##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AvrII BbeI
BfrI BglII BlnI BsaBI BsiEI
BsiWI BsmBI Bsp1407I BspLU11I BsrGI
BssHII Bst1107I BstZ17I CpoI EagI
Eam1105I EclXI Eco47III Eco52I FseI
HindIII KasI KspI MamI MfeI
MluI MunI NarI NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfoI
SnaBI Sse8387I SspBI SspI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 4990 GGTACC G^GTACC
AccII 5083 CGCG CG^CG
AcyI 5470 GACGCC G[GA]^CG[CT]C
Asp718I 4990 GGTACC G^GTACC
BamHI 763 GGATCC G^GATCC
BbrPI 3240 CACGTG CAC^GTG
BciVI 3167 GGATAC GTATCC(6/5)
BglI 2622 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3240 CACGTG [CT]AC^GT[GA]
BsaHI 5470 GACGCC G[GA]^CG[CT]C
BspDI 2902 ATCGAT AT^CGAT
BsrBI 4269 GAGCGG CCGCTC(-3/-3)
BssSI 2229 CACGAG CACGAG(-5/-1)
BstBI 518 TTCGAA TT^CGAA
BstEII 3902 GGTAACC G^GT[ACGT]ACC
BstPI 3902 GGTAACC G^GT[ACGT]ACC
BstUI 5083 CGCG CG^CG
ClaI 2902 ATCGAT AT^CGAT
DraI 704 TTTAAA TTT^AAA
DrdI 4922 GACGTTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3902 GGTAACC G^GT[ACGT]ACC
EcoRI 2835 GAATTC G^AATTC
Hin1I 5470 GACGCC G[GA]^CG[CT]C
HpaI 1784 GTTAAC GTT^AAC
KpnI 4990 GGTACC GGTAC^C
MvnI 5083 CGCG CG^CG
NcoI 4948 CCATGG C^CATGG
NdeI 5223 CATATG CA^TATG
NspV 518 TTCGAA TT^CGAA
PmaCI 3240 CACGTG CAC^GTG
PmlI 3240 CACGTG CAC^GTG
SacI 5043 GAGCTC GAGCT^C
SapI 521 GAAGAGC GCTCTTC(1/4)
SfiI 2621 GGCCACGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 518 TTCGAA TT^CGAA
SgrAI 1 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 5017 CCCGGG CCC^GGG
SpeI 493 ACTAGT A^CTAGT
XmaCI 5017 CCCGGG C^CCGGG
XmaI 5017 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3873 GTAGAC GT^[AC][GT]AC
4173 GTCTAC
AccIII 1012 TCCGGA T^CCGGA
1235 TCCGGA
Alw44I 3464 GTGCAC G^TGCAC
5010 GTGCAC
ApaLI 3464 GTGCAC G^TGCAC
5010 GTGCAC
Asp700I 2787 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3043 GAACCCTTTC
AvaI 300 CTCGGG C^[CT]CG[GA]G
5017 CCCGGG
AviII 877 TGCGCA TGC^GCA
2722 TGCGCA
BbsI 3587 GAAGAC GAAGAC(2/6)
5441 GTCTTC
BcgI 2904 CGATACTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
816 GCATGGATATCG
BclI 2044 TGATCA T^GATCA
2416 TGATCA
BpuAI 3587 GAAGAC GAAGAC(2/6)
5441 GTCTTC
BsaI 2766 GAGACC GGTCTC(1/5)
3693 GAGACC
BsaWI 1012 TCCGGA [AT]^CCGG[AT]
1235 TCCGGA
BseAI 1012 TCCGGA T^CCGGA
1235 TCCGGA
BsoBI 300 CTCGGG C^[CT]CG[GA]G
5017 CCCGGG
BspEI 1012 TCCGGA T^CCGGA
1235 TCCGGA
BsrFI 2 GCCGGC [GA]^CCGG[CT]
5520 GCCGGC
Cfr10I 2 GCCGGC [GA]^CCGG[CT]
5520 GCCGGC
EcoRV 821 GATATC GAT^ATC
4413 GATATC
EcoT22I 654 ATGCAT ATGCA^T
1118 ATGCAT
FbaI 2044 TGATCA T^GATCA
2416 TGATCA
FspI 877 TGCGCA TGC^GCA
2722 TGCGCA
HaeII 579 AGCGCC [GA]GCGC^[CT]
2149 GGCGCT
MroI 1012 TCCGGA T^CCGGA
1235 TCCGGA
NaeI 2 GCCGGC GCC^GGC
5520 GCCGGC
NgoMIV 2 GCCGGC G^CCGGC
5520 GCCGGC
NsiI 654 ATGCAT ATGCA^T
1118 ATGCAT
SexAI 744 ACCTGGT A^CC[AT]GGT
1570 ACCTGGT
SmlI 1528 CTCAAG C^T[CT][GA]AG
4650 CTCAAG
SphI 1116 GCATGC GCATG^C
2951 GCATGC
XbaI 2741 TCTAGA T^CTAGA
3944 TCTAGA
XmnI 2787 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3043 GAACCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII