################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbvCI BciVI BlnI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BsrDI BstEII BstPI ClaI Eam1105I EcoO65I FseI FspI KpnI KspI MluI MroI NheI NotI NruI PacI PinAI PshAI PshBI Psp1406I PvuI SacII SalI SexAI SgrAI SnaBI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccII 275 CGCG CG^CG AflII 3072 CTTAAG C^TTAAG Aor51HI 3282 AGCGCT AGC^GCT BbeI 1137 GGCGCC GGCGC^C BbrPI 1320 CACGTG CAC^GTG BcgI 1488 GCAGAAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3072 CTTAAG C^TTAAG BglII 725 AGATCT A^GATCT BlpI 2516 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2516 GCTCAGC GC^T[ACGT]AGC BsaAI 1320 CACGTG [CT]AC^GT[GA] BsiEI 1771 CGGCCG CG[GA][CT]^CG Bsp1407I 3419 TGTACA T^GTACA BspHI 2299 TCATGA T^CATGA BsrGI 3419 TGTACA T^GTACA BssHII 274 GCGCGC G^CGCGC Bst1107I 4581 GTATAC GTA^TAC BstAPI 2328 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 4373 TTCGAA TT^CGAA BstUI 275 CGCG CG^CG BstZ17I 4581 GTATAC GTA^TAC CelII 2516 GCTCAGC GC^T[ACGT]AGC CpoI 1022 CGGTCCG CG^G[AT]CCG DraIII 4030 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2768 GACTAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1771 CGGCCG C^GGCCG EclXI 1771 CGGCCG C^GGCCG Eco47III 3282 AGCGCT AGC^GCT Eco52I 1771 CGGCCG C^GGCCG EcoRI 2278 GAATTC G^AATTC EcoRV 3480 GATATC GAT^ATC HincII 4267 GTTAAC GT[CT]^[GA]AC HindII 4267 GTTAAC GT[CT]^[GA]AC HindIII 3769 AAGCTT A^AGCTT HpaI 4267 GTTAAC GTT^AAC KasI 1137 GGCGCC G^GCGCC MfeI 952 CAATTG C^AATTG MunI 952 CAATTG C^AATTG MvnI 275 CGCG CG^CG NaeI 3158 GCCGGC GCC^GGC NarI 1137 GGCGCC GG^CGCC NcoI 518 CCATGG C^CATGG NdeI 3723 CATATG CA^TATG NgoMIV 3158 GCCGGC G^CCGGC NspV 4373 TTCGAA TT^CGAA PmaCI 1320 CACGTG CAC^GTG PmeI 3608 GTTTAAAC GTTT^AAAC PmlI 1320 CACGTG CAC^GTG RcaI 2299 TCATGA T^CATGA RsrII 1022 CGGTCCG CG^G[AT]CCG SbfI 2894 CCTGCAGG CCTGCA^GG ScaI 4115 AGTACT AGT^ACT SfoI 1137 GGCGCC GGC^GCC SfuI 4373 TTCGAA TT^CGAA SpeI 972 ACTAGT A^CTAGT SphI 2337 GCATGC GCATG^C Sse8387I 2894 CCTGCAGG CCTGCA^GG SspBI 3419 TGTACA T^GTACA SspI 3916 AATATT AAT^ATT StuI 229 AGGCCT AGG^CCT SwaI 3259 ATTTAAAT ATTT^AAAT XbaI 915 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwNI 51 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2205 CAGGCGCTG AocI 1452 CCTCAGG CC^T[ACGT]AGG 1638 CCTGAGG ApaI 790 GGGCCC GGGCC^C 3196 GGGCCC AspI 171 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1018 GACCCGGTC BsaBI 474 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 720 GATGAAGATC BsaI 1465 GAGACC GGTCTC(1/5) 1474 GAGACC Bsu36I 1452 CCTCAGG CC^T[ACGT]AGG 1638 CCTGAGG Eco81I 1452 CCTCAGG CC^T[ACGT]AGG 1638 CCTGAGG MamI 474 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 720 GATGAAGATC PaeR7I 279 CTCGAG C^TCGAG 2508 CTCGAG PflFI 171 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1018 GACCCGGTC PflMI 518 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 794 CCACCAGCTGG PpuMI 1759 AGGACCT [GA]G^G[AT]CC[CT] 3120 GGGTCCC PvuII 798 CAGCTG CAG^CTG 3313 CAGCTG SacI 252 GAGCTC GAGCT^C 2247 GAGCTC SfiI 2048 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2868 GGCCGTGGGGGCC SmaI 61 CCCGGG CCC^GGG 1142 CCCGGG Tth111I 171 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1018 GACCCGGTC Van91I 518 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 794 CCACCAGCTGG XcmI 1591 CCAGGAACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2107 CCACTCACCTCATGG XhoI 279 CTCGAG C^TCGAG 2508 CTCGAG XmaCI 61 CCCGGG C^CCGGG 1142 CCCGGG XmaI 61 CCCGGG C^CCGGG 1142 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI