################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AclI AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbvCI BciVI BclI BglI BlnI BsaAI BsaBI BsaI BsiEI BsiWI BspDI BspLU11I BsrDI BsrFI BssHII BssSI BstAPI BstBI Bsu36I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MscI MunI NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccIII 1994 TCCGGA T^CCGGA AflII 1610 CTTAAG C^TTAAG AlwNI 1189 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1987 AGCGCT AGC^GCT AspHI 2432 GTGCTC G[AT]GC[AT]^C BbeI 142 GGCGCC GGCGC^C BfaI 2472 CTAG C^TAG BfrI 1610 CTTAAG C^TTAAG BsaWI 1994 TCCGGA [AT]^CCGG[AT] BseAI 1994 TCCGGA T^CCGGA BsiHKAI 2432 GTGCTC G[AT]GC[AT]^C BspEI 1994 TCCGGA T^CCGGA BspHI 1177 TCATGA T^CATGA BspMI 2097 ACCTGC ACCTGC(4/8) BsrBI 206 GAGCGG CCGCTC(-3/-3) Bst1107I 545 GTATAC GTA^TAC BstEII 2463 GGTGACC G^GT[ACGT]ACC BstPI 2463 GGTGACC G^GT[ACGT]ACC BstZ17I 545 GTATAC GTA^TAC DrdI 1568 GACACTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 975 TGGCCG [CT]^GGCC[GA] Eco47III 1987 AGCGCT AGC^GCT EcoNI 282 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2463 GGTGACC G^GT[ACGT]ACC KasI 142 GGCGCC G^GCGCC MaeI 2472 CTAG C^TAG MaeII 2363 ACGT A^CGT MroI 1994 TCCGGA T^CCGGA MspA1I 215 CAGCGG C[AC]G^C[GT]G NarI 142 GGCGCC GG^CGCC NcoI 2328 CCATGG C^CATGG NspI 1968 GCATGT [GA]CATG^[CT] RcaI 1177 TCATGA T^CATGA SfoI 142 GGCGCC GGC^GCC StuI 1361 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 545 GTATAC GT^[AC][GT]AC 1037 GTAGAC AccII 75 CGCG CG^CG 521 CGCG AcyI 6 GACGCC G[GA]^CG[CT]C 142 GGCGCC AspI 1321 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1498 GACCAAGTC BanII 1202 GAGCCC G[GA]GC[CT]^C 2084 GGGCTC BcgI 123 CGATGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2091 CGAAGGACCTGC BglII 507 AGATCT A^GATCT 960 AGATCT BlpI 1886 GCTGAGC GC^T[ACGT]AGC 2077 GCTTAGC Bpu1102I 1886 GCTGAGC GC^T[ACGT]AGC 2077 GCTTAGC BsaHI 6 GACGCC G[GA]^CG[CT]C 142 GGCGCC BseRI 97 CTCCTC GAGGAG(10/8) 2104 GAGGAG BsgI 781 GTGCAG GTGCAG(16/14) 945 GTGCAG BsmBI 4 GAGACG CGTCTC(1/5) 71 GAGACG BsmI 397 GAATGC GAATGC(1/-1) 2696 GAATGC Bsp1407I 2654 TGTACA T^GTACA 2674 TGTACA BsrGI 2654 TGTACA T^GTACA 2674 TGTACA BstUI 75 CGCG CG^CG 521 CGCG BstXI 1935 CCAAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2139 CCATACCTCTGG BstYI 507 AGATCT [GA]^GATC[CT] 960 AGATCT CelII 1886 GCTGAGC GC^T[ACGT]AGC 2077 GCTTAGC DraI 470 TTTAAA TTT^AAA 922 TTTAAA DsaI 870 CCACGG C^C[GA][CT]GG 2328 CCATGG FauI 482 GCGGG CCCGC(4/6) 2082 GCGGG Hin1I 6 GACGCC G[GA]^CG[CT]C 142 GGCGCC HincII 1085 GTTGAC GT[CT]^[GA]AC 1565 GTTGAC HindII 1085 GTTGAC GT[CT]^[GA]AC 1565 GTTGAC MflI 507 AGATCT [GA]^GATC[CT] 960 AGATCT MslI 536 CATGCCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1324 CATGTCGATG MvnI 75 CGCG CG^CG 521 CGCG NdeI 1863 CATATG CA^TATG 2670 CATATG PaeR7I 226 CTCGAG C^TCGAG 884 CTCGAG PflFI 1321 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1498 GACCAAGTC SexAI 1059 ACCAGGT A^CC[AT]GGT 1601 ACCAGGT SspBI 2654 TGTACA T^GTACA 2674 TGTACA SspI 152 AATATT AAT^ATT 1299 AATATT TfiI 436 GATTC G^A[AT]TC 2185 GAATC Tth111I 1321 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1498 GACCAAGTC XhoI 226 CTCGAG C^TCGAG 884 CTCGAG XhoII 507 AGATCT [GA]^GATC[CT] 960 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AvaI AvaII BanI BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XmnI