##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AhdI Aor51HI ApaI AscI AspEI
AviII AvrII BbeI BcgI BcnI
BglI BlnI BlpI Bpu1102I BsaHI
BsaI BseAI BsiWI BsmBI BspDI
BspEI BsrBI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CelII
CfoI ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I FseI FspI HaeII
HhaI Hin1I HinP1I KasI KspI
MluI MroI MvnI NarI NciI
NotI NruI PacI PmeI PpuMI
PshAI Psp1406I RsrII SacII SalI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AgeI 836 ACCGGT A^CCGGT
Alw44I 1851 GTGCAC G^TGCAC
AocI 6464 CCTCAGG CC^T[ACGT]AGG
ApaLI 1851 GTGCAC G^TGCAC
AspI 1304 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 5823 CTCGAG C^[CT]CG[GA]G
BamHI 4729 GGATCC G^GATCC
BbrPI 1090 CACGTG CAC^GTG
BbvCI 3284 GCTGAGG CCTCAGC(-5/-2)
BciVI 6174 GTATCC GTATCC(6/5)
BsiEI 5396 CGATCG CG[GA][CT]^CG
BsoBI 5823 CTCGAG C^[CT]CG[GA]G
BssSI 6029 CACGAG CACGAG(-5/-1)
BstXI 4638 CCAAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 6464 CCTCAGG CC^T[ACGT]AGG
DraIII 4016 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 6464 CCTCAGG CC^T[ACGT]AGG
FauI 6723 GCGGG CCCGC(4/6)
HgaI 1532 GACGC GACGC(5/10)
NaeI 4586 GCCGGC GCC^GGC
NgoMIV 4586 GCCGGC G^CCGGC
PaeR7I 5823 CTCGAG C^TCGAG
PflFI 1304 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2090 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 836 ACCGGT A^CCGGT
PmaCI 1090 CACGTG CAC^GTG
PmlI 1090 CACGTG CAC^GTG
PvuI 5396 CGATCG CGAT^CG
SexAI 5650 ACCTGGT A^CC[AT]GGT
Tth111I 1304 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2090 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4873 TCTAGA T^CTAGA
XhoI 5823 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Asp700I 1046 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4851 GAAGAATTTC
BalI 2087 TGGCCA TGG^CCA
4647 TGGCCA
BclI 2247 TGATCA T^GATCA
6268 TGATCA
BsaBI 551 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2151 GATTGGCATC
BsaWI 200 ACCGGA [AT]^CCGG[AT]
836 ACCGGT
Bsp1407I 2436 TGTACA T^GTACA
6874 TGTACA
BspHI 3690 TCATGA T^CATGA
4032 TCATGA
BspMI 6087 ACCTGC ACCTGC(4/8)
6190 GCAGGT
BsrDI 5019 CATTGC GCAATG(2/0)
3447 GCAATG
BsrGI 2436 TGTACA T^GTACA
6874 TGTACA
BstBI 5032 TTCGAA TT^CGAA
5090 TTCGAA
EcoRI 5517 GAATTC G^AATTC
5524 GAATTC
FbaI 2247 TGATCA T^GATCA
6268 TGATCA
HpaI 5122 GTTAAC GTT^AAC
5803 GTTAAC
MamI 551 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2151 GATTGGCATC
MluNI 2087 TGGCCA TGG^CCA
4647 TGGCCA
MscI 2087 TGGCCA TGG^CCA
4647 TGGCCA
MslI 2314 CACCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3443 CACTGCAATG
NspV 5032 TTCGAA TT^CGAA
5090 TTCGAA
RcaI 3690 TCATGA T^CATGA
4032 TCATGA
SacI 1713 GAGCTC GAGCT^C
5826 GAGCTC
ScaI 1321 AGTACT AGT^ACT
4367 AGTACT
SfuI 5032 TTCGAA TT^CGAA
5090 TTCGAA
SphI 317 GCATGC GCATG^C
6619 GCATGC
SspBI 2436 TGTACA T^GTACA
6874 TGTACA
StuI 291 AGGCCT AGG^CCT
3009 AGGCCT
XcmI 3348 CCAAACAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3722 CCATATGAAGAGTGG
XmnI 1046 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4851 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
Acc65I AccI AciI AcsI AfaI
AflII AflIII AluI AlwI AlwNI
ApoI AseI AsnI Asp718I AspHI
AvaII BanI BanII BbsI BbvI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoNI EcoRII
EcoRV EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HincII HindII HindIII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MspA1I MspI MunI MvaI MwoI
NcoI NdeI NdeII NheI NlaIII
NlaIV NsiI NspI PleI PshBI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII