################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI AhdI Alw44I ApaLI AscI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbvCI BciVI BcnI BglI BlnI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstPI BstUI Cfr10I ClaI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FauI FseI FspI HapII HincII HindII HpaI HpaII KasI KpnI KspI MluI MroI MspI MvnI NaeI NarI NciI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 706 GTATAC GT^[AC][GT]AC AcyI 2667 GACGCC G[GA]^CG[CT]C AflII 1619 CTTAAG C^TTAAG Aor51HI 3656 AGCGCT AGC^GCT AvaI 1419 CTCGGG C^[CT]CG[GA]G BamHI 2840 GGATCC G^GATCC BfrI 1619 CTTAAG C^TTAAG BsaBI 5419 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2667 GACGCC G[GA]^CG[CT]C BsmI 1127 GCATTC GAATGC(1/-1) BsoBI 1419 CTCGGG C^[CT]CG[GA]G BspLU11I 5161 ACATGT A^CATGT BssSI 440 CTCGTG CACGAG(-5/-1) Bst1107I 706 GTATAC GTA^TAC BstZ17I 706 GTATAC GTA^TAC CpoI 1837 CGGACCG CG^G[AT]CCG DsaI 1347 CCATGG C^C[GA][CT]GG Eco47III 3656 AGCGCT AGC^GCT EcoRI 5041 GAATTC G^AATTC HaeII 3656 AGCGCT [GA]GCGC^[CT] Hin1I 2667 GACGCC G[GA]^CG[CT]C MamI 5419 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 1347 CCATGG C^CATGG NheI 5218 GCTAGC G^CTAGC PshAI 2331 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 1837 CGGACCG CG^G[AT]CCG SacI 2030 GAGCTC GAGCT^C SexAI 744 ACCAGGT A^CC[AT]GGT SpeI 784 ACTAGT A^CTAGT StuI 1356 AGGCCT AGG^CCT TaqI 1453 TCGA T^CGA TthHB8I 1453 TCGA T^CGA XcmI 1668 CCAAATATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 3005 ACACGT A^C[GA][CT]GT 5161 ACATGT AlwNI 1255 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1858 CAGGAGCTG ApaI 300 GGGCCC GGGCC^C 3063 GGGCCC AspHI 437 GTGCTC G[AT]GC[AT]^C 2030 GAGCTC BanI 4112 GGCACC G^G[CT][GA]CC 5505 GGTGCC BcgI 1812 CGAATGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 286 GCACACAATTCG BglII 994 AGATCT A^GATCT 2024 AGATCT BlpI 2045 GCTGAGC GC^T[ACGT]AGC 3125 GCTCAGC Bpu1102I 2045 GCTGAGC GC^T[ACGT]AGC 3125 GCTCAGC BsiHKAI 437 GTGCTC G[AT]GC[AT]^C 2030 GAGCTC BspMI 2200 ACCTGC ACCTGC(4/8) 2967 ACCTGC BsrDI 1231 CATTGC GCAATG(2/0) 3133 CATTGC BstAPI 4172 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4475 GCACTTTATGC CelII 2045 GCTGAGC GC^T[ACGT]AGC 3125 GCTCAGC DrdI 2422 GACCACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2966 GACCTGCCTGTC HgaI 2667 GACGC GACGC(5/10) 5222 GCGTC MaeII 2598 ACGT A^CGT 3007 ACGT MfeI 206 CAATTG C^AATTG 4993 CAATTG MspA1I 266 CAGCTG C[AC]G^C[GT]G 1196 CAGCTG MunI 206 CAATTG C^AATTG 4993 CAATTG NdeI 571 CATATG CA^TATG 5563 CATATG PvuII 266 CAGCTG CAG^CTG 1196 CAGCTG SapI 2261 GAAGAGC GCTCTTC(1/4) 2270 GAAGAGC SwaI 3241 ATTTAAAT ATTT^AAAT 4839 ATTTAAAT ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AocI ApoI AseI AsnI AvaII BalI BanII BbsI BbvI BclI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I EcoT22I FbaI Fnu4HI FokI HaeIII HhaI HinP1I HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII