##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        Aor51HI     AscI        Asp718I     AspEI       
AspI        AviII       BbrPI       BcgI        BlpI        
Bpu1102I    BsaAI       BsaWI       BseAI       BsiWI       
BsmBI       BspEI       BssSI       BstBI       BstEII      
BstPI       CelII       CpoI        DraIII      DrdI        
Eam1105I    Eco47III    EcoO65I     FseI        FspI        
HpaI        KpnI        MluI        MroI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       Psp1406I    PvuI        RsrII       SbfI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    SwaI        Tth111I     XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcyI          173  GGCGCC               G[GA]^CG[CT]C
AocI         4275  CCTGAGG              CC^T[ACGT]AGG
ApaI           35  GGGCCC               GGGCC^C
AvaI         4914  CTCGGG               C^[CT]CG[GA]G
AvrII         290  CCTAGG               C^CTAGG
BamHI        2163  GGATCC               G^GATCC
BbeI          173  GGCGCC               GGCGC^C
BclI         1287  TGATCA               T^GATCA
BglII        5399  AGATCT               A^GATCT
BlnI          290  CCTAGG               C^CTAGG
BsaHI         173  GGCGCC               G[GA]^CG[CT]C
BsoBI        4914  CTCGGG               C^[CT]CG[GA]G
BspDI           4  ATCGAT               AT^CGAT
BspHI        1421  TCATGA               T^CATGA
BspMI        1460  GCAGGT               ACCTGC(4/8)
BsrBI         209  CCGCTC               CCGCTC(-3/-3)
BsrDI        2420  CATTGC               GCAATG(2/0)
BssHII         99  GCGCGC               G^CGCGC
BstAPI        839  GCAAAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        4773  CCAGCACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       4275  CCTGAGG              CC^T[ACGT]AGG
ClaI            4  ATCGAT               AT^CGAT
EarI         4091  CTCTTC               CTCTTC(1/4)
Eco81I       4275  CCTGAGG              CC^T[ACGT]AGG
EcoRI        4482  GAATTC               G^AATTC
EcoT22I      2147  ATGCAT               ATGCA^T
FbaI         1287  TGATCA               T^GATCA
Hin1I         173  GGCGCC               G[GA]^CG[CT]C
KasI          173  GGCGCC               G^GCGCC
Ksp632I      4091  CTCTTC               CTCTTC(1/4)
NaeI          269  GCCGGC               GCC^GGC
NarI          173  GGCGCC               GG^CGCC
NcoI         1143  CCATGG               C^CATGG
NgoMIV        269  GCCGGC               G^CCGGC
NsiI         2147  ATGCAT               ATGCA^T
PshAI        5013  GACTCTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         154  CAGCTG               CAG^CTG
RcaI         1421  TCATGA               T^CATGA
SacI         2897  GAGCTC               GAGCT^C
SalI           44  GTCGAC               G^TCGAC
SapI         4090  GCTCTTC              GCTCTTC(1/4)
SexAI        1909  ACCTGGT              A^CC[AT]GGT
SfoI          173  GGCGCC               GGC^GCC
SpeI         2023  ACTAGT               A^CTAGT
XcmI          667  CCAAAAAAGAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AflII        1196  CTTAAG               C^TTAAG
             2775  CTTAAG              
AflIII       2921  ACATGT               A^C[GA][CT]GT
             4044  ACATGT              
Alw44I       3571  GTGCAC               G^TGCAC
             4893  GTGCAC              
ApaLI        3571  GTGCAC               G^TGCAC
             4893  GTGCAC              
AvaII        1033  GGACC                G^G[AT]CC
             3301  GGTCC               
BalI          385  TGGCCA               TGG^CCA
             4832  TGGCCA              
BbvCI        4787  GCTGAGG              CCTCAGC(-5/-2)
             4922  GCTGAGG             
BciVI        2518  GGATAC               GTATCC(6/5)
             2580  GGATAC              
BfrI         1196  CTTAAG               C^TTAAG
             2775  CTTAAG              
BglI           28  GCCCGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             6017  GCCCTGTTGGC         
BsaBI         295  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2340  GATGAGGATC          
BseRI         110  CTCCTC               GAGGAG(10/8)
             4244  CTCCTC              
BsmFI         586  GGGAC                GGGAC(10/14)
              608  GGGAC               
BsmI         1870  GAATGC               GAATGC(1/-1)
             5752  GAATGC              
BspLU11I     2921  ACATGT               A^CATGT
             4044  ACATGT              
BsrFI         269  GCCGGC               [GA]^CCGG[CT]
             5083  ACCGGC              
Bst1107I     2222  GTATAC               GTA^TAC
             2260  GTATAC              
BstZ17I      2222  GTATAC               GTA^TAC
             2260  GTATAC              
Cfr10I        269  GCCGGC               [GA]^CCGG[CT]
             5083  ACCGGC              
DraII          34  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             4456  AGGCCCC             
EcoNI        2604  CCTATAGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3128  CCTCAGAAAGG         
EcoO109I       34  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             4456  AGGCCCC             
EcoRV        1744  GATATC               GAT^ATC
             5726  GATATC              
HaeII          87  GGCGCT               [GA]GCGC^[CT]
              173  GGCGCC              
HgaI          335  GACGC                GACGC(5/10)
             4255  GCGTC               
KspI           30  CCGCGG               CCGC^GG
              236  CCGCGG              
MamI          295  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2340  GATGAGGATC          
MluNI         385  TGGCCA               TGG^CCA
             4832  TGGCCA              
MscI          385  TGGCCA               TGG^CCA
             4832  TGGCCA              
NdeI         4060  CATATG               CA^TATG
             6262  CATATG              
PflMI         450  CCAAAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3410  CCATAGGGTGG         
SacII          30  CCGCGG               CCGC^GG
              236  CCGCGG              
ScaI         1256  AGTACT               AGT^ACT
             5759  AGTACT              
Van91I        450  CCAAAATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3410  CCATAGGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApoI        AseI        
AsnI        Asp700I     AspHI       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    Bsp1407I    BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DsaI        EaeI        EagI        EclXI       Eco52I      
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MfeI        MflI        MnlI        MseI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PleI        PshBI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XbaI        
XhoII       XmnI