##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AccIII      AclI        AgeI        AhdI        AscI        
AseI        AsnI        AspEI       AspI        BbeI        
BbrPI       BcgI        BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BsrFI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoRI       EcoRV       EcoT22I     
FseI        HindIII     KasI        KspI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII        1085  GACGTC               GACGT^C
Acc65I       1986  GGTACC               G^GTACC
AccI         1397  GTAGAC               GT^[AC][GT]AC
AcsI         2999  AAATTC               [GA]^AATT[CT]
AflII        3572  CTTAAG               C^TTAAG
Alw44I       1733  GTGCAC               G^TGCAC
ApaLI        1733  GTGCAC               G^TGCAC
ApoI         2999  AAATTC               [GA]^AATT[CT]
Asp700I      2159  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1986  GGTACC               G^GTACC
AvrII         548  CCTAGG               C^CTAGG
BamHI         771  GGATCC               G^GATCC
BanI         1986  GGTACC               G^G[CT][GA]CC
BciVI        2394  GTATCC               GTATCC(6/5)
BfrI         3572  CTTAAG               C^TTAAG
BglI         1553  GCCTGGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1878  AGATCT               A^GATCT
BlnI          548  CCTAGG               C^CTAGG
BsgI         2189  GTGCAG               GTGCAG(16/14)
BsmI         2163  GCATTC               GAATGC(1/-1)
Bsp1407I      864  TGTACA               T^GTACA
BspHI        2172  TCATGA               T^CATGA
BspLU11I     1566  ACATGT               A^CATGT
BsrBI        2666  GAGCGG               CCGCTC(-3/-3)
BsrDI        3231  GCAATG               GCAATG(2/0)
BsrGI         864  TGTACA               T^GTACA
BssSI        1436  CACGAG               CACGAG(-5/-1)
HincII        415  GTTAAC               GT[CT]^[GA]AC
HindII        415  GTTAAC               GT[CT]^[GA]AC
HpaI          415  GTTAAC               GTT^AAC
KpnI         1986  GGTACC               GGTAC^C
NruI          922  TCGCGA               TCG^CGA
RcaI         2172  TCATGA               T^CATGA
SacI         2525  GAGCTC               GAGCT^C
SbfI         1021  CCTGCAGG             CCTGCA^GG
ScaI         2232  AGTACT               AGT^ACT
SmaI         1473  CCCGGG               CCC^GGG
SphI          781  GCATGC               GCATG^C
Sse8387I     1021  CCTGCAGG             CCTGCA^GG
SspBI         864  TGTACA               T^GTACA
SspI         3729  AATATT               AAT^ATT
StuI          163  AGGCCT               AGG^CCT
XmaCI        1473  CCCGGG               C^CCGGG
XmaI         1473  CCCGGG               C^CCGGG
XmnI         2159  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII          24  CGCG                 CG^CG
              923  CGCG                
AcyI           43  GGCGTC               G[GA]^CG[CT]C
             1085  GACGTC              
AviII        2781  TGCGCA               TGC^GCA
             3525  TGCGCA              
BsaHI          43  GGCGTC               G[GA]^CG[CT]C
             1085  GACGTC              
BstUI          24  CGCG                 CG^CG
              923  CGCG                
EarI         1169  CTCTTC               CTCTTC(1/4)
              122  GAAGAG              
FauI         1344  CCCGC                CCCGC(4/6)
             2668  GCGGG               
FspI         2781  TGCGCA               TGC^GCA
             3525  TGCGCA              
HgaI           44  GCGTC                GACGC(5/10)
             2584  GCGTC               
Hin1I          43  GGCGTC               G[GA]^CG[CT]C
             1085  GACGTC              
Ksp632I      1169  CTCTTC               CTCTTC(1/4)
              122  GAAGAG              
MvnI           24  CGCG                 CG^CG
              923  CGCG                
PflMI        2959  CCAACATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3208  CCACTTCTTGG         
PpuMI         430  GGGACCC              [GA]G^G[AT]CC[CT]
             1320  GGGACCC             
PshAI         485  GACCGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1322  GACCCCAGTC          
PstI          951  CTGCAG               CTGCA^G
             1022  CTGCAG              
TaqI         2431  TCGA                 T^CGA
             2587  TCGA                
TthHB8I      2431  TCGA                 T^CGA
             2587  TCGA                
Van91I       2959  CCAACATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3208  CCACTTCTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AfaI        AflIII      AluI        AlwI        
AlwNI       AocI        Aor51HI     ApaI        AspHI       
AvaI        AvaII       BalI        BanII       BbsI        
BbvCI       BbvI        BclI        BcnI        BfaI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstXI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        Eco47III    
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRII      
EcoT14I     FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XcmI        XhoII