##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AclI AflIII AhdI Alw44I ApaLI
AscI Asp700I AspEI BbrPI BciVI
BsaAI BsaBI BspLU11I BssHII Bst1107I
BstEII BstPI BstZ17I CpoI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
EcoT22I FseI KspI MamI MfeI
MluI MunI NdeI NotI NruI
NsiI PacI PaeR7I PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SexAI SnaBI SpeI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 6184 GGTACC G^GTACC
AccI 5253 GTAGAC GT^[AC][GT]AC
AccII 4603 CGCG CG^CG
AccIII 3156 TCCGGA T^CCGGA
AflII 4648 CTTAAG C^TTAAG
AgeI 3403 ACCGGT A^CCGGT
Aor51HI 2832 AGCGCT AGC^GCT
Asp718I 6184 GGTACC G^GTACC
BamHI 2841 GGATCC G^GATCC
BcgI 2259 CGATTGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2991 TGATCA T^GATCA
BfrI 4648 CTTAAG C^TTAAG
BseAI 3156 TCCGGA T^CCGGA
BsiEI 2744 CGGTCG CG[GA][CT]^CG
BsiWI 2637 CGTACG C^GTACG
BspEI 3156 TCCGGA T^CCGGA
BspHI 2160 TCATGA T^CATGA
BstBI 3311 TTCGAA TT^CGAA
BstUI 4603 CGCG CG^CG
DraI 4676 TTTAAA TTT^AAA
DraIII 3558 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1613 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2832 AGCGCT AGC^GCT
EcoRI 4902 GAATTC G^AATTC
FbaI 2991 TGATCA T^GATCA
HindIII 4651 AAGCTT A^AGCTT
HpaI 1513 GTTAAC GTT^AAC
KpnI 6184 GGTACC GGTAC^C
MroI 3156 TCCGGA T^CCGGA
MvnI 4603 CGCG CG^CG
NaeI 4353 GCCGGC GCC^GGC
NgoMIV 4353 GCCGGC G^CCGGC
NheI 3754 GCTAGC G^CTAGC
NspV 3311 TTCGAA TT^CGAA
PinAI 3403 ACCGGT A^CCGGT
RcaI 2160 TCATGA T^CATGA
SacI 2564 GAGCTC GAGCT^C
ScaI 4931 AGTACT AGT^ACT
SfiI 3331 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3311 TTCGAA TT^CGAA
SgrAI 2381 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1479 CCCGGG CCC^GGG
XmaCI 1479 CCCGGG C^CCGGG
XmaI 1479 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 953 GACGTC GACGT^C
2704 GACGTC
ApaI 4539 GGGCCC GGGCC^C
5878 GGGCCC
AviII 669 TGCGCA TGC^GCA
5270 TGCGCA
AvrII 1346 CCTAGG C^CTAGG
1457 CCTAGG
BlnI 1346 CCTAGG C^CTAGG
1457 CCTAGG
BsmBI 63 GAGACG CGTCTC(1/5)
1775 GAGACG
BsmI 3771 GCATTC GAATGC(1/-1)
4695 GCATTC
Bsp1407I 5192 TGTACA T^GTACA
6289 TGTACA
BspDI 3116 ATCGAT AT^CGAT
4590 ATCGAT
BsrBI 1870 CCGCTC CCGCTC(-3/-3)
2826 GAGCGG
BsrDI 3139 CATTGC GCAATG(2/0)
6047 GCAATG
BsrGI 5192 TGTACA T^GTACA
6289 TGTACA
BssSI 3149 CACGAG CACGAG(-5/-1)
1 CTCGTG
ClaI 3116 ATCGAT AT^CGAT
4590 ATCGAT
FspI 669 TGCGCA TGC^GCA
5270 TGCGCA
SapI 2339 GAAGAGC GCTCTTC(1/4)
303 GCTCTTC
SbfI 3334 CCTGCAGG CCTGCA^GG
3658 CCTGCAGG
SmlI 4648 CTTAAG C^T[CT][GA]AG
5384 CTCAAG
SphI 1761 GCATGC GCATG^C
1786 GCATGC
Sse8387I 3334 CCTGCAGG CCTGCA^GG
3658 CCTGCAGG
SspBI 5192 TGTACA T^GTACA
6289 TGTACA
SspI 5536 AATATT AAT^ATT
6246 AATATT
XcmI 1758 CCAGCATGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2909 CCAGTGCAGAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AseI
AsnI AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII