCI BbvI BclI BfaI BmyI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII