##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BlnI BpuAI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BsrFI
BssHII BssSI Bst1107I BstZ17I Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SgrAI
SmaI SnaBI SpeI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1796 GTCTAC GT^[AC][GT]AC
AccII 1763 CGCG CG^CG
Alw44I 592 GTGCAC G^TGCAC
ApaLI 592 GTGCAC G^TGCAC
BbeI 2526 GGCGCC GGCGC^C
BbvCI 2009 CCTCAGC CCTCAGC(-5/-2)
BglII 1400 AGATCT A^GATCT
BsgI 475 CTGCAC GTGCAG(16/14)
BsmI 1528 GCATTC GAATGC(1/-1)
Bsp1407I 2046 TGTACA T^GTACA
BsrBI 1063 GAGCGG CCGCTC(-3/-3)
BsrDI 758 GCAATG GCAATG(2/0)
BsrGI 2046 TGTACA T^GTACA
BstAPI 2342 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1814 TTCGAA TT^CGAA
BstEII 1500 GGTCACC G^GT[ACGT]ACC
BstPI 1500 GGTCACC G^GT[ACGT]ACC
BstUI 1763 CGCG CG^CG
DraIII 1993 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 277 GACTCAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1500 GGTCACC G^GT[ACGT]ACC
KasI 2526 GGCGCC G^GCGCC
MaeII 2513 ACGT A^CGT
MseI 2318 TTAA T^TAA
MvnI 1763 CGCG CG^CG
NarI 2526 GGCGCC GG^CGCC
NspI 310 GCATGC [GA]CATG^[CT]
NspV 1814 TTCGAA TT^CGAA
SbfI 1191 CCTGCAGG CCTGCA^GG
SfoI 2526 GGCGCC GGC^GCC
SfuI 1814 TTCGAA TT^CGAA
SmlI 739 CTCAAG C^T[CT][GA]AG
SphI 310 GCATGC GCATG^C
Sse8387I 1191 CCTGCAGG CCTGCA^GG
SspBI 2046 TGTACA T^GTACA
TfiI 2644 GAATC G^A[AT]TC
Tru9I 2318 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 1793 GGCGTC G[GA]^CG[CT]C
2526 GGCGCC
Asp700I 1398 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2099 GAAGGCTTTC
BcnI 98 CCCGG CC^[GC]GG
2266 CCCGG
BlpI 259 GCTGAGC GC^T[ACGT]AGC
1203 GCTGAGC
Bpu1102I 259 GCTGAGC GC^T[ACGT]AGC
1203 GCTGAGC
BsaHI 1793 GGCGTC G[GA]^CG[CT]C
2526 GGCGCC
BsaWI 20 ACCGGA [AT]^CCGG[AT]
971 TCCGGT
BstXI 425 CCAATGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1727 CCATGGCCATGG
CelII 259 GCTGAGC GC^T[ACGT]AGC
1203 GCTGAGC
EcoNI 81 CCTAGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2371 CCTCTGTGAGG
EcoRI 93 GAATTC G^AATTC
399 GAATTC
Hin1I 1793 GGCGTC G[GA]^CG[CT]C
2526 GGCGCC
NciI 98 CCCGG CC^[GC]GG
2266 CCCGG
PpuMI 866 AGGTCCC [GA]G^G[AT]CC[CT]
2344 AGGACCT
SapI 1462 GAAGAGC GCTCTTC(1/4)
537 GCTCTTC
XcmI 1115 CCAAGATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1661 CCATGCCAGCCATGG
XmnI 1398 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2099 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaII BalI BanI BanII BbvI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII