##################################
# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I AlwI AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsgI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII Cfr13I
ClaI CpoI DdeI DpnII DraII
DraIII DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MunI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI Sau96I SbfI
ScaI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI TaqI
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 174 GGCGTC G[GA]^CG[CT]C
AflIII 589 ACATGT A^C[GA][CT]GT
AgeI 163 ACCGGT A^CCGGT
Aor51HI 376 AGCGCT AGC^GCT
Asp700I 403 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 160 GGCACC G^G[CT][GA]CC
BanII 196 GAGCCC G[GA]GC[CT]^C
BfaI 382 CTAG C^TAG
BmyI 196 GAGCCC G[AGT]GC[ACT]^C
BpmI 193 CTGGAG CTGGAG(16/14)
BsaAI 217 TACGTG [CT]AC^GT[GA]
BsaHI 174 GGCGTC G[GA]^CG[CT]C
BsaJI 11 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 163 ACCGGT [AT]^CCGG[AT]
BseRI 262 GAGGAG GAGGAG(10/8)
BsiEI 165 CGGTCG CG[GA][CT]^CG
BsmFI 177 GTCCC GGGAC(10/14)
Bsp1286I 196 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 589 ACATGT A^CATGT
BspMI 309 ACCTGC ACCTGC(4/8)
BsrFI 163 ACCGGT [GA]^CCGG[CT]
BsrI 122 CCAGT ACTGG(1/-1)
Cac8I 171 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 377 GCGC GCG^C
Cfr10I 163 ACCGGT [GA]^CCGG[CT]
DrdI 493 GACACAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 376 AGCGCT AGC^GCT
EcoT14I 11 CCTTGG C^C[AT][AT]GG
FauI 83 GCGGG CCCGC(4/6)
Fnu4HI 137 GCGGC GC^[ACGT]GC
HaeII 376 AGCGCT [GA]GCGC^[CT]
HaeIII 9 GGCC GG^CC
HgaI 175 GCGTC GACGC(5/10)
HhaI 377 GCGC GCG^C
Hin1I 174 GGCGTC G[GA]^CG[CT]C
HinP1I 377 GCGC G^CGC
ItaI 137 GCGGC GC^[ACGT]GC
MaeI 382 CTAG C^TAG
MaeII 218 ACGT A^CGT
MaeIII 586 GTTAC ^GT[ACGT]AC
MwoI 128 GCTTATTTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII 590 CATG CATG^
NspI 589 ACATGT [GA]CATG^[CT]
PinAI 163 ACCGGT A^CCGGT
SexAI 447 ACCTGGT A^CC[AT]GGT
SfcI 102 CTACAG C^T[GA][CT]AG
SspI 693 AATATT AAT^ATT
StyI 11 CCTTGG C^C[AT][AT]GG
SwaI 711 ATTTAAAT ATTT^AAAT
XcmI 147 CCATTCCAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 403 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 82 CGCG CG^CG
136 CGCG
AluI 127 AGCT AG^CT
605 AGCT
BbsI 97 GAAGAC GAAGAC(2/6)
490 GAAGAC
BpuAI 97 GAAGAC GAAGAC(2/6)
490 GAAGAC
BsiYI 11 CCTTGGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
67 CCTGACGACGG
BslI 11 CCTTGGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
67 CCTGACGACGG
BssKI 51 CCAGG ^CC[ACGT]GG
448 CCTGG
BstF5I 278 CATCC GGATG(2/0)
20 GGATG
BstNI 51 CCAGG CC^[AT]GG
448 CCTGG
BstUI 82 CGCG CG^CG
136 CGCG
EcoRII 51 CCAGG ^CC[AT]GG
448 CCTGG
FokI 278 CATCC GGATG(9/13)
20 GGATG
HapII 94 CCGG C^CGG
164 CCGG
HpaII 94 CCGG C^CGG
164 CCGG
HphI 747 GGTGA GGTGA(8/7)
411 TCACC
MspI 94 CCGG C^CGG
164 CCGG
MvaI 51 CCAGG CC^[AT]GG
448 CCTGG
MvnI 82 CGCG CG^CG
136 CGCG
NlaIV 160 GGCACC GG[ACGT]^[ACGT]CC
195 GGAGCC
PleI 824 GACTC GAGTC(4/5)
87 GAGTC
ScrFI 51 CCAGG CC^[ACGT]GG
448 CCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI AcsI AfaI ApoI DraI
EarI HinfI Ksp632I MboII MnlI
MseI RsaI TfiI Tru9I Tsp509I