##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BbrPI BciVI BclI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BseRI
BsiWI BsmBI BsoBI BspDI BspEI
BspHI BsrBI BssHII BstEII BstPI
BstUI CelII ClaI CpoI DraIII
Eam1105I Eco47III EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HpaI
KpnI KspI MamI MluI MroI
MvnI NaeI NciI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AcyI 3006 GGCGCC G[GA]^CG[CT]C
AocI 1327 CCTCAGG CC^T[ACGT]AGG
AspHI 2666 GAGCTC G[AT]GC[AT]^C
BalI 1079 TGGCCA TGG^CCA
BamHI 2082 GGATCC G^GATCC
BanI 3006 GGCGCC G^G[CT][GA]CC
BbeI 3006 GGCGCC GGCGC^C
BbsI 274 GAAGAC GAAGAC(2/6)
BbvCI 500 CCTCAGC CCTCAGC(-5/-2)
BcgI 1053 GCAAGTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 274 GAAGAC GAAGAC(2/6)
BsaAI 1733 TACGTG [CT]AC^GT[GA]
BsaHI 3006 GGCGCC G[GA]^CG[CT]C
BsgI 491 CTGCAC GTGCAG(16/14)
BsiEI 2263 CGGCCG CG[GA][CT]^CG
BsiHKAI 2666 GAGCTC G[AT]GC[AT]^C
BsmFI 2197 GTCCC GGGAC(10/14)
Bsp1407I 340 TGTACA T^GTACA
BsrFI 2265 GCCGGT [GA]^CCGG[CT]
BsrGI 340 TGTACA T^GTACA
BssSI 392 CACGAG CACGAG(-5/-1)
BstBI 214 TTCGAA TT^CGAA
BstXI 533 CCATTGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2082 GGATCC [GA]^GATC[CT]
Bsu36I 1327 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2265 GCCGGT [GA]^CCGG[CT]
DraII 117 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 2263 CGGCCG C^GGCCG
EclXI 2263 CGGCCG C^GGCCG
Eco52I 2263 CGGCCG C^GGCCG
Eco81I 1327 CCTCAGG CC^T[ACGT]AGG
EcoO109I 117 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1377 CCATGG C^C[AT][AT]GG
HaeII 3006 GGCGCC [GA]GCGC^[CT]
HapII 2266 CCGG C^CGG
HgaI 19 GACGC GACGC(5/10)
Hin1I 3006 GGCGCC G[GA]^CG[CT]C
HindIII 2405 AAGCTT A^AGCTT
HpaII 2266 CCGG C^CGG
KasI 3006 GGCGCC G^GCGCC
MflI 2082 GGATCC [GA]^GATC[CT]
MluNI 1079 TGGCCA TGG^CCA
MscI 1079 TGGCCA TGG^CCA
MspI 2266 CCGG C^CGG
NarI 3006 GGCGCC GG^CGCC
NcoI 1377 CCATGG C^CATGG
NspV 214 TTCGAA TT^CGAA
PshAI 2968 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2666 GAGCTC GAGCT^C
ScaI 2103 AGTACT AGT^ACT
SexAI 1977 ACCAGGT A^CC[AT]GGT
SfoI 3006 GGCGCC GGC^GCC
SfuI 214 TTCGAA TT^CGAA
SspBI 340 TGTACA T^GTACA
SspI 567 AATATT AAT^ATT
StuI 1442 AGGCCT AGG^CCT
StyI 1377 CCATGG C^C[AT][AT]GG
SwaI 3257 ATTTAAAT ATTT^AAAT
Tsp45I 1019 GTGAC ^GT[GC]AC
XhoII 2082 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2466 GTATAC GT^[AC][GT]AC
3335 GTATAC
AclI 149 AACGTT AA^CGTT
2315 AACGTT
AlwNI 125 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2147 CAGAAGCTG
AseI 664 ATTAAT AT^TAAT
1747 ATTAAT
AsnI 664 ATTAAT AT^TAAT
1747 ATTAAT
AvaII 1120 GGACC G^G[AT]CC
2801 GGACC
BpmI 122 CTCCAG CTGGAG(16/14)
2937 CTGGAG
BsaI 1088 GAGACC GGTCTC(1/5)
1459 GAGACC
BsmAI 1088 GAGAC GTCTC(1/5)
1459 GAGAC
BsmI 22 GCATTC GAATGC(1/-1)
2363 GCATTC
BspMI 1069 GCAGGT ACCTGC(4/8)
2190 GCAGGT
Bst1107I 2466 GTATAC GTA^TAC
3335 GTATAC
BstAPI 355 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2489 GCACAGATTGC
BstZ17I 2466 GTATAC GTA^TAC
3335 GTATAC
DrdI 2802 GACCACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3027 GACAAATGTGTC
DsaI 1377 CCATGG C^C[GA][CT]GG
1454 CCACGG
EaeI 1079 TGGCCA [CT]^GGCC[GA]
2263 CGGCCG
EcoRI 1830 GAATTC G^AATTC
2761 GAATTC
HincII 463 GTTGAC GT[CT]^[GA]AC
3196 GTTGAC
HindII 463 GTTGAC GT[CT]^[GA]AC
3196 GTTGAC
MfeI 1292 CAATTG C^AATTG
1649 CAATTG
MslI 1375 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1563 CATTCAGATG
MspA1I 503 CAGCGG C[AC]G^C[GT]G
1676 CCGCTG
MunI 1292 CAATTG C^AATTG
1649 CAATTG
PflMI 391 CCACGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1645 CCAACAATTGG
PshBI 664 ATTAAT AT^TAAT
1747 ATTAAT
Psp1406I 149 AACGTT AA^CGTT
2315 AACGTT
Van91I 391 CCACGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1645 CCAACAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI ApoI Asp700I BanII
BbvI BfaI BfrI BmyI BsaJI
BsiYI BslI Bsp1286I BspLU11I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XmnI