##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
Acc65I AccIII AclI AscI Asp718I
AspI BglII BsaBI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BssHII ClaI CpoI EagI EclXI
Eco52I EcoRI HpaI KpnI MamI
MluI MroI NotI NruI PacI
PflFI PshAI Psp1406I PvuI RcaI
RsrII SalI SexAI SnaBI SpeI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3643 GACGTC GACGT^C
AhdI 1349 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 7025 ATTAAT AT^TAAT
AsnI 7025 ATTAAT AT^TAAT
Asp700I 2410 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1349 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 5154 TGCGCA TGC^GCA
BalI 1075 TGGCCA TGG^CCA
BamHI 6668 GGATCC G^GATCC
BcgI 708 CGATGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 6247 GGATAC GTATCC(6/5)
BclI 3794 TGATCA T^GATCA
BsaI 3367 GGTCTC GGTCTC(1/5)
Bsp1407I 4142 TGTACA T^GTACA
BspLU11I 5378 ACATGT A^CATGT
BsrGI 4142 TGTACA T^GTACA
Bst1107I 4157 GTATAC GTA^TAC
BstBI 2399 TTCGAA TT^CGAA
BstEII 190 GGTGACC G^GT[ACGT]ACC
BstPI 190 GGTGACC G^GT[ACGT]ACC
BstZ17I 4157 GTATAC GTA^TAC
Eam1105I 1349 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 190 GGTGACC G^GT[ACGT]ACC
EcoRV 3462 GATATC GAT^ATC
EcoT22I 3591 ATGCAT ATGCA^T
FbaI 3794 TGATCA T^GATCA
FseI 1409 GGCCGGCC GGCCGG^CC
FspI 5154 TGCGCA TGC^GCA
KspI 2658 CCGCGG CCGC^GG
MluNI 1075 TGGCCA TGG^CCA
MscI 1075 TGGCCA TGG^CCA
NdeI 3816 CATATG CA^TATG
NsiI 3591 ATGCAT ATGCA^T
NspV 2399 TTCGAA TT^CGAA
PmeI 4821 GTTTAAAC GTTT^AAAC
PshBI 7025 ATTAAT AT^TAAT
SacII 2658 CCGCGG CCGC^GG
SbfI 1153 CCTGCAGG CCTGCA^GG
ScaI 5827 AGTACT AGT^ACT
SfiI 1018 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2399 TTCGAA TT^CGAA
SgrAI 6951 CACCGGTG C[GA]^CCGG[CT]G
SphI 2781 GCATGC GCATG^C
Sse8387I 1153 CCTGCAGG CCTGCA^GG
SspBI 4142 TGTACA T^GTACA
XcmI 2836 CCACAGCACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2410 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AgeI 3832 ACCGGT A^CCGGT
6952 ACCGGT
Alw44I 434 GTGCAC G^TGCAC
4707 GTGCAC
Aor51HI 769 AGCGCT AGC^GCT
2447 AGCGCT
ApaLI 434 GTGCAC G^TGCAC
4707 GTGCAC
AvrII 3443 CCTAGG C^CTAGG
4029 CCTAGG
BbeI 1159 GGCGCC GGCGC^C
4518 GGCGCC
BglI 1019 GCCGCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1951 GCCCTACCGGC
BlnI 3443 CCTAGG C^CTAGG
4029 CCTAGG
BsmBI 2566 CGTCTC CGTCTC(1/5)
1607 GAGACG
BsrDI 4653 CATTGC GCAATG(2/0)
3431 GCAATG
DrdI 1467 GACCAAACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2993 GACACGTCCGTC
Eco47III 769 AGCGCT AGC^GCT
2447 AGCGCT
EcoNI 1897 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5455 CCTACTTGAGG
HgaI 1609 GACGC GACGC(5/10)
2332 GACGC
KasI 1159 GGCGCC G^GCGCC
4518 GGCGCC
MfeI 3653 CAATTG C^AATTG
6050 CAATTG
MunI 3653 CAATTG C^AATTG
6050 CAATTG
NarI 1159 GGCGCC GG^CGCC
4518 GGCGCC
NcoI 132 CCATGG C^CATGG
1234 CCATGG
NheI 2716 GCTAGC G^CTAGC
5719 GCTAGC
PaeR7I 1853 CTCGAG C^TCGAG
5329 CTCGAG
PinAI 3832 ACCGGT A^CCGGT
6952 ACCGGT
PpuMI 47 GGGTCCT [GA]G^G[AT]CC[CT]
650 AGGTCCC
SapI 2851 GAAGAGC GCTCTTC(1/4)
3233 GAAGAGC
SfoI 1159 GGCGCC GGC^GCC
4518 GGCGCC
XhoI 1853 CTCGAG C^TCGAG
5329 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvCI BbvI BcnI BfaI
BfrI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI