##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AclI        AflII       AscI        AviII       AvrII       
BbeI        BclI        BfrI        BglII       BlnI        
BsaBI       BsiWI       BsmI        BspDI       BspLU11I    
BssHII      BssSI       BstAPI      BstBI       ClaI        
CpoI        DraI        DrdI        EcoRV       EcoT22I     
FbaI        FseI        FspI        HpaI        KasI        
MamI        MfeI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PmeI        Psp1406I    PvuI        RsrII       SalI        
SbfI        SfiI        SfoI        SfuI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        1292  GACGTC               GACGT^C
Acc65I        980  GGTACC               G^GTACC
AccI         2784  GTATAC               GT^[AC][GT]AC
AccIII       1425  TCCGGA               T^CCGGA
AcyI         1292  GACGTC               G[GA]^CG[CT]C
AgeI         1040  ACCGGT               A^CCGGT
AhdI         2321  GACCAAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI       250  AGCGCT               AGC^GCT
AseI         1379  ATTAAT               AT^TAAT
AsnI         1379  ATTAAT               AT^TAAT
Asp718I       980  GGTACC               G^GTACC
AspEI        2321  GACCAAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1532  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        2635  CACGTG               CAC^GTG
BciVI        3434  GGATAC               GTATCC(6/5)
BsaHI        1292  GACGTC               G[GA]^CG[CT]C
BseAI        1425  TCCGGA               T^CCGGA
BspEI        1425  TCCGGA               T^CCGGA
BsrDI        1299  GCAATG               GCAATG(2/0)
Bst1107I     2784  GTATAC               GTA^TAC
BstEII       2674  GGTCACC              G^GT[ACGT]ACC
BstPI        2674  GGTCACC              G^GT[ACGT]ACC
BstZ17I      2784  GTATAC               GTA^TAC
DraIII       1403  CACGTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         3531  CGGCCG               C^GGCCG
Eam1105I     2321  GACCAAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3531  CGGCCG               C^GGCCG
Eco47III      250  AGCGCT               AGC^GCT
Eco52I       3531  CGGCCG               C^GGCCG
EcoO65I      2674  GGTCACC              G^GT[ACGT]ACC
HgaI         3798  GACGC                GACGC(5/10)
Hin1I        1292  GACGTC               G[GA]^CG[CT]C
HincII       2477  GTTGAC               GT[CT]^[GA]AC
HindII       2477  GTTGAC               GT[CT]^[GA]AC
KpnI          980  GGTACC               GGTAC^C
KspI          365  CCGCGG               CCGC^GG
MroI         1425  TCCGGA               T^CCGGA
NcoI           63  CCATGG               C^CATGG
NspI         1752  GCATGT               [GA]CATG^[CT]
PflFI        1532  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1040  ACCGGT               A^CCGGT
PmaCI        2635  CACGTG               CAC^GTG
PmlI         2635  CACGTG               CAC^GTG
PshAI         781  GACCTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1379  ATTAAT               AT^TAAT
SacII         365  CCGCGG               CCGC^GG
SexAI        3738  ACCTGGT              A^CC[AT]GGT
SgrAI        1039  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         4028  CCCGGG               CCC^GGG
SspI          305  AATATT               AAT^ATT
StuI         1966  AGGCCT               AGG^CCT
Tth111I      1532  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1990  TCTAGA               T^CTAGA
XmaCI        4028  CCCGGG               C^CCGGG
XmaI         4028  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AcsI         2272  GAATTC               [GA]^AATT[CT]
             4247  GAATTC              
Alw44I       1559  GTGCAC               G^TGCAC
             3591  GTGCAC              
AocI          408  CCTCAGG              CC^T[ACGT]AGG
             2627  CCTGAGG             
ApaLI        1559  GTGCAC               G^TGCAC
             3591  GTGCAC              
ApoI         2272  GAATTC               [GA]^AATT[CT]
             4247  GAATTC              
Asp700I      2105  GAACTCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3685  GAACCCCTTC          
AvaI         1413  CCCGAG               C^[CT]CG[GA]G
             4028  CCCGGG              
BbsI          260  GAAGAC               GAAGAC(2/6)
             4202  GAAGAC              
BcgI         3583  CGAGTACTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1964  GCAGGCCTCTCG        
BglI         2910  GCCTGCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3072  GCCCGCTGGGC         
BlpI          167  GCTGAGC              GC^T[ACGT]AGC
             2939  GCTGAGC             
Bpu1102I      167  GCTGAGC              GC^T[ACGT]AGC
             2939  GCTGAGC             
BpuAI         260  GAAGAC               GAAGAC(2/6)
             4202  GAAGAC              
BsaAI         851  TACGTG               [CT]AC^GT[GA]
             2635  CACGTG              
BsmBI        2766  GAGACG               CGTCTC(1/5)
             3796  GAGACG              
BsoBI        1413  CCCGAG               C^[CT]CG[GA]G
             4028  CCCGGG              
Bsp1407I      311  TGTACA               T^GTACA
             3663  TGTACA              
BspHI        3756  TCATGA               T^CATGA
             4260  TCATGA              
BsrFI        1040  ACCGGT               [GA]^CCGG[CT]
             2465  ACCGGC              
BsrGI         311  TGTACA               T^GTACA
             3663  TGTACA              
Bsu36I        408  CCTCAGG              CC^T[ACGT]AGG
             2627  CCTGAGG             
CelII         167  GCTGAGC              GC^T[ACGT]AGC
             2939  GCTGAGC             
Cfr10I       1040  ACCGGT               [GA]^CCGG[CT]
             2465  ACCGGC              
Eco81I        408  CCTCAGG              CC^T[ACGT]AGG
             2627  CCTGAGG             
EcoNI         607  CCTCAGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1517  CCTGCTCCAGG         
EcoRI        2272  GAATTC               G^AATTC
             4247  GAATTC              
HindIII        36  AAGCTT               A^AGCTT
              513  AAGCTT              
PflMI        2979  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3896  CCAGAAAGTGG         
RcaI         3756  TCATGA               T^CATGA
             4260  TCATGA              
SapI          966  GAAGAGC              GCTCTTC(1/4)
             2027  GAAGAGC             
ScaI         3045  AGTACT               AGT^ACT
             3585  AGTACT              
SspBI         311  TGTACA               T^GTACA
             3663  TGTACA              
Van91I       2979  CCAGACCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3896  CCAGAAAGTGG         
XmnI         2105  GAACTCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3685  GAACCCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApaI        AspHI       AvaII       
BalI        BamHI       BanI        BanII       BbvCI       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrBI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII