##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccII AccIII AclI AflIII
AgeI AhdI Alw44I AocI ApaLI
AscI Asp700I AspEI AspI AviII
BalI BbeI BbrPI BcgI BciVI
BglI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BstBI BstEII BstPI BstUI
Bsu36I Cfr10I ClaI CpoI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI KasI KspI MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2032 GGTACC G^GTACC
AcyI 1669 GACGCC G[GA]^CG[CT]C
AflII 93 CTTAAG C^TTAAG
Aor51HI 89 AGCGCT AGC^GCT
ApaI 1697 GGGCCC GGGCC^C
AseI 919 ATTAAT AT^TAAT
AsnI 919 ATTAAT AT^TAAT
Asp718I 2032 GGTACC G^GTACC
AvaI 1664 CTCGGG C^[CT]CG[GA]G
AvrII 1951 CCTAGG C^CTAGG
BbvCI 1088 CCTCAGC CCTCAGC(-5/-2)
BclI 426 TGATCA T^GATCA
BfrI 93 CTTAAG C^TTAAG
BglII 642 AGATCT A^GATCT
BlnI 1951 CCTAGG C^CTAGG
BlpI 85 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 85 GCTGAGC GC^T[ACGT]AGC
BsaHI 1669 GACGCC G[GA]^CG[CT]C
BsaI 1158 GGTCTC GGTCTC(1/5)
BsgI 944 CTGCAC GTGCAG(16/14)
BsmI 1638 GAATGC GAATGC(1/-1)
BsoBI 1664 CTCGGG C^[CT]CG[GA]G
Bst1107I 2250 GTATAC GTA^TAC
BstAPI 57 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2250 GTATAC GTA^TAC
CelII 85 GCTGAGC GC^T[ACGT]AGC
CfoI 90 GCGC GCG^C
Eco47III 89 AGCGCT AGC^GCT
FbaI 426 TGATCA T^GATCA
HaeII 89 AGCGCT [GA]GCGC^[CT]
HgaI 1669 GACGC GACGC(5/10)
HhaI 90 GCGC GCG^C
Hin1I 1669 GACGCC G[GA]^CG[CT]C
HinP1I 90 GCGC G^CGC
HincII 1252 GTTAAC GT[CT]^[GA]AC
HindII 1252 GTTAAC GT[CT]^[GA]AC
HindIII 988 AAGCTT A^AGCTT
HpaI 1252 GTTAAC GTT^AAC
HphI 1595 GGTGA GGTGA(8/7)
KpnI 2032 GGTACC GGTAC^C
MfeI 1346 CAATTG C^AATTG
MunI 1346 CAATTG C^AATTG
NspI 62 GCATGC [GA]CATG^[CT]
PflMI 721 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 389 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 919 ATTAAT AT^TAAT
SacI 215 GAGCTC GAGCT^C
SapI 1135 GAAGAGC GCTCTTC(1/4)
SphI 62 GCATGC GCATG^C
SspI 862 AATATT AAT^ATT
Van91I 721 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 363 GTCTAC GT^[AC][GT]AC
2250 GTATAC
AciI 1395 CCGC CCGC(-3/-1)
1865 GCGG
BamHI 221 GGATCC G^GATCC
1751 GGATCC
BfaI 337 CTAG C^TAG
1952 CTAG
Bsp1407I 1579 TGTACA T^GTACA
2257 TGTACA
BspMI 293 ACCTGC ACCTGC(4/8)
1002 ACCTGC
BsrGI 1579 TGTACA T^GTACA
2257 TGTACA
BssSI 256 CTCGTG CACGAG(-5/-1)
1542 CTCGTG
BstXI 536 CCAAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2201 CCACAGGGGTGG
DraI 79 TTTAAA TTT^AAA
2071 TTTAAA
DraII 684 AGGCCCT [GA]G^G[ACGT]CC[CT]
1697 GGGCCCT
DraIII 1521 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1614 CACCTTGTG
EcoNI 255 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
688 CCTGGAAGAGG
EcoO109I 684 AGGCCCT [GA]G^G[ACGT]CC[CT]
1697 GGGCCCT
MaeI 337 CTAG C^TAG
1952 CTAG
NcoI 266 CCATGG C^CATGG
1438 CCATGG
PstI 1004 CTGCAG CTGCA^G
1730 CTGCAG
ScaI 1719 AGTACT AGT^ACT
1982 AGTACT
SmlI 93 CTTAAG C^T[CT][GA]AG
1986 CTCAAG
SspBI 1579 TGTACA T^GTACA
2257 TGTACA
StuI 851 AGGCCT AGG^CCT
1784 AGGCCT
TaqI 387 TCGA T^CGA
1636 TCGA
Tsp45I 290 GTGAC ^GT[GC]AC
1259 GTCAC
TthHB8I 387 TCGA T^CGA
1636 TCGA
XcmI 266 CCATGGAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
536 CCAAAATCCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DrdI DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII