##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AclI        AcsI        AflII       AgeI        AhdI        
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AvrII       BbvCI       BciVI       BclI        
BfrI        BlnI        BsaBI       BsiWI       BsmBI       
BsmI        BspDI       BspLU11I    BsrBI       BsrDI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        DraI        Eam1105I    EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HindIII     HpaI        MamI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SapI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        SspI        SwaI        Tsp509I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I       1829  GGTACC               G^GTACC
AccII        1826  CGCG                 CG^CG
AccIII        203  TCCGGA               T^CCGGA
AflIII       1944  ACACGT               A^C[GA][CT]GT
ApaI          659  GGGCCC               GGGCC^C
Asp718I      1829  GGTACC               G^GTACC
AviII        1394  TGCGCA               TGC^GCA
BamHI         608  GGATCC               G^GATCC
BbeI          689  GGCGCC               GGCGC^C
BbsI         2005  GTCTTC               GAAGAC(2/6)
BfaI         2046  CTAG                 C^TAG
BglII        1959  AGATCT               A^GATCT
BpmI         1354  CTGGAG               CTGGAG(16/14)
BpuAI        2005  GTCTTC               GAAGAC(2/6)
BseAI         203  TCCGGA               T^CCGGA
Bsp1407I     1889  TGTACA               T^GTACA
BspEI         203  TCCGGA               T^CCGGA
BspHI         500  TCATGA               T^CATGA
BsrGI        1889  TGTACA               T^GTACA
BstAPI        122  GCACTCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1826  CGCG                 CG^CG
BstXI         593  CCATCAAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII        508  CACCCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
FspI         1394  TGCGCA               TGC^GCA
KasI          689  GGCGCC               G^GCGCC
KpnI         1829  GGTACC               GGTAC^C
KspI         1825  CCGCGG               CCGC^GG
MaeI         2046  CTAG                 C^TAG
MroI          203  TCCGGA               T^CCGGA
MseI           89  TTAA                 T^TAA
MvnI         1826  CGCG                 CG^CG
NarI          689  GGCGCC               GG^CGCC
NcoI         1878  CCATGG               C^CATGG
RcaI          500  TCATGA               T^CATGA
SacI         1639  GAGCTC               GAGCT^C
SacII        1825  CCGCGG               CCGC^GG
SalI          478  GTCGAC               G^TCGAC
SbfI         1696  CCTGCAGG             CCTGCA^GG
SfoI          689  GGCGCC               GGC^GCC
SmaI          302  CCCGGG               CCC^GGG
Sse8387I     1696  CCTGCAGG             CCTGCA^GG
SspBI        1889  TGTACA               T^GTACA
StuI         1358  AGGCCT               AGG^CCT
Tru9I          89  TTAA                 T^TAA
XmaCI         302  CCCGGG               C^CCGGG
XmaI          302  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AatII         496  GACGTC               GACGT^C
             1975  GACGTC              
Alw44I        586  GTGCAC               G^TGCAC
             1690  GTGCAC              
AocI          961  CCTGAGG              CC^T[ACGT]AGG
             1321  CCTGAGG             
Aor51HI       785  AGCGCT               AGC^GCT
              902  AGCGCT              
ApaLI         586  GTGCAC               G^TGCAC
             1690  GTGCAC              
AspI         1048  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1705  GACAACGTC           
BcgI          992  CGAGGGACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1862  GCAGTGTCATCG        
BglI          615  GCCTCGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1376  GCCAAGTGGGC         
BsaI          629  GAGACC               GGTCTC(1/5)
              573  GGTCTC              
BsaWI         203  TCCGGA               [AT]^CCGG[AT]
             1055  TCCGGT              
BsiEI         527  CGGCCG               CG[GA][CT]^CG
              988  CGGCCG              
BstEII        513  GGTGACC              G^GT[ACGT]ACC
             1425  GGTGACC             
BstPI         513  GGTGACC              G^GT[ACGT]ACC
             1425  GGTGACC             
Bsu36I        961  CCTGAGG              CC^T[ACGT]AGG
             1321  CCTGAGG             
DrdI         1858  GACGGCAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2023  GACGGGACTGTC        
EagI          527  CGGCCG               C^GGCCG
              988  CGGCCG              
EarI         2038  CTCTTC               CTCTTC(1/4)
             1179  GAAGAG              
EclXI         527  CGGCCG               C^GGCCG
              988  CGGCCG              
Eco47III      785  AGCGCT               AGC^GCT
              902  AGCGCT              
Eco52I        527  CGGCCG               C^GGCCG
              988  CGGCCG              
Eco81I        961  CCTGAGG              CC^T[ACGT]AGG
             1321  CCTGAGG             
EcoO65I       513  GGTGACC              G^GT[ACGT]ACC
             1425  GGTGACC             
HincII        478  GTCGAC               GT[CT]^[GA]AC
             2088  GTCAAC              
HindII        478  GTCGAC               GT[CT]^[GA]AC
             2088  GTCAAC              
Ksp632I      2038  CTCTTC               CTCTTC(1/4)
             1179  GAAGAG              
PflFI        1048  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1705  GACAACGTC           
PvuII        1297  CAGCTG               CAG^CTG
             1501  CAGCTG              
TaqI          479  TCGA                 T^CGA
              899  TCGA                
TfiI            1  GATTC                G^A[AT]TC
             1494  GATTC               
Tth111I      1048  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1705  GACAACGTC           
TthHB8I       479  TCGA                 T^CGA
              899  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccI        AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbrPI       BbvI        
BcnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MwoI        NaeI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       PflMI       
PleI        PmaCI       PmlI        PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TseI        Tsp45I      TspRI       
Van91I      XhoII