##################################
# NO CUT ENZYMES (   51 enzymes) #
##################################

Acc65I      AflII       AgeI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp718I     AviII       
BbrPI       BclI        BfrI        BsaAI       BsaBI       
BsiWI       BsmBI       BssHII      Bst1107I    BstBI       
BstZ17I     EcoRI       EcoRV       FbaI        FseI        
FspI        HpaI        KpnI        MamI        MluI        
NdeI        NheI        NotI        NruI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshBI       PvuI        SbfI        ScaI        SfuI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AclI         1885  AACGTT               AA^CGTT
AhdI         2170  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI        59  AGCGCT               AGC^GCT
Asp700I      2367  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2170  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        4487  CCTAGG               C^CTAGG
BglII         252  AGATCT               A^GATCT
BlnI         4487  CCTAGG               C^CTAGG
BlpI          906  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      906  GCTGAGC              GC^T[ACGT]AGC
BsiEI           4  CGGCCG               CG[GA][CT]^CG
BspDI        3391  ATCGAT               AT^CGAT
BspLU11I     2252  ACATGT               A^CATGT
BstXI        2543  CCACGGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         906  GCTGAGC              GC^T[ACGT]AGC
ClaI         3391  ATCGAT               AT^CGAT
CpoI         2878  CGGTCCG              CG^G[AT]CCG
EagI            4  CGGCCG               C^GGCCG
Eam1105I     2170  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI           4  CGGCCG               C^GGCCG
Eco47III       59  AGCGCT               AGC^GCT
Eco52I          4  CGGCCG               C^GGCCG
MfeI          422  CAATTG               C^AATTG
MunI          422  CAATTG               C^AATTG
PaeR7I        529  CTCGAG               C^TCGAG
Psp1406I     1885  AACGTT               AA^CGTT
RsrII        2878  CGGTCCG              CG^G[AT]CCG
SalI         2137  GTCGAC               G^TCGAC
SfiI          126  GGCCGTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI        2227  CACCGGCG             C[GA]^CCGG[CT]G
XbaI          456  TCTAGA               T^CTAGA
XhoI          529  CTCGAG               C^TCGAG
XmnI         2367  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AatII        1894  GACGTC               GACGT^C
             3852  GACGTC              
AccI         2137  GTCGAC               GT^[AC][GT]AC
             3574  GTCTAC              
AccIII       3368  TCCGGA               T^CCGGA
             5436  TCCGGA              
AcsI          381  AAATTT               [GA]^AATT[CT]
             1759  AAATTT              
AocI         1047  CCTCAGG              CC^T[ACGT]AGG
             1366  CCTCAGG             
ApoI          381  AAATTT               [GA]^AATT[CT]
             1759  AAATTT              
BbvCI        4160  CCTCAGC              CCTCAGC(-5/-2)
              303  GCTGAGG             
BcgI         2203  GCAAACCTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2675  GCATGTTCTTCG        
BseAI        3368  TCCGGA               T^CCGGA
             5436  TCCGGA              
BspEI        3368  TCCGGA               T^CCGGA
             5436  TCCGGA              
BstAPI        201  GCATCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2594  GCAACGTGTGC         
Bsu36I       1047  CCTCAGG              CC^T[ACGT]AGG
             1366  CCTCAGG             
DrdI         3565  GACTACAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4372  GACGGGCAGGTC        
Eco81I       1047  CCTCAGG              CC^T[ACGT]AGG
             1366  CCTCAGG             
EcoNI        2556  CCTGAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3165  CCTGGGGGAGG         
HindIII      3350  AAGCTT               A^AGCTT
             4397  AAGCTT              
MroI         3368  TCCGGA               T^CCGGA
             5436  TCCGGA              
NcoI           50  CCATGG               C^CATGG
             3485  CCATGG              
SacI         3463  GAGCTC               GAGCT^C
             5463  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (  155 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        AspHI       
AspI        AvaI        AvaII       BalI        BamHI       
BanI        BanII       BbeI        BbsI        BbvI        
BciVI       BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssKI       
BssSI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DraIII      DsaI        EaeI        EarI        EcoO109I    
EcoO65I     EcoRII      EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
KasI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NsiI        NspI        PflFI       PflMI       
PleI        PpuMI       PshAI       PstI        PvuII       
RcaI        RsaI        SacII       SapI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SfoI        SmaI        SmlI        SphI        SspBI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI