##################################
# NO CUT ENZYMES ( 31 enzymes) #
##################################
AatII AflII Aor51HI AscI AvrII
BamHI BciVI BfrI BlnI BsiWI
BspDI BspHI Bst1107I BstZ17I ClaI
CpoI DrdI Eco47III EcoRI EcoRV
HpaI NotI NruI PacI PaeR7I
PvuI RcaI RsrII SnaBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 3772 GGTACC G^GTACC
AclI 4000 AACGTT AA^CGTT
AgeI 3063 ACCGGT A^CCGGT
AhdI 4652 GACTATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 4305 ATTAAT AT^TAAT
AsnI 4305 ATTAAT AT^TAAT
Asp700I 5159 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3772 GGTACC G^GTACC
AspEI 4652 GACTATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 476 TGCGCA TGC^GCA
BbrPI 892 CACGTG CAC^GTG
BcgI 2076 CGACGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5898 TGATCA T^GATCA
BglII 1883 AGATCT A^GATCT
BsaBI 552 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2560 GAGACG CGTCTC(1/5)
Bsp1407I 5289 TGTACA T^GTACA
BsrGI 5289 TGTACA T^GTACA
BstBI 4493 TTCGAA TT^CGAA
BstXI 4903 CCAGCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 4652 GACTATACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 5127 ATGCAT ATGCA^T
FbaI 5898 TGATCA T^GATCA
FseI 375 GGCCGGCC GGCCGG^CC
FspI 476 TGCGCA TGC^GCA
HindIII 5186 AAGCTT A^AGCTT
KpnI 3772 GGTACC GGTAC^C
KspI 1747 CCGCGG CCGC^GG
MamI 552 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5018 CAATTG C^AATTG
MluI 1920 ACGCGT A^CGCGT
MunI 5018 CAATTG C^AATTG
NdeI 5116 CATATG CA^TATG
NsiI 5127 ATGCAT ATGCA^T
NspV 4493 TTCGAA TT^CGAA
PinAI 3063 ACCGGT A^CCGGT
PmaCI 892 CACGTG CAC^GTG
PmeI 5398 GTTTAAAC GTTT^AAAC
PmlI 892 CACGTG CAC^GTG
PshAI 2191 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4305 ATTAAT AT^TAAT
Psp1406I 4000 AACGTT AA^CGTT
SacII 1747 CCGCGG CCGC^GG
SalI 2532 GTCGAC G^TCGAC
SbfI 2574 CCTGCAGG CCTGCA^GG
ScaI 4109 AGTACT AGT^ACT
SexAI 1096 ACCTGGT A^CC[AT]GGT
SfuI 4493 TTCGAA TT^CGAA
SgrAI 2232 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 2574 CCTGCAGG CCTGCA^GG
SspBI 5289 TGTACA T^GTACA
XmnI 5159 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2532 GTCGAC GT^[AC][GT]AC
5368 GTAGAC
Alw44I 199 GTGCAC G^TGCAC
895 GTGCAC
ApaLI 199 GTGCAC G^TGCAC
895 GTGCAC
AspI 1549 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5976 GACCCAGTC
BbvCI 348 GCTGAGG CCTCAGC(-5/-2)
3345 GCTGAGG
BsaAI 892 CACGTG [CT]AC^GT[GA]
4063 TACGTG
BsmI 2794 GAATGC GAATGC(1/-1)
4533 GAATGC
BspLU11I 4039 ACATGT A^CATGT
5286 ACATGT
BstEII 2428 GGTCACC G^GT[ACGT]ACC
4855 GGTGACC
BstPI 2428 GGTCACC G^GT[ACGT]ACC
4855 GGTGACC
BstYI 1883 AGATCT [GA]^GATC[CT]
4994 GGATCT
EcoO65I 2428 GGTCACC G^GT[ACGT]ACC
4855 GGTGACC
HincII 1555 GTCAAC GT[CT]^[GA]AC
2532 GTCGAC
HindII 1555 GTCAAC GT[CT]^[GA]AC
2532 GTCGAC
MflI 1883 AGATCT [GA]^GATC[CT]
4994 GGATCT
NcoI 240 CCATGG C^CATGG
3904 CCATGG
PflFI 1549 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5976 GACCCAGTC
SapI 230 GCTCTTC GCTCTTC(1/4)
2867 GCTCTTC
SmlI 1189 CTCAAG C^T[CT][GA]AG
1618 CTCAAG
SpeI 5353 ACTAGT A^CTAGT
5970 ACTAGT
Tth111I 1549 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5976 GACCCAGTC
XbaI 2299 TCTAGA T^CTAGA
4030 TCTAGA
XhoII 1883 AGATCT [GA]^GATC[CT]
4994 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI
BsrI BssHII BssKI BssSI BstAPI
BstF5I BstNI BstUI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NheI NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SfoI
SmaI SphI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XmaCI XmaI