##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AflIII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
BbeI BbrPI BbvCI BcgI BciVI
BglI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BssHII BssSI BstAPI
BstBI BstEII BstPI ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FseI HaeII HpaI KasI KspI
MfeI MluI MroI MunI NarI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1865 GGTACC G^GTACC
AccII 1156 CGCG CG^CG
AclI 2744 AACGTT AA^CGTT
AcyI 935 GACGCC G[GA]^CG[CT]C
AflII 2884 CTTAAG C^TTAAG
Alw44I 3183 GTGCAC G^TGCAC
AlwNI 3572 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2246 CCTAAGG CC^T[ACGT]AGG
ApaLI 3183 GTGCAC G^TGCAC
Asp700I 771 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1865 GGTACC G^GTACC
AspI 1190 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2264 TGCGCA TGC^GCA
BamHI 1008 GGATCC G^GATCC
BcnI 1603 CCGGG CC^[GC]GG
BfrI 2884 CTTAAG C^TTAAG
BsaAI 2053 TACGTG [CT]AC^GT[GA]
BsaHI 935 GACGCC G[GA]^CG[CT]C
BsaWI 1584 ACCGGA [AT]^CCGG[AT]
Bsp1407I 3478 TGTACA T^GTACA
BspHI 1950 TCATGA T^CATGA
BsrBI 546 CCGCTC CCGCTC(-3/-3)
BsrGI 3478 TGTACA T^GTACA
BstUI 1156 CGCG CG^CG
Bsu36I 2246 CCTAAGG CC^T[ACGT]AGG
Eco81I 2246 CCTAAGG CC^T[ACGT]AGG
EcoRI 2660 GAATTC G^AATTC
FspI 2264 TGCGCA TGC^GCA
Hin1I 935 GACGCC G[GA]^CG[CT]C
HindIII 1294 AAGCTT A^AGCTT
KpnI 1865 GGTACC GGTAC^C
MvnI 1156 CGCG CG^CG
NciI 1603 CCGGG CC^[GC]GG
NcoI 2801 CCATGG C^CATGG
NdeI 4214 CATATG CA^TATG
PflFI 1190 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2006 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2744 AACGTT AA^CGTT
RcaI 1950 TCATGA T^CATGA
SacI 1928 GAGCTC GAGCT^C
ScaI 2968 AGTACT AGT^ACT
SpeI 208 ACTAGT A^CTAGT
SphI 2467 GCATGC GCATG^C
SspBI 3478 TGTACA T^GTACA
Tth111I 1190 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2006 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 771 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3846 GTATAC GT^[AC][GT]AC
4346 GTATAC
ApaI 1718 GGGCCC GGGCC^C
3656 GGGCCC
AvrII 2694 CCTAGG C^CTAGG
3903 CCTAGG
BclI 681 TGATCA T^GATCA
2543 TGATCA
BglII 1046 AGATCT A^GATCT
1467 AGATCT
BlnI 2694 CCTAGG C^CTAGG
3903 CCTAGG
BlpI 3301 GCTCAGC GC^T[ACGT]AGC
3618 GCTTAGC
Bpu1102I 3301 GCTCAGC GC^T[ACGT]AGC
3618 GCTTAGC
BsaI 1628 GAGACC GGTCTC(1/5)
4694 GAGACC
BsmBI 933 GAGACG CGTCTC(1/5)
3707 GAGACG
BsmI 2083 GAATGC GAATGC(1/-1)
3518 GCATTC
BsrDI 3759 CATTGC GCAATG(2/0)
1764 GCAATG
Bst1107I 3846 GTATAC GTA^TAC
4346 GTATAC
BstZ17I 3846 GTATAC GTA^TAC
4346 GTATAC
CelII 3301 GCTCAGC GC^T[ACGT]AGC
3618 GCTTAGC
DraI 2388 TTTAAA TTT^AAA
4650 TTTAAA
DsaI 87 CCGTGG C^C[GA][CT]GG
2801 CCATGG
EcoT22I 199 ATGCAT ATGCA^T
2452 ATGCAT
FbaI 681 TGATCA T^GATCA
2543 TGATCA
HgaI 935 GACGC GACGC(5/10)
3709 GACGC
NaeI 1653 GCCGGC GCC^GGC
1883 GCCGGC
NgoMIV 1653 GCCGGC G^CCGGC
1883 GCCGGC
NsiI 199 ATGCAT ATGCA^T
2452 ATGCAT
SspI 4472 AATATT AAT^ATT
4568 AATATT
StuI 1219 AGGCCT AGG^CCT
2761 AGGCCT
XbaI 277 TCTAGA T^CTAGA
3897 TCTAGA
XcmI 1770 CCAGTGGAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2145 CCACCACTGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NheI NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII