##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BclI BcnI
BglI BlnI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FbaI FseI FspI HgaI Hin1I
HindIII KasI KpnI KspI MamI
MfeI MluNI MroI MscI MunI
NarI NciI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SmaI
SnaBI SpeI SphI SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccI 1285 GTCTAC GT^[AC][GT]AC
AccII 1228 CGCG CG^CG
AflII 2409 CTTAAG C^TTAAG
AlwI 2293 GATCC GGATC(4/5)
Aor51HI 62 AGCGCT AGC^GCT
ApaI 1398 GGGCCC GGGCC^C
Asp700I 1078 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2211 CTCGAG C^[CT]CG[GA]G
BanI 756 GGCACC G^G[CT][GA]CC
BciVI 1907 GGATAC GTATCC(6/5)
BfrI 2409 CTTAAG C^TTAAG
BglII 1564 AGATCT A^GATCT
BlpI 150 GCTGAGC GC^T[ACGT]AGC
BpmI 3093 CTGGAG CTGGAG(16/14)
Bpu1102I 150 GCTGAGC GC^T[ACGT]AGC
BsaAI 2770 CACGTA [CT]AC^GT[GA]
BsaI 3213 GGTCTC GGTCTC(1/5)
BsmBI 1139 CGTCTC CGTCTC(1/5)
BsoBI 2211 CTCGAG C^[CT]CG[GA]G
Bsp1407I 374 TGTACA T^GTACA
BspLU11I 557 ACATGT A^CATGT
BsrDI 3128 CATTGC GCAATG(2/0)
BsrFI 12 GCCGGC [GA]^CCGG[CT]
BsrGI 374 TGTACA T^GTACA
BstAPI 901 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1228 CGCG CG^CG
BstXI 2928 CCAGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1564 AGATCT [GA]^GATC[CT]
CelII 150 GCTGAGC GC^T[ACGT]AGC
CfoI 63 GCGC GCG^C
Cfr10I 12 GCCGGC [GA]^CCGG[CT]
DrdI 106 GACGAGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 74 CCATGG C^C[GA][CT]GG
Eco47III 62 AGCGCT AGC^GCT
EcoNI 2543 CCTAAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 74 CCATGG C^C[AT][AT]GG
EcoT22I 734 ATGCAT ATGCA^T
HaeII 62 AGCGCT [GA]GCGC^[CT]
HapII 13 CCGG C^CGG
HhaI 63 GCGC GCG^C
HinP1I 63 GCGC G^CGC
HpaII 13 CCGG C^CGG
MflI 1564 AGATCT [GA]^GATC[CT]
MluI 1227 ACGCGT A^CGCGT
MspI 13 CCGG C^CGG
MvnI 1228 CGCG CG^CG
NaeI 12 GCCGGC GCC^GGC
NcoI 74 CCATGG C^CATGG
NdeI 1826 CATATG CA^TATG
NgoMIV 12 GCCGGC G^CCGGC
NheI 348 GCTAGC G^CTAGC
NsiI 734 ATGCAT ATGCA^T
PaeR7I 2211 CTCGAG C^TCGAG
PshAI 1439 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 907 CCTGCAGG CCTGCA^GG
SgrAI 11 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 907 CCTGCAGG CCTGCA^GG
SspBI 374 TGTACA T^GTACA
SspI 2422 AATATT AAT^ATT
StuI 912 AGGCCT AGG^CCT
StyI 74 CCATGG C^C[AT][AT]GG
XbaI 1561 TCTAGA T^CTAGA
XhoI 2211 CTCGAG C^TCGAG
XhoII 1564 AGATCT [GA]^GATC[CT]
XmnI 1078 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AocI 1962 CCTGAGG CC^T[ACGT]AGG
2154 CCTCAGG
BsgI 1302 CTGCAC GTGCAG(16/14)
485 GTGCAG
BspMI 612 ACCTGC ACCTGC(4/8)
624 GCAGGT
Bsu36I 1962 CCTGAGG CC^T[ACGT]AGG
2154 CCTCAGG
Eco81I 1962 CCTGAGG CC^T[ACGT]AGG
2154 CCTCAGG
EcoRI 1761 GAATTC G^AATTC
1870 GAATTC
HpaI 175 GTTAAC GTT^AAC
3392 GTTAAC
MspA1I 488 CAGCTG C[AC]G^C[GT]G
2841 CAGCTG
NspI 246 GCATGT [GA]CATG^[CT]
557 ACATGT
PflMI 1776 CCATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2076 CCAGGACGTGG
PpuMI 55 GGGTCCC [GA]G^G[AT]CC[CT]
2119 GGGACCT
PvuII 488 CAGCTG CAG^CTG
2841 CAGCTG
TaqI 2212 TCGA T^CGA
3217 TCGA
TthHB8I 2212 TCGA T^CGA
3217 TCGA
Van91I 1776 CCATTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2076 CCAGGACGTGG
XcmI 641 CCAAAGGAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1735 CCAAAATTATACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BfaI BmyI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI