################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Aor51HI AscI Asp718I AspEI AspI AviII BbrPI BcgI BlpI Bpu1102I BsaAI BsaWI BseAI BsiWI BsmBI BspEI BssSI BstBI BstEII BstPI CelII CpoI DraIII DrdI Eam1105I Eco47III EcoO65I FseI FspI HpaI KpnI MluI MroI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SbfI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcyI 173 GGCGCC G[GA]^CG[CT]C AocI 4275 CCTGAGG CC^T[ACGT]AGG ApaI 35 GGGCCC GGGCC^C AvaI 4914 CTCGGG C^[CT]CG[GA]G AvrII 290 CCTAGG C^CTAGG BamHI 2163 GGATCC G^GATCC BbeI 173 GGCGCC GGCGC^C BclI 1287 TGATCA T^GATCA BglII 5399 AGATCT A^GATCT BlnI 290 CCTAGG C^CTAGG BsaHI 173 GGCGCC G[GA]^CG[CT]C BsoBI 4914 CTCGGG C^[CT]CG[GA]G BspDI 4 ATCGAT AT^CGAT BspHI 1421 TCATGA T^CATGA BspMI 1460 GCAGGT ACCTGC(4/8) BsrBI 209 CCGCTC CCGCTC(-3/-3) BsrDI 2420 CATTGC GCAATG(2/0) BssHII 99 GCGCGC G^CGCGC BstAPI 839 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 4773 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 4275 CCTGAGG CC^T[ACGT]AGG ClaI 4 ATCGAT AT^CGAT EarI 4091 CTCTTC CTCTTC(1/4) Eco81I 4275 CCTGAGG CC^T[ACGT]AGG EcoRI 4482 GAATTC G^AATTC EcoT22I 2147 ATGCAT ATGCA^T FbaI 1287 TGATCA T^GATCA Hin1I 173 GGCGCC G[GA]^CG[CT]C KasI 173 GGCGCC G^GCGCC Ksp632I 4091 CTCTTC CTCTTC(1/4) NaeI 269 GCCGGC GCC^GGC NarI 173 GGCGCC GG^CGCC NcoI 1143 CCATGG C^CATGG NgoMIV 269 GCCGGC G^CCGGC NsiI 2147 ATGCAT ATGCA^T PshAI 5013 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 154 CAGCTG CAG^CTG RcaI 1421 TCATGA T^CATGA SacI 2897 GAGCTC GAGCT^C SalI 44 GTCGAC G^TCGAC SapI 4090 GCTCTTC GCTCTTC(1/4) SexAI 1909 ACCTGGT A^CC[AT]GGT SfoI 173 GGCGCC GGC^GCC SpeI 2023 ACTAGT A^CTAGT XcmI 667 CCAAAAAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AflII 1196 CTTAAG C^TTAAG 2775 CTTAAG AflIII 2921 ACATGT A^C[GA][CT]GT 4044 ACATGT Alw44I 3571 GTGCAC G^TGCAC 4893 GTGCAC ApaLI 3571 GTGCAC G^TGCAC 4893 GTGCAC AvaII 1033 GGACC G^G[AT]CC 3301 GGTCC BalI 385 TGGCCA TGG^CCA 4832 TGGCCA BbvCI 4787 GCTGAGG CCTCAGC(-5/-2) 4922 GCTGAGG BciVI 2518 GGATAC GTATCC(6/5) 2580 GGATAC BfrI 1196 CTTAAG C^TTAAG 2775 CTTAAG BglI 28 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 6017 GCCCTGTTGGC BsaBI 295 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2340 GATGAGGATC BseRI 110 CTCCTC GAGGAG(10/8) 4244 CTCCTC BsmFI 586 GGGAC GGGAC(10/14) 608 GGGAC BsmI 1870 GAATGC GAATGC(1/-1) 5752 GAATGC BspLU11I 2921 ACATGT A^CATGT 4044 ACATGT BsrFI 269 GCCGGC [GA]^CCGG[CT] 5083 ACCGGC Bst1107I 2222 GTATAC GTA^TAC 2260 GTATAC BstZ17I 2222 GTATAC GTA^TAC 2260 GTATAC Cfr10I 269 GCCGGC [GA]^CCGG[CT] 5083 ACCGGC DraII 34 GGGGCCC [GA]G^G[ACGT]CC[CT] 4456 AGGCCCC EcoNI 2604 CCTATAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3128 CCTCAGAAAGG EcoO109I 34 GGGGCCC [GA]G^G[ACGT]CC[CT] 4456 AGGCCCC EcoRV 1744 GATATC GAT^ATC 5726 GATATC HaeII 87 GGCGCT [GA]GCGC^[CT] 173 GGCGCC HgaI 335 GACGC GACGC(5/10) 4255 GCGTC KspI 30 CCGCGG CCGC^GG 236 CCGCGG MamI 295 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2340 GATGAGGATC MluNI 385 TGGCCA TGG^CCA 4832 TGGCCA MscI 385 TGGCCA TGG^CCA 4832 TGGCCA NdeI 4060 CATATG CA^TATG 6262 CATATG PflMI 450 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3410 CCATAGGGTGG SacII 30 CCGCGG CCGC^GG 236 CCGCGG ScaI 1256 AGTACT AGT^ACT 5759 AGTACT Van91I 450 CCAAAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3410 CCATAGGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AspHI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EagI EclXI Eco52I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI