################################## # NO CUT ENZYMES ( 60 enzymes) # ################################## AclI AflII AscI AviII AvrII BbeI BclI BfrI BglII BlnI BsaBI BsiWI BsmI BspDI BspLU11I BssHII BssSI BstAPI BstBI ClaI CpoI DraI DrdI EcoRV EcoT22I FbaI FseI FspI HpaI KasI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PmeI Psp1406I PvuI RsrII SalI SbfI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 1292 GACGTC GACGT^C Acc65I 980 GGTACC G^GTACC AccI 2784 GTATAC GT^[AC][GT]AC AccIII 1425 TCCGGA T^CCGGA AcyI 1292 GACGTC G[GA]^CG[CT]C AgeI 1040 ACCGGT A^CCGGT AhdI 2321 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 250 AGCGCT AGC^GCT AseI 1379 ATTAAT AT^TAAT AsnI 1379 ATTAAT AT^TAAT Asp718I 980 GGTACC G^GTACC AspEI 2321 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1532 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 2635 CACGTG CAC^GTG BciVI 3434 GGATAC GTATCC(6/5) BsaHI 1292 GACGTC G[GA]^CG[CT]C BseAI 1425 TCCGGA T^CCGGA BspEI 1425 TCCGGA T^CCGGA BsrDI 1299 GCAATG GCAATG(2/0) Bst1107I 2784 GTATAC GTA^TAC BstEII 2674 GGTCACC G^GT[ACGT]ACC BstPI 2674 GGTCACC G^GT[ACGT]ACC BstZ17I 2784 GTATAC GTA^TAC DraIII 1403 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 3531 CGGCCG C^GGCCG Eam1105I 2321 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3531 CGGCCG C^GGCCG Eco47III 250 AGCGCT AGC^GCT Eco52I 3531 CGGCCG C^GGCCG EcoO65I 2674 GGTCACC G^GT[ACGT]ACC HgaI 3798 GACGC GACGC(5/10) Hin1I 1292 GACGTC G[GA]^CG[CT]C HincII 2477 GTTGAC GT[CT]^[GA]AC HindII 2477 GTTGAC GT[CT]^[GA]AC KpnI 980 GGTACC GGTAC^C KspI 365 CCGCGG CCGC^GG MroI 1425 TCCGGA T^CCGGA NcoI 63 CCATGG C^CATGG NspI 1752 GCATGT [GA]CATG^[CT] PflFI 1532 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1040 ACCGGT A^CCGGT PmaCI 2635 CACGTG CAC^GTG PmlI 2635 CACGTG CAC^GTG PshAI 781 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1379 ATTAAT AT^TAAT SacII 365 CCGCGG CCGC^GG SexAI 3738 ACCTGGT A^CC[AT]GGT SgrAI 1039 CACCGGTG C[GA]^CCGG[CT]G SmaI 4028 CCCGGG CCC^GGG SspI 305 AATATT AAT^ATT StuI 1966 AGGCCT AGG^CCT Tth111I 1532 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1990 TCTAGA T^CTAGA XmaCI 4028 CCCGGG C^CCGGG XmaI 4028 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcsI 2272 GAATTC [GA]^AATT[CT] 4247 GAATTC Alw44I 1559 GTGCAC G^TGCAC 3591 GTGCAC AocI 408 CCTCAGG CC^T[ACGT]AGG 2627 CCTGAGG ApaLI 1559 GTGCAC G^TGCAC 3591 GTGCAC ApoI 2272 GAATTC [GA]^AATT[CT] 4247 GAATTC Asp700I 2105 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3685 GAACCCCTTC AvaI 1413 CCCGAG C^[CT]CG[GA]G 4028 CCCGGG BbsI 260 GAAGAC GAAGAC(2/6) 4202 GAAGAC BcgI 3583 CGAGTACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1964 GCAGGCCTCTCG BglI 2910 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3072 GCCCGCTGGGC BlpI 167 GCTGAGC GC^T[ACGT]AGC 2939 GCTGAGC Bpu1102I 167 GCTGAGC GC^T[ACGT]AGC 2939 GCTGAGC BpuAI 260 GAAGAC GAAGAC(2/6) 4202 GAAGAC BsaAI 851 TACGTG [CT]AC^GT[GA] 2635 CACGTG BsmBI 2766 GAGACG CGTCTC(1/5) 3796 GAGACG BsoBI 1413 CCCGAG C^[CT]CG[GA]G 4028 CCCGGG Bsp1407I 311 TGTACA T^GTACA 3663 TGTACA BspHI 3756 TCATGA T^CATGA 4260 TCATGA BsrFI 1040 ACCGGT [GA]^CCGG[CT] 2465 ACCGGC BsrGI 311 TGTACA T^GTACA 3663 TGTACA Bsu36I 408 CCTCAGG CC^T[ACGT]AGG 2627 CCTGAGG CelII 167 GCTGAGC GC^T[ACGT]AGC 2939 GCTGAGC Cfr10I 1040 ACCGGT [GA]^CCGG[CT] 2465 ACCGGC Eco81I 408 CCTCAGG CC^T[ACGT]AGG 2627 CCTGAGG EcoNI 607 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1517 CCTGCTCCAGG EcoRI 2272 GAATTC G^AATTC 4247 GAATTC HindIII 36 AAGCTT A^AGCTT 513 AAGCTT PflMI 2979 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3896 CCAGAAAGTGG RcaI 3756 TCATGA T^CATGA 4260 TCATGA SapI 966 GAAGAGC GCTCTTC(1/4) 2027 GAAGAGC ScaI 3045 AGTACT AGT^ACT 3585 AGTACT SspBI 311 TGTACA T^GTACA 3663 TGTACA Van91I 2979 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3896 CCAGAAAGTGG XmnI 2105 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3685 GAACCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaII BalI BamHI BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII