##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AspI AviII BbrPI BcgI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiWI
BsmBI BspEI BssSI BstBI BstEII
BstPI CelII CpoI DraIII DrdI
Eam1105I Eco47III EcoO65I FseI FspI
HpaI KpnI MluI MroI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SbfI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 173 GGCGCC G[GA]^CG[CT]C
AocI 4275 CCTGAGG CC^T[ACGT]AGG
ApaI 35 GGGCCC GGGCC^C
AvaI 4914 CTCGGG C^[CT]CG[GA]G
AvrII 290 CCTAGG C^CTAGG
BamHI 2163 GGATCC G^GATCC
BbeI 173 GGCGCC GGCGC^C
BclI 1287 TGATCA T^GATCA
BglII 5399 AGATCT A^GATCT
BlnI 290 CCTAGG C^CTAGG
BsaHI 173 GGCGCC G[GA]^CG[CT]C
BsoBI 4914 CTCGGG C^[CT]CG[GA]G
BspDI 4 ATCGAT AT^CGAT
BspHI 1421 TCATGA T^CATGA
BspMI 1460 GCAGGT ACCTGC(4/8)
BsrBI 209 CCGCTC CCGCTC(-3/-3)
BsrDI 2420 CATTGC GCAATG(2/0)
BssHII 99 GCGCGC G^CGCGC
BstAPI 839 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 4773 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4275 CCTGAGG CC^T[ACGT]AGG
ClaI 4 ATCGAT AT^CGAT
EarI 4091 CTCTTC CTCTTC(1/4)
Eco81I 4275 CCTGAGG CC^T[ACGT]AGG
EcoRI 4482 GAATTC G^AATTC
EcoT22I 2147 ATGCAT ATGCA^T
FbaI 1287 TGATCA T^GATCA
Hin1I 173 GGCGCC G[GA]^CG[CT]C
KasI 173 GGCGCC G^GCGCC
Ksp632I 4091 CTCTTC CTCTTC(1/4)
NaeI 269 GCCGGC GCC^GGC
NarI 173 GGCGCC GG^CGCC
NcoI 1143 CCATGG C^CATGG
NgoMIV 269 GCCGGC G^CCGGC
NsiI 2147 ATGCAT ATGCA^T
PshAI 5013 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 154 CAGCTG CAG^CTG
RcaI 1421 TCATGA T^CATGA
SacI 2897 GAGCTC GAGCT^C
SalI 44 GTCGAC G^TCGAC
SapI 4090 GCTCTTC GCTCTTC(1/4)
SexAI 1909 ACCTGGT A^CC[AT]GGT
SfoI 173 GGCGCC GGC^GCC
SpeI 2023 ACTAGT A^CTAGT
XcmI 667 CCAAAAAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflII 1196 CTTAAG C^TTAAG
2775 CTTAAG
AflIII 2921 ACATGT A^C[GA][CT]GT
4044 ACATGT
Alw44I 3571 GTGCAC G^TGCAC
4893 GTGCAC
ApaLI 3571 GTGCAC G^TGCAC
4893 GTGCAC
AvaII 1033 GGACC G^G[AT]CC
3301 GGTCC
BalI 385 TGGCCA TGG^CCA
4832 TGGCCA
BbvCI 4787 GCTGAGG CCTCAGC(-5/-2)
4922 GCTGAGG
BciVI 2518 GGATAC GTATCC(6/5)
2580 GGATAC
BfrI 1196 CTTAAG C^TTAAG
2775 CTTAAG
BglI 28 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6017 GCCCTGTTGGC
BsaBI 295 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2340 GATGAGGATC
BseRI 110 CTCCTC GAGGAG(10/8)
4244 CTCCTC
BsmFI 586 GGGAC GGGAC(10/14)
608 GGGAC
BsmI 1870 GAATGC GAATGC(1/-1)
5752 GAATGC
BspLU11I 2921 ACATGT A^CATGT
4044 ACATGT
BsrFI 269 GCCGGC [GA]^CCGG[CT]
5083 ACCGGC
Bst1107I 2222 GTATAC GTA^TAC
2260 GTATAC
BstZ17I 2222 GTATAC GTA^TAC
2260 GTATAC
Cfr10I 269 GCCGGC [GA]^CCGG[CT]
5083 ACCGGC
DraII 34 GGGGCCC [GA]G^G[ACGT]CC[CT]
4456 AGGCCCC
EcoNI 2604 CCTATAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3128 CCTCAGAAAGG
EcoO109I 34 GGGGCCC [GA]G^G[ACGT]CC[CT]
4456 AGGCCCC
EcoRV 1744 GATATC GAT^ATC
5726 GATATC
HaeII 87 GGCGCT [GA]GCGC^[CT]
173 GGCGCC
HgaI 335 GACGC GACGC(5/10)
4255 GCGTC
KspI 30 CCGCGG CCGC^GG
236 CCGCGG
MamI 295 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2340 GATGAGGATC
MluNI 385 TGGCCA TGG^CCA
4832 TGGCCA
MscI 385 TGGCCA TGG^CCA
4832 TGGCCA
NdeI 4060 CATATG CA^TATG
6262 CATATG
PflMI 450 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3410 CCATAGGGTGG
SacII 30 CCGCGG CCGC^GG
236 CCGCGG
ScaI 1256 AGTACT AGT^ACT
5759 AGTACT
Van91I 450 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3410 CCATAGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EagI EclXI Eco52I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PshBI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII XmnI