##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AclI AflIII Alw44I
AocI Aor51HI ApaLI AscI Asp700I
AspI AvaI AviII AvrII BalI
BbvCI BcgI BclI BcnI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraIII DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
FbaI FseI FspI HgaI KspI
MamI MluI MluNI MroI MscI
NaeI NciI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PmeI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 627 GTCTAC GT^[AC][GT]AC
AcyI 230 GGCGCC G[GA]^CG[CT]C
AflII 3350 CTTAAG C^TTAAG
AgeI 757 ACCGGT A^CCGGT
AhdI 1056 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1171 GGGCCC GGGCC^C
AspEI 1056 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2181 GGATCC G^GATCC
BanII 1171 GGGCCC G[GA]GC[CT]^C
BbeI 230 GGCGCC GGCGC^C
BbrPI 1 CACGTG CAC^GTG
BciVI 2593 GTATCC GTATCC(6/5)
BfrI 3350 CTTAAG C^TTAAG
BglI 233 GCCAGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 230 GGCGCC G[GA]^CG[CT]C
BsaI 615 GAGACC GGTCTC(1/5)
BsaWI 757 ACCGGT [AT]^CCGG[AT]
BsmBI 153 GAGACG CGTCTC(1/5)
Bsp1407I 3412 TGTACA T^GTACA
BsrBI 1279 CCGCTC CCGCTC(-3/-3)
BsrGI 3412 TGTACA T^GTACA
EaeI 157 CGGCCA [CT]^GGCC[GA]
Eam1105I 1056 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 993 CCTGGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1220 GAATTC G^AATTC
EcoT22I 1630 ATGCAT ATGCA^T
HaeII 230 GGCGCC [GA]GCGC^[CT]
Hin1I 230 GGCGCC G[GA]^CG[CT]C
HindIII 1740 AAGCTT A^AGCTT
HpaI 1619 GTTAAC GTT^AAC
KasI 230 GGCGCC G^GCGCC
MfeI 2909 CAATTG C^AATTG
MunI 2909 CAATTG C^AATTG
NarI 230 GGCGCC GG^CGCC
NdeI 1137 CATATG CA^TATG
NsiI 1630 ATGCAT ATGCA^T
PinAI 757 ACCGGT A^CCGGT
PmaCI 1 CACGTG CAC^GTG
PmlI 1 CACGTG CAC^GTG
PpuMI 76 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2627 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 873 GAAGAGC GCTCTTC(1/4)
ScaI 1469 AGTACT AGT^ACT
SexAI 1657 ACCTGGT A^CC[AT]GGT
SfoI 230 GGCGCC GGC^GCC
SpeI 2482 ACTAGT A^CTAGT
SspBI 3412 TGTACA T^GTACA
StuI 106 AGGCCT AGG^CCT
SwaI 2883 ATTTAAAT ATTT^AAAT
TaqI 590 TCGA T^CGA
TthHB8I 590 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 207 GGTACC G^GTACC
1661 GGTACC
AccII 194 CGCG CG^CG
238 CGCG
AseI 386 ATTAAT AT^TAAT
2941 ATTAAT
AsnI 386 ATTAAT AT^TAAT
2941 ATTAAT
Asp718I 207 GGTACC G^GTACC
1661 GGTACC
BsaAI 1 CACGTG [CT]AC^GT[GA]
183 TACGTG
BstUI 194 CGCG CG^CG
238 CGCG
BstYI 2033 AGATCC [GA]^GATC[CT]
2181 GGATCC
DraII 76 GGGTCCC [GA]G^G[ACGT]CC[CT]
1171 GGGCCCC
DrdI 405 GACAACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2299 GACATCAGTGTC
EcoO109I 76 GGGTCCC [GA]G^G[ACGT]CC[CT]
1171 GGGCCCC
EcoT14I 250 CCAAGG C^C[AT][AT]GG
2145 CCAAGG
KpnI 207 GGTACC GGTAC^C
1661 GGTACC
MflI 2033 AGATCC [GA]^GATC[CT]
2181 GGATCC
MvnI 194 CGCG CG^CG
238 CGCG
PshBI 386 ATTAAT AT^TAAT
2941 ATTAAT
PstI 8 CTGCAG CTGCA^G
243 CTGCAG
StyI 250 CCAAGG C^C[AT][AT]GG
2145 CCAAGG
Tsp45I 136 GTCAC ^GT[GC]AC
665 GTCAC
XcmI 19 CCAGAGCAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
704 CCAGCCCTTTGATGG
XhoII 2033 AGATCC [GA]^GATC[CT]
2181 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TfiI Tru9I TseI
Tsp509I TspRI Van91I