##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AclI AcsI AflII AgeI AhdI
ApoI AscI AseI AsnI Asp700I
AspEI AvrII BbvCI BciVI BclI
BfrI BlnI BsaBI BsiWI BsmBI
BsmI BspDI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eam1105I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SapI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspI SwaI Tsp509I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1829 GGTACC G^GTACC
AccII 1826 CGCG CG^CG
AccIII 203 TCCGGA T^CCGGA
AflIII 1944 ACACGT A^C[GA][CT]GT
ApaI 659 GGGCCC GGGCC^C
Asp718I 1829 GGTACC G^GTACC
AviII 1394 TGCGCA TGC^GCA
BamHI 608 GGATCC G^GATCC
BbeI 689 GGCGCC GGCGC^C
BbsI 2005 GTCTTC GAAGAC(2/6)
BfaI 2046 CTAG C^TAG
BglII 1959 AGATCT A^GATCT
BpmI 1354 CTGGAG CTGGAG(16/14)
BpuAI 2005 GTCTTC GAAGAC(2/6)
BseAI 203 TCCGGA T^CCGGA
Bsp1407I 1889 TGTACA T^GTACA
BspEI 203 TCCGGA T^CCGGA
BspHI 500 TCATGA T^CATGA
BsrGI 1889 TGTACA T^GTACA
BstAPI 122 GCACTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1826 CGCG CG^CG
BstXI 593 CCATCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 508 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 1394 TGCGCA TGC^GCA
KasI 689 GGCGCC G^GCGCC
KpnI 1829 GGTACC GGTAC^C
KspI 1825 CCGCGG CCGC^GG
MaeI 2046 CTAG C^TAG
MroI 203 TCCGGA T^CCGGA
MseI 89 TTAA T^TAA
MvnI 1826 CGCG CG^CG
NarI 689 GGCGCC GG^CGCC
NcoI 1878 CCATGG C^CATGG
RcaI 500 TCATGA T^CATGA
SacI 1639 GAGCTC GAGCT^C
SacII 1825 CCGCGG CCGC^GG
SalI 478 GTCGAC G^TCGAC
SbfI 1696 CCTGCAGG CCTGCA^GG
SfoI 689 GGCGCC GGC^GCC
SmaI 302 CCCGGG CCC^GGG
Sse8387I 1696 CCTGCAGG CCTGCA^GG
SspBI 1889 TGTACA T^GTACA
StuI 1358 AGGCCT AGG^CCT
Tru9I 89 TTAA T^TAA
XmaCI 302 CCCGGG C^CCGGG
XmaI 302 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 496 GACGTC GACGT^C
1975 GACGTC
Alw44I 586 GTGCAC G^TGCAC
1690 GTGCAC
AocI 961 CCTGAGG CC^T[ACGT]AGG
1321 CCTGAGG
Aor51HI 785 AGCGCT AGC^GCT
902 AGCGCT
ApaLI 586 GTGCAC G^TGCAC
1690 GTGCAC
AspI 1048 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1705 GACAACGTC
BcgI 992 CGAGGGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1862 GCAGTGTCATCG
BglI 615 GCCTCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1376 GCCAAGTGGGC
BsaI 629 GAGACC GGTCTC(1/5)
573 GGTCTC
BsaWI 203 TCCGGA [AT]^CCGG[AT]
1055 TCCGGT
BsiEI 527 CGGCCG CG[GA][CT]^CG
988 CGGCCG
BstEII 513 GGTGACC G^GT[ACGT]ACC
1425 GGTGACC
BstPI 513 GGTGACC G^GT[ACGT]ACC
1425 GGTGACC
Bsu36I 961 CCTGAGG CC^T[ACGT]AGG
1321 CCTGAGG
DrdI 1858 GACGGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2023 GACGGGACTGTC
EagI 527 CGGCCG C^GGCCG
988 CGGCCG
EarI 2038 CTCTTC CTCTTC(1/4)
1179 GAAGAG
EclXI 527 CGGCCG C^GGCCG
988 CGGCCG
Eco47III 785 AGCGCT AGC^GCT
902 AGCGCT
Eco52I 527 CGGCCG C^GGCCG
988 CGGCCG
Eco81I 961 CCTGAGG CC^T[ACGT]AGG
1321 CCTGAGG
EcoO65I 513 GGTGACC G^GT[ACGT]ACC
1425 GGTGACC
HincII 478 GTCGAC GT[CT]^[GA]AC
2088 GTCAAC
HindII 478 GTCGAC GT[CT]^[GA]AC
2088 GTCAAC
Ksp632I 2038 CTCTTC CTCTTC(1/4)
1179 GAAGAG
PflFI 1048 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1705 GACAACGTC
PvuII 1297 CAGCTG CAG^CTG
1501 CAGCTG
TaqI 479 TCGA T^CGA
899 TCGA
TfiI 1 GATTC G^A[AT]TC
1494 GATTC
Tth111I 1048 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1705 GACAACGTC
TthHB8I 479 TCGA T^CGA
899 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbvI
BcnI BlpI BmyI Bpu1102I BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI Tsp45I TspRI
Van91I XhoII