##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       AclI        AflII       AflIII      AgeI        
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        AspEI       AspI        AvrII       BbeI        
BfrI        BglII       BlnI        BsaBI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspLU11I    BsrGI       
BssHII      Bst1107I    BstZ17I     CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      FseI        
HindIII     KasI        KspI        MamI        MfeI        
MluI        MunI        NarI        NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SfoI        
SnaBI       Sse8387I    SspBI       SspI        SwaI        
Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       4990  GGTACC               G^GTACC
AccII        5083  CGCG                 CG^CG
AcyI         5470  GACGCC               G[GA]^CG[CT]C
Asp718I      4990  GGTACC               G^GTACC
BamHI         763  GGATCC               G^GATCC
BbrPI        3240  CACGTG               CAC^GTG
BciVI        3167  GGATAC               GTATCC(6/5)
BglI         2622  GCCACGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        3240  CACGTG               [CT]AC^GT[GA]
BsaHI        5470  GACGCC               G[GA]^CG[CT]C
BspDI        2902  ATCGAT               AT^CGAT
BsrBI        4269  GAGCGG               CCGCTC(-3/-3)
BssSI        2229  CACGAG               CACGAG(-5/-1)
BstBI         518  TTCGAA               TT^CGAA
BstEII       3902  GGTAACC              G^GT[ACGT]ACC
BstPI        3902  GGTAACC              G^GT[ACGT]ACC
BstUI        5083  CGCG                 CG^CG
ClaI         2902  ATCGAT               AT^CGAT
DraI          704  TTTAAA               TTT^AAA
DrdI         4922  GACGTTAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3902  GGTAACC              G^GT[ACGT]ACC
EcoRI        2835  GAATTC               G^AATTC
Hin1I        5470  GACGCC               G[GA]^CG[CT]C
HpaI         1784  GTTAAC               GTT^AAC
KpnI         4990  GGTACC               GGTAC^C
MvnI         5083  CGCG                 CG^CG
NcoI         4948  CCATGG               C^CATGG
NdeI         5223  CATATG               CA^TATG
NspV          518  TTCGAA               TT^CGAA
PmaCI        3240  CACGTG               CAC^GTG
PmlI         3240  CACGTG               CAC^GTG
SacI         5043  GAGCTC               GAGCT^C
SapI          521  GAAGAGC              GCTCTTC(1/4)
SfiI         2621  GGCCACGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI          518  TTCGAA               TT^CGAA
SgrAI           1  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI         5017  CCCGGG               CCC^GGG
SpeI          493  ACTAGT               A^CTAGT
XmaCI        5017  CCCGGG               C^CCGGG
XmaI         5017  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI         3873  GTAGAC               GT^[AC][GT]AC
             4173  GTCTAC              
AccIII       1012  TCCGGA               T^CCGGA
             1235  TCCGGA              
Alw44I       3464  GTGCAC               G^TGCAC
             5010  GTGCAC              
ApaLI        3464  GTGCAC               G^TGCAC
             5010  GTGCAC              
Asp700I      2787  GAACTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3043  GAACCCTTTC          
AvaI          300  CTCGGG               C^[CT]CG[GA]G
             5017  CCCGGG              
AviII         877  TGCGCA               TGC^GCA
             2722  TGCGCA              
BbsI         3587  GAAGAC               GAAGAC(2/6)
             5441  GTCTTC              
BcgI         2904  CGATACTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              816  GCATGGATATCG        
BclI         2044  TGATCA               T^GATCA
             2416  TGATCA              
BpuAI        3587  GAAGAC               GAAGAC(2/6)
             5441  GTCTTC              
BsaI         2766  GAGACC               GGTCTC(1/5)
             3693  GAGACC              
BsaWI        1012  TCCGGA               [AT]^CCGG[AT]
             1235  TCCGGA              
BseAI        1012  TCCGGA               T^CCGGA
             1235  TCCGGA              
BsoBI         300  CTCGGG               C^[CT]CG[GA]G
             5017  CCCGGG              
BspEI        1012  TCCGGA               T^CCGGA
             1235  TCCGGA              
BsrFI           2  GCCGGC               [GA]^CCGG[CT]
             5520  GCCGGC              
Cfr10I          2  GCCGGC               [GA]^CCGG[CT]
             5520  GCCGGC              
EcoRV         821  GATATC               GAT^ATC
             4413  GATATC              
EcoT22I       654  ATGCAT               ATGCA^T
             1118  ATGCAT              
FbaI         2044  TGATCA               T^GATCA
             2416  TGATCA              
FspI          877  TGCGCA               TGC^GCA
             2722  TGCGCA              
HaeII         579  AGCGCC               [GA]GCGC^[CT]
             2149  GGCGCT              
MroI         1012  TCCGGA               T^CCGGA
             1235  TCCGGA              
NaeI            2  GCCGGC               GCC^GGC
             5520  GCCGGC              
NgoMIV          2  GCCGGC               G^CCGGC
             5520  GCCGGC              
NsiI          654  ATGCAT               ATGCA^T
             1118  ATGCAT              
SexAI         744  ACCTGGT              A^CC[AT]GGT
             1570  ACCTGGT             
SmlI         1528  CTCAAG               C^T[CT][GA]AG
             4650  CTCAAG              
SphI         1116  GCATGC               GCATG^C
             2951  GCATGC              
XbaI         2741  TCTAGA               T^CTAGA
             3944  TCTAGA              
XmnI         2787  GAACTGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3043  GAACCCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  119 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       AocI        ApoI        AspHI       AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BcnI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BspHI       BspMI       BsrDI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RcaI        RsaI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII