##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BfrI BlnI BsaAI BsaBI
BsaI BseAI BsiWI BsmI BspEI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I CpoI DraI Eam1105I EarI
EcoNI EcoRV FseI FspI HindIII
HpaI KpnI Ksp632I MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI ScaI SfiI SfuI
SmlI SnaBI SphI SspI SwaI
Tth111I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 344 GACGTC GACGT^C
AcsI 524 GAATTC [GA]^AATT[CT]
AflIII 1836 ACACGT A^C[GA][CT]GT
Aor51HI 1716 AGCGCT AGC^GCT
ApoI 524 GAATTC [GA]^AATT[CT]
AspHI 1031 GAGCTC G[AT]GC[AT]^C
BbvCI 2168 CCTCAGC CCTCAGC(-5/-2)
BclI 1608 TGATCA T^GATCA
BglII 1020 AGATCT A^GATCT
BsiEI 378 CGGCCG CG[GA][CT]^CG
BsiHKAI 1031 GAGCTC G[AT]GC[AT]^C
BsmAI 1736 GAGAC GTCTC(1/5)
BsmBI 1736 GAGACG CGTCTC(1/5)
BspDI 1373 ATCGAT AT^CGAT
BstAPI 1054 GCAGGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 1373 ATCGAT AT^CGAT
EagI 378 CGGCCG C^GGCCG
EclXI 378 CGGCCG C^GGCCG
Eco47III 1716 AGCGCT AGC^GCT
Eco52I 378 CGGCCG C^GGCCG
EcoRI 524 GAATTC G^AATTC
EcoT22I 737 ATGCAT ATGCA^T
FbaI 1608 TGATCA T^GATCA
HincII 937 GTTGAC GT[CT]^[GA]AC
HindII 937 GTTGAC GT[CT]^[GA]AC
NsiI 737 ATGCAT ATGCA^T
NspI 735 ACATGC [GA]CATG^[CT]
SacI 1031 GAGCTC GAGCT^C
SbfI 1183 CCTGCAGG CCTGCA^GG
SexAI 1011 ACCTGGT A^CC[AT]GGT
SgrAI 1766 CACCGGCG C[GA]^CCGG[CT]G
SpeI 2501 ACTAGT A^CTAGT
Sse8387I 1183 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AocI 182 CCTGAGG CC^T[ACGT]AGG
2354 CCTCAGG
BalI 753 TGGCCA TGG^CCA
1378 TGGCCA
BbeI 15 GGCGCC GGCGC^C
1072 GGCGCC
BbsI 428 GTCTTC GAAGAC(2/6)
845 GTCTTC
BcgI 945 CGAACTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
657 GCAATGCCATCG
BciVI 2327 GGATAC GTATCC(6/5)
492 GTATCC
BfaI 132 CTAG C^TAG
2502 CTAG
BglI 530 GCCTACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1203 GCCTGGCCGGC
BpuAI 428 GTCTTC GAAGAC(2/6)
845 GTCTTC
BseRI 764 GAGGAG GAGGAG(10/8)
1028 GAGGAG
Bsp1407I 2042 TGTACA T^GTACA
2401 TGTACA
BspHI 309 TCATGA T^CATGA
576 TCATGA
BsrBI 1198 GAGCGG CCGCTC(-3/-3)
1448 GAGCGG
BsrDI 657 GCAATG GCAATG(2/0)
1616 GCAATG
BsrGI 2042 TGTACA T^GTACA
2401 TGTACA
BstEII 796 GGTGACC G^GT[ACGT]ACC
1891 GGTGACC
BstPI 796 GGTGACC G^GT[ACGT]ACC
1891 GGTGACC
Bsu36I 182 CCTGAGG CC^T[ACGT]AGG
2354 CCTCAGG
DrdI 605 GACAGCGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1007 GACTACCTGGTC
Eco81I 182 CCTGAGG CC^T[ACGT]AGG
2354 CCTCAGG
EcoO65I 796 GGTGACC G^GT[ACGT]ACC
1891 GGTGACC
KasI 15 GGCGCC G^GCGCC
1072 GGCGCC
KspI 1101 CCGCGG CCGC^GG
1193 CCGCGG
MaeI 132 CTAG C^TAG
2502 CTAG
MluNI 753 TGGCCA TGG^CCA
1378 TGGCCA
MscI 753 TGGCCA TGG^CCA
1378 TGGCCA
MseI 1563 TTAA T^TAA
2123 TTAA
NaeI 1208 GCCGGC GCC^GGC
2296 GCCGGC
NarI 15 GGCGCC GG^CGCC
1072 GGCGCC
NgoMIV 1208 GCCGGC G^CCGGC
2296 GCCGGC
PpuMI 64 GGGTCCC [GA]G^G[AT]CC[CT]
2279 GGGTCCC
RcaI 309 TCATGA T^CATGA
576 TCATGA
SacII 1101 CCGCGG CCGC^GG
1193 CCGCGG
SfoI 15 GGCGCC GGC^GCC
1072 GGCGCC
SmaI 720 CCCGGG CCC^GGG
2180 CCCGGG
SspBI 2042 TGTACA T^GTACA
2401 TGTACA
Tru9I 1563 TTAA T^TAA
2123 TTAA
XmaCI 720 CCCGGG C^CCGGG
2180 CCCGGG
XmaI 720 CCCGGG C^CCGGG
2180 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BanI
BanII BbvI BcnI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BsaWI
BsgI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII