##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AclI        AflIII      AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       AvrII       BalI        BamHI       BbrPI       
BbvCI       BciVI       BclI        BglI        BlnI        
BsaAI       BsaBI       BsaI        BsiEI       BsiWI       
BspDI       BspLU11I    BsrDI       BsrFI       BssHII      
BssSI       BstAPI      BstBI       Bsu36I      Cfr10I      
ClaI        CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MscI        MunI        NaeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        Van91I      XbaI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AccIII       1994  TCCGGA               T^CCGGA
AflII        1610  CTTAAG               C^TTAAG
AlwNI        1189  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1987  AGCGCT               AGC^GCT
AspHI        2432  GTGCTC               G[AT]GC[AT]^C
BbeI          142  GGCGCC               GGCGC^C
BfaI         2472  CTAG                 C^TAG
BfrI         1610  CTTAAG               C^TTAAG
BsaWI        1994  TCCGGA               [AT]^CCGG[AT]
BseAI        1994  TCCGGA               T^CCGGA
BsiHKAI      2432  GTGCTC               G[AT]GC[AT]^C
BspEI        1994  TCCGGA               T^CCGGA
BspHI        1177  TCATGA               T^CATGA
BspMI        2097  ACCTGC               ACCTGC(4/8)
BsrBI         206  GAGCGG               CCGCTC(-3/-3)
Bst1107I      545  GTATAC               GTA^TAC
BstEII       2463  GGTGACC              G^GT[ACGT]ACC
BstPI        2463  GGTGACC              G^GT[ACGT]ACC
BstZ17I       545  GTATAC               GTA^TAC
DrdI         1568  GACACTCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          975  TGGCCG               [CT]^GGCC[GA]
Eco47III     1987  AGCGCT               AGC^GCT
EcoNI         282  CCTCCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2463  GGTGACC              G^GT[ACGT]ACC
KasI          142  GGCGCC               G^GCGCC
MaeI         2472  CTAG                 C^TAG
MaeII        2363  ACGT                 A^CGT
MroI         1994  TCCGGA               T^CCGGA
MspA1I        215  CAGCGG               C[AC]G^C[GT]G
NarI          142  GGCGCC               GG^CGCC
NcoI         2328  CCATGG               C^CATGG
NspI         1968  GCATGT               [GA]CATG^[CT]
RcaI         1177  TCATGA               T^CATGA
SfoI          142  GGCGCC               GGC^GCC
StuI         1361  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AccI          545  GTATAC               GT^[AC][GT]AC
             1037  GTAGAC              
AccII          75  CGCG                 CG^CG
              521  CGCG                
AcyI            6  GACGCC               G[GA]^CG[CT]C
              142  GGCGCC              
AspI         1321  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1498  GACCAAGTC           
BanII        1202  GAGCCC               G[GA]GC[CT]^C
             2084  GGGCTC              
BcgI          123  CGATGCCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2091  CGAAGGACCTGC        
BglII         507  AGATCT               A^GATCT
              960  AGATCT              
BlpI         1886  GCTGAGC              GC^T[ACGT]AGC
             2077  GCTTAGC             
Bpu1102I     1886  GCTGAGC              GC^T[ACGT]AGC
             2077  GCTTAGC             
BsaHI           6  GACGCC               G[GA]^CG[CT]C
              142  GGCGCC              
BseRI          97  CTCCTC               GAGGAG(10/8)
             2104  GAGGAG              
BsgI          781  GTGCAG               GTGCAG(16/14)
              945  GTGCAG              
BsmBI           4  GAGACG               CGTCTC(1/5)
               71  GAGACG              
BsmI          397  GAATGC               GAATGC(1/-1)
             2696  GAATGC              
Bsp1407I     2654  TGTACA               T^GTACA
             2674  TGTACA              
BsrGI        2654  TGTACA               T^GTACA
             2674  TGTACA              
BstUI          75  CGCG                 CG^CG
              521  CGCG                
BstXI        1935  CCAAAAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2139  CCATACCTCTGG        
BstYI         507  AGATCT               [GA]^GATC[CT]
              960  AGATCT              
CelII        1886  GCTGAGC              GC^T[ACGT]AGC
             2077  GCTTAGC             
DraI          470  TTTAAA               TTT^AAA
              922  TTTAAA              
DsaI          870  CCACGG               C^C[GA][CT]GG
             2328  CCATGG              
FauI          482  GCGGG                CCCGC(4/6)
             2082  GCGGG               
Hin1I           6  GACGCC               G[GA]^CG[CT]C
              142  GGCGCC              
HincII       1085  GTTGAC               GT[CT]^[GA]AC
             1565  GTTGAC              
HindII       1085  GTTGAC               GT[CT]^[GA]AC
             1565  GTTGAC              
MflI          507  AGATCT               [GA]^GATC[CT]
              960  AGATCT              
MslI          536  CATGCCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1324  CATGTCGATG          
MvnI           75  CGCG                 CG^CG
              521  CGCG                
NdeI         1863  CATATG               CA^TATG
             2670  CATATG              
PaeR7I        226  CTCGAG               C^TCGAG
              884  CTCGAG              
PflFI        1321  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1498  GACCAAGTC           
SexAI        1059  ACCAGGT              A^CC[AT]GGT
             1601  ACCAGGT             
SspBI        2654  TGTACA               T^GTACA
             2674  TGTACA              
SspI          152  AATATT               AAT^ATT
             1299  AATATT              
TfiI          436  GATTC                G^A[AT]TC
             2185  GAATC               
Tth111I      1321  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1498  GACCAAGTC           
XhoI          226  CTCGAG               C^TCGAG
              884  CTCGAG              
XhoII         507  AGATCT               [GA]^GATC[CT]
              960  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AvaI        AvaII       BanI        
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MseI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XmnI