##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BglI BlnI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Cfr10I ClaI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoT22I FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeII
MamI MluI MluNI MscI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 757 TCCGGA T^CCGGA
AfaI 33 GTAC GT^AC
AflII 2890 CTTAAG C^TTAAG
AflIII 109 ACATGT A^C[GA][CT]GT
AocI 2340 CCTGAGG CC^T[ACGT]AGG
Asp700I 264 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 2443 TGATCA T^GATCA
BfrI 2890 CTTAAG C^TTAAG
BglII 831 AGATCT A^GATCT
BlpI 1153 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1153 GCTCAGC GC^T[ACGT]AGC
BsaWI 757 TCCGGA [AT]^CCGG[AT]
BseAI 757 TCCGGA T^CCGGA
BspEI 757 TCCGGA T^CCGGA
BspHI 3133 TCATGA T^CATGA
BspLU11I 109 ACATGT A^CATGT
BstXI 1780 CCATGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 831 AGATCT [GA]^GATC[CT]
Bsu36I 2340 CCTGAGG CC^T[ACGT]AGG
CelII 1153 GCTCAGC GC^T[ACGT]AGC
CpoI 79 CGGACCG CG^G[AT]CCG
DraI 2709 TTTAAA TTT^AAA
Eco81I 2340 CCTGAGG CC^T[ACGT]AGG
EcoRI 846 GAATTC G^AATTC
EcoRV 3216 GATATC GAT^ATC
FbaI 2443 TGATCA T^GATCA
HincII 1106 GTCAAC GT[CT]^[GA]AC
HindII 1106 GTCAAC GT[CT]^[GA]AC
MflI 831 AGATCT [GA]^GATC[CT]
MroI 757 TCCGGA T^CCGGA
NdeI 2496 CATATG CA^TATG
RcaI 3133 TCATGA T^CATGA
RsaI 33 GTAC GT^AC
RsrII 79 CGGACCG CG^G[AT]CCG
SapI 540 GAAGAGC GCTCTTC(1/4)
SexAI 1571 ACCTGGT A^CC[AT]GGT
XbaI 3140 TCTAGA T^CTAGA
XhoII 831 AGATCT [GA]^GATC[CT]
XmnI 264 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 1156 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1249 CAGGCCCTG
AseI 3076 ATTAAT AT^TAAT
3160 ATTAAT
AsnI 3076 ATTAAT AT^TAAT
3160 ATTAAT
AspHI 1436 GAGCAC G[AT]GC[AT]^C
2161 GAGCAC
BanI 1095 GGCACC G^G[CT][GA]CC
1776 GGCACC
BanII 636 GAGCCC G[GA]GC[CT]^C
1382 GGGCTC
BpmI 781 CTCCAG CTGGAG(16/14)
1223 CTGGAG
BsgI 2021 GTGCAG GTGCAG(16/14)
2662 GTGCAG
BsiHKAI 1436 GAGCAC G[AT]GC[AT]^C
2161 GAGCAC
BsmI 1007 GAATGC GAATGC(1/-1)
3151 GAATGC
BstEII 7 GGTGACC G^GT[ACGT]ACC
1071 GGTGACC
BstPI 7 GGTGACC G^GT[ACGT]ACC
1071 GGTGACC
EcoO65I 7 GGTGACC G^GT[ACGT]ACC
1071 GGTGACC
FauI 87 CCCGC CCCGC(4/6)
92 GCGGG
MfeI 733 CAATTG C^AATTG
2610 CAATTG
MunI 733 CAATTG C^AATTG
2610 CAATTG
NcoI 1780 CCATGG C^CATGG
2623 CCATGG
PpuMI 212 GGGACCT [GA]G^G[AT]CC[CT]
1739 GGGACCC
PshBI 3076 ATTAAT AT^TAAT
3160 ATTAAT
PvuII 1120 CAGCTG CAG^CTG
1156 CAGCTG
SfcI 2800 CTGTAG C^T[GA][CT]AG
3035 CTGTAG
SphI 1756 GCATGC GCATG^C
1897 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaII BbsI BbvI BcnI
BfaI BmyI BpuAI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI Sau3AI Sau96I ScrFI SfaNI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I