##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AclI AflII AhdI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BciVI BfrI BlnI
BsaBI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HpaI KspI
MamI MfeI MluI MluNI MscI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PmeI
PshBI Psp1406I RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 779 GACGTC GACGT^C
Acc65I 3116 GGTACC G^GTACC
AccI 2102 GTCTAC GT^[AC][GT]AC
AcsI 1448 AAATTC [GA]^AATT[CT]
AflIII 2578 ACACGT A^C[GA][CT]GT
AgeI 1984 ACCGGT A^CCGGT
AocI 2516 CCTGAGG CC^T[ACGT]AGG
Aor51HI 204 AGCGCT AGC^GCT
ApoI 1448 AAATTC [GA]^AATT[CT]
Asp718I 3116 GGTACC G^GTACC
BamHI 925 GGATCC G^GATCC
BclI 3093 TGATCA T^GATCA
BglII 1029 AGATCT A^GATCT
BsmBI 41 GAGACG CGTCTC(1/5)
BsrDI 391 CATTGC GCAATG(2/0)
BssHII 713 GCGCGC G^CGCGC
Bsu36I 2516 CCTGAGG CC^T[ACGT]AGG
DraIII 2944 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 852 CGGCCG C^GGCCG
EclXI 852 CGGCCG C^GGCCG
Eco47III 204 AGCGCT AGC^GCT
Eco52I 852 CGGCCG C^GGCCG
Eco81I 2516 CCTGAGG CC^T[ACGT]AGG
FbaI 3093 TGATCA T^GATCA
HincII 746 GTCAAC GT[CT]^[GA]AC
HindII 746 GTCAAC GT[CT]^[GA]AC
HindIII 253 AAGCTT A^AGCTT
KpnI 3116 GGTACC GGTAC^C
MseI 2625 TTAA T^TAA
NaeI 1257 GCCGGC GCC^GGC
NcoI 2523 CCATGG C^CATGG
NgoMIV 1257 GCCGGC G^CCGGC
PflMI 1702 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1984 ACCGGT A^CCGGT
PvuI 2062 CGATCG CGAT^CG
SgrAI 1866 CGCCGGTG C[GA]^CCGG[CT]G
Tru9I 2625 TTAA T^TAA
Tsp509I 1449 AATT ^AATT
Van91I 1702 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Alw44I 703 GTGCAC G^TGCAC
2973 GTGCAC
ApaLI 703 GTGCAC G^TGCAC
2973 GTGCAC
BbeI 150 GGCGCC GGCGC^C
408 GGCGCC
BbrPI 1835 CACGTG CAC^GTG
2579 CACGTG
BcgI 955 CGAGGTGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2056 GCAGCCCGATCG
BfaI 741 CTAG C^TAG
2804 CTAG
BsaAI 1835 CACGTG [CT]AC^GT[GA]
2579 CACGTG
BsiEI 852 CGGCCG CG[GA][CT]^CG
2062 CGATCG
BsmI 526 GAATGC GAATGC(1/-1)
1569 GCATTC
BstAPI 439 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1150 GCAGGCCCTGC
DsaI 217 CCGTGG C^C[GA][CT]GG
2523 CCATGG
KasI 150 GGCGCC G^GCGCC
408 GGCGCC
MaeI 741 CTAG C^TAG
2804 CTAG
NarI 150 GGCGCC GG^CGCC
408 GGCGCC
NspI 961 GCATGC [GA]CATG^[CT]
2358 GCATGC
PaeR7I 953 CTCGAG C^TCGAG
2326 CTCGAG
PmaCI 1835 CACGTG CAC^GTG
2579 CACGTG
PmlI 1835 CACGTG CAC^GTG
2579 CACGTG
PshAI 43 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2644 GACCTCTGTC
SapI 421 GAAGAGC GCTCTTC(1/4)
1456 GAAGAGC
SfoI 150 GGCGCC GGC^GCC
408 GGCGCC
SmaI 180 CCCGGG CCC^GGG
2754 CCCGGG
SphI 961 GCATGC GCATG^C
2358 GCATGC
XhoI 953 CTCGAG C^TCGAG
2326 CTCGAG
XmaCI 180 CCCGGG C^CCGGG
2754 CCCGGG
XmaI 180 CCCGGG C^CCGGG
2754 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MroI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XcmI XhoII