################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp700I AspEI AvrII BbvCI BciVI BclI BfrI BlnI BsaBI BsiWI BsmBI BsmI BspDI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoNI EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SapI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI SspI SwaI Tsp509I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 1829 GGTACC G^GTACC AccII 1826 CGCG CG^CG AccIII 203 TCCGGA T^CCGGA AflIII 1944 ACACGT A^C[GA][CT]GT ApaI 659 GGGCCC GGGCC^C Asp718I 1829 GGTACC G^GTACC AviII 1394 TGCGCA TGC^GCA BamHI 608 GGATCC G^GATCC BbeI 689 GGCGCC GGCGC^C BbsI 2005 GTCTTC GAAGAC(2/6) BfaI 2046 CTAG C^TAG BglII 1959 AGATCT A^GATCT BpmI 1354 CTGGAG CTGGAG(16/14) BpuAI 2005 GTCTTC GAAGAC(2/6) BseAI 203 TCCGGA T^CCGGA Bsp1407I 1889 TGTACA T^GTACA BspEI 203 TCCGGA T^CCGGA BspHI 500 TCATGA T^CATGA BsrGI 1889 TGTACA T^GTACA BstAPI 122 GCACTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1826 CGCG CG^CG BstXI 593 CCATCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraIII 508 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG FspI 1394 TGCGCA TGC^GCA KasI 689 GGCGCC G^GCGCC KpnI 1829 GGTACC GGTAC^C KspI 1825 CCGCGG CCGC^GG MaeI 2046 CTAG C^TAG MroI 203 TCCGGA T^CCGGA MseI 89 TTAA T^TAA MvnI 1826 CGCG CG^CG NarI 689 GGCGCC GG^CGCC NcoI 1878 CCATGG C^CATGG RcaI 500 TCATGA T^CATGA SacI 1639 GAGCTC GAGCT^C SacII 1825 CCGCGG CCGC^GG SalI 478 GTCGAC G^TCGAC SbfI 1696 CCTGCAGG CCTGCA^GG SfoI 689 GGCGCC GGC^GCC SmaI 302 CCCGGG CCC^GGG Sse8387I 1696 CCTGCAGG CCTGCA^GG SspBI 1889 TGTACA T^GTACA StuI 1358 AGGCCT AGG^CCT Tru9I 89 TTAA T^TAA XmaCI 302 CCCGGG C^CCGGG XmaI 302 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AatII 496 GACGTC GACGT^C 1975 GACGTC Alw44I 586 GTGCAC G^TGCAC 1690 GTGCAC AocI 961 CCTGAGG CC^T[ACGT]AGG 1321 CCTGAGG Aor51HI 785 AGCGCT AGC^GCT 902 AGCGCT ApaLI 586 GTGCAC G^TGCAC 1690 GTGCAC AspI 1048 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1705 GACAACGTC BcgI 992 CGAGGGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1862 GCAGTGTCATCG BglI 615 GCCTCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1376 GCCAAGTGGGC BsaI 629 GAGACC GGTCTC(1/5) 573 GGTCTC BsaWI 203 TCCGGA [AT]^CCGG[AT] 1055 TCCGGT BsiEI 527 CGGCCG CG[GA][CT]^CG 988 CGGCCG BstEII 513 GGTGACC G^GT[ACGT]ACC 1425 GGTGACC BstPI 513 GGTGACC G^GT[ACGT]ACC 1425 GGTGACC Bsu36I 961 CCTGAGG CC^T[ACGT]AGG 1321 CCTGAGG DrdI 1858 GACGGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2023 GACGGGACTGTC EagI 527 CGGCCG C^GGCCG 988 CGGCCG EarI 2038 CTCTTC CTCTTC(1/4) 1179 GAAGAG EclXI 527 CGGCCG C^GGCCG 988 CGGCCG Eco47III 785 AGCGCT AGC^GCT 902 AGCGCT Eco52I 527 CGGCCG C^GGCCG 988 CGGCCG Eco81I 961 CCTGAGG CC^T[ACGT]AGG 1321 CCTGAGG EcoO65I 513 GGTGACC G^GT[ACGT]ACC 1425 GGTGACC HincII 478 GTCGAC GT[CT]^[GA]AC 2088 GTCAAC HindII 478 GTCGAC GT[CT]^[GA]AC 2088 GTCAAC Ksp632I 2038 CTCTTC CTCTTC(1/4) 1179 GAAGAG PflFI 1048 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1705 GACAACGTC PvuII 1297 CAGCTG CAG^CTG 1501 CAGCTG TaqI 479 TCGA T^CGA 899 TCGA TfiI 1 GATTC G^A[AT]TC 1494 GATTC Tth111I 1048 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1705 GACAACGTC TthHB8I 479 TCGA T^CGA 899 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccI AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvI BcnI BlpI BmyI Bpu1102I BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PmaCI PmlI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI Van91I XhoII