##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AlwNI       ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvrII       BalI        BamHI       BbeI        
BbrPI       BfrI        BglII       BlnI        BsaAI       
BsaWI       BseAI       BsiWI       BspDI       BspEI       
BssHII      Bst1107I    BstZ17I     ClaI        CpoI        
DraIII      Eam1105I    EcoT22I     FseI        HindIII     
HpaI        KasI        KpnI        KspI        MluI        
MluNI       MroI        MscI        NarI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII        1258  GACGTC               GACGT^C
Aor51HI      2210  AGCGCT               AGC^GCT
Asp700I      2305  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         815  TGCGCA               TGC^GCA
BcgI         2492  CGAGGCTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI          69  GGATAC               GTATCC(6/5)
BclI         2150  TGATCA               T^GATCA
BglI         2583  GCCCACTAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI          53  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI           1  CGGCCG               CG[GA][CT]^CG
BsmI         1965  GAATGC               GAATGC(1/-1)
Bsp1407I     1667  TGTACA               T^GTACA
BspHI        2516  TCATGA               T^CATGA
BsrDI        2073  CATTGC               GCAATG(2/0)
BsrGI        1667  TGTACA               T^GTACA
BssSI        1609  CACGAG               CACGAG(-5/-1)
BstBI         944  TTCGAA               TT^CGAA
BstEII       1618  GGTGACC              G^GT[ACGT]ACC
BstPI        1618  GGTGACC              G^GT[ACGT]ACC
DrdI          578  GACCCCCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI            1  CGGCCG               [CT]^GGCC[GA]
EagI            1  CGGCCG               C^GGCCG
EclXI           1  CGGCCG               C^GGCCG
Eco47III     2210  AGCGCT               AGC^GCT
Eco52I          1  CGGCCG               C^GGCCG
EcoNI        2229  CCTGAATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1618  GGTGACC              G^GT[ACGT]ACC
EcoRI         494  GAATTC               G^AATTC
EcoRV        2200  GATATC               GAT^ATC
FbaI         2150  TGATCA               T^GATCA
FspI          815  TGCGCA               TGC^GCA
MamI           53  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          937  CAATTG               C^AATTG
MunI          937  CAATTG               C^AATTG
NaeI           16  GCCGGC               GCC^GGC
NcoI          740  CCATGG               C^CATGG
NgoMIV         16  GCCGGC               G^CCGGC
NspV          944  TTCGAA               TT^CGAA
PflMI         291  CCAGCGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          667  CTGCAG               CTGCA^G
RcaI         2516  TCATGA               T^CATGA
SacI           88  GAGCTC               GAGCT^C
ScaI         1712  AGTACT               AGT^ACT
SfuI          944  TTCGAA               TT^CGAA
SpeI         1870  ACTAGT               A^CTAGT
SphI         1040  GCATGC               GCATG^C
SspBI        1667  TGTACA               T^GTACA
StuI          836  AGGCCT               AGG^CCT
Van91I        291  CCAGCGATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2486  CCAATGCGAGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2305  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AocI         1200  CCTCAGG              CC^T[ACGT]AGG
             1725  CCTCAGG             
ApaI         1196  GGGCCC               GGGCC^C
             2581  GGGCCC              
AspHI          88  GAGCTC               G[AT]GC[AT]^C
             1592  GTGCTC              
AvaI          256  CTCGGG               C^[CT]CG[GA]G
              263  CTCGGG              
BbsI          523  GAAGAC               GAAGAC(2/6)
             1402  GAAGAC              
BbvCI        1602  CCTCAGC              CCTCAGC(-5/-2)
              439  GCTGAGG             
BcnI          416  CCCGG                CC^[GC]GG
             1673  CCCGG               
BlpI         1594  GCTCAGC              GC^T[ACGT]AGC
             2001  GCTGAGC             
Bpu1102I     1594  GCTCAGC              GC^T[ACGT]AGC
             2001  GCTGAGC             
BpuAI         523  GAAGAC               GAAGAC(2/6)
             1402  GAAGAC              
BsaI         1052  GAGACC               GGTCTC(1/5)
              260  GGTCTC              
BsiHKAI        88  GAGCTC               G[AT]GC[AT]^C
             1592  GTGCTC              
BsmBI          34  GAGACG               CGTCTC(1/5)
              226  GAGACG              
BsoBI         256  CTCGGG               C^[CT]CG[GA]G
              263  CTCGGG              
BspLU11I      731  ACATGT               A^CATGT
             1757  ACATGT              
BspMI         665  ACCTGC               ACCTGC(4/8)
             1055  ACCTGC              
BsrBI          26  GAGCGG               CCGCTC(-3/-3)
             1513  GAGCGG              
BsrFI          16  GCCGGC               [GA]^CCGG[CT]
              774  ACCGGC              
BstXI        1608  CCACGAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2585  CCACTAGGCTGG        
Bsu36I       1200  CCTCAGG              CC^T[ACGT]AGG
             1725  CCTCAGG             
CelII        1594  GCTCAGC              GC^T[ACGT]AGC
             2001  GCTGAGC             
Cfr10I         16  GCCGGC               [GA]^CCGG[CT]
              774  ACCGGC              
DraI         1108  TTTAAA               TTT^AAA
             1572  TTTAAA              
DsaI          740  CCATGG               C^C[GA][CT]GG
             1688  CCACGG              
Eco81I       1200  CCTCAGG              CC^T[ACGT]AGG
             1725  CCTCAGG             
HincII        768  GTTGAC               GT[CT]^[GA]AC
             2653  GTCAAC              
HindII        768  GTTGAC               GT[CT]^[GA]AC
             2653  GTCAAC              
MslI          606  CATCGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              873  CACCCTTGTG          
MspA1I        910  CAGCTG               C[AC]G^C[GT]G
             1096  CAGCTG              
NciI          416  CCCGG                CC^[GC]GG
             1673  CCCGG               
PvuII         910  CAGCTG               CAG^CTG
             1096  CAGCTG              
SexAI         677  ACCTGGT              A^CC[AT]GGT
             1114  ACCTGGT             
TfiI         1812  GATTC                G^A[AT]TC
             2232  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        ApoI        AvaII       
BanI        BanII       BbvI        BfaI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII