##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
Asp718I AviII AvrII BamHI BcgI
BglII BlnI BsiWI BsmBI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI FspI HindIII KpnI MluI
NotI NruI NspV PacI PinAI
PmeI Psp1406I PvuI RsrII SapI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 1074 TCCGGA T^CCGGA
AhdI 3417 GACTCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3417 GACTCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2439 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsaBI 1817 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1074 TCCGGA T^CCGGA
BsmI 3458 GCATTC GAATGC(1/-1)
Bsp1407I 3553 TGTACA T^GTACA
BspEI 1074 TCCGGA T^CCGGA
BspHI 1909 TCATGA T^CATGA
BsrGI 3553 TGTACA T^GTACA
BssSI 3935 CACGAG CACGAG(-5/-1)
BstAPI 3181 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2468 GGTTACC G^GT[ACGT]ACC
BstPI 2468 GGTTACC G^GT[ACGT]ACC
DrdI 4377 GACTACCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3417 GACTCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2468 GGTTACC G^GT[ACGT]ACC
EcoRV 2676 GATATC GAT^ATC
FseI 239 GGCCGGCC GGCCGG^CC
MamI 1817 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3746 CAATTG C^AATTG
MroI 1074 TCCGGA T^CCGGA
MunI 3746 CAATTG C^AATTG
NaeI 240 GCCGGC GCC^GGC
NdeI 1450 CATATG CA^TATG
NgoMIV 240 GCCGGC G^CCGGC
PaeR7I 4142 CTCGAG C^TCGAG
PflFI 2439 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2117 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1909 TCATGA T^CATGA
SacI 518 GAGCTC GAGCT^C
SalI 1008 GTCGAC G^TCGAC
SexAI 5150 ACCTGGT A^CC[AT]GGT
SspBI 3553 TGTACA T^GTACA
SwaI 5943 ATTTAAAT ATTT^AAAT
Tth111I 2439 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4867 TCTAGA T^CTAGA
XhoI 4142 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1008 GTCGAC GT^[AC][GT]AC
5300 GTAGAC
AflII 1264 CTTAAG C^TTAAG
2771 CTTAAG
AocI 2891 CCTGAGG CC^T[ACGT]AGG
5020 CCTCAGG
AseI 4308 ATTAAT AT^TAAT
4352 ATTAAT
AsnI 4308 ATTAAT AT^TAAT
4352 ATTAAT
BbeI 44 GGCGCC GGCGC^C
416 GGCGCC
BbvCI 2496 CCTCAGC CCTCAGC(-5/-2)
4575 GCTGAGG
BclI 684 TGATCA T^GATCA
3083 TGATCA
BfrI 1264 CTTAAG C^TTAAG
2771 CTTAAG
BglI 201 GCCCCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
262 GCCGTCGTGGC
BsaWI 1074 TCCGGA [AT]^CCGG[AT]
1689 ACCGGA
BsgI 3404 GTGCAG GTGCAG(16/14)
5164 GTGCAG
BsrBI 170 CCGCTC CCGCTC(-3/-3)
4069 CCGCTC
BsrFI 240 GCCGGC [GA]^CCGG[CT]
974 GCCGGT
BstYI 4195 GGATCT [GA]^GATC[CT]
5957 GGATCT
Bsu36I 2891 CCTGAGG CC^T[ACGT]AGG
5020 CCTCAGG
Cfr10I 240 GCCGGC [GA]^CCGG[CT]
974 GCCGGT
DraIII 3217 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
5450 CACAGGGTG
EagI 373 CGGCCG C^GGCCG
541 CGGCCG
EclXI 373 CGGCCG C^GGCCG
541 CGGCCG
Eco52I 373 CGGCCG C^GGCCG
541 CGGCCG
Eco81I 2891 CCTGAGG CC^T[ACGT]AGG
5020 CCTCAGG
EcoRI 1847 GAATTC G^AATTC
2794 GAATTC
FbaI 684 TGATCA T^GATCA
3083 TGATCA
HpaI 2842 GTTAAC GTT^AAC
4498 GTTAAC
KasI 44 GGCGCC G^GCGCC
416 GGCGCC
MflI 4195 GGATCT [GA]^GATC[CT]
5957 GGATCT
NarI 44 GGCGCC GG^CGCC
416 GGCGCC
NheI 2545 GCTAGC G^CTAGC
4897 GCTAGC
PshBI 4308 ATTAAT AT^TAAT
4352 ATTAAT
SbfI 4517 CCTGCAGG CCTGCA^GG
5538 CCTGCAGG
SfoI 44 GGCGCC GGC^GCC
416 GGCGCC
SphI 3922 GCATGC GCATG^C
3926 GCATGC
Sse8387I 4517 CCTGCAGG CCTGCA^GG
5538 CCTGCAGG
SspI 1725 AATATT AAT^ATT
5724 AATATT
XcmI 2596 CCATGGTTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4285 CCACTTGACTTCTGG
XhoII 4195 GGATCT [GA]^GATC[CT]
5957 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
Aor51HI ApaI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BciVI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XmnI