##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AgeI        AhdI        Alw44I      ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BbeI        BbrPI       BbvCI       BciVI       
BcnI        BglI        BlnI        BsaAI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BsrBI       BsrFI       BsrGI       
BssHII      BstBI       BstEII      BstPI       BstUI       
Cfr10I      ClaI        DraIII      EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRV       FauI        
FseI        FspI        HapII       HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MluI        MroI        MspI        MvnI        NaeI        
NarI        NciI        NgoMIV      NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SspBI       Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI          706  GTATAC               GT^[AC][GT]AC
AcyI         2667  GACGCC               G[GA]^CG[CT]C
AflII        1619  CTTAAG               C^TTAAG
Aor51HI      3656  AGCGCT               AGC^GCT
AvaI         1419  CTCGGG               C^[CT]CG[GA]G
BamHI        2840  GGATCC               G^GATCC
BfrI         1619  CTTAAG               C^TTAAG
BsaBI        5419  GATTTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        2667  GACGCC               G[GA]^CG[CT]C
BsmI         1127  GCATTC               GAATGC(1/-1)
BsoBI        1419  CTCGGG               C^[CT]CG[GA]G
BspLU11I     5161  ACATGT               A^CATGT
BssSI         440  CTCGTG               CACGAG(-5/-1)
Bst1107I      706  GTATAC               GTA^TAC
BstZ17I       706  GTATAC               GTA^TAC
CpoI         1837  CGGACCG              CG^G[AT]CCG
DsaI         1347  CCATGG               C^C[GA][CT]GG
Eco47III     3656  AGCGCT               AGC^GCT
EcoRI        5041  GAATTC               G^AATTC
HaeII        3656  AGCGCT               [GA]GCGC^[CT]
Hin1I        2667  GACGCC               G[GA]^CG[CT]C
MamI         5419  GATTTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         1347  CCATGG               C^CATGG
NheI         5218  GCTAGC               G^CTAGC
PshAI        2331  GACAGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        1837  CGGACCG              CG^G[AT]CCG
SacI         2030  GAGCTC               GAGCT^C
SexAI         744  ACCAGGT              A^CC[AT]GGT
SpeI          784  ACTAGT               A^CTAGT
StuI         1356  AGGCCT               AGG^CCT
TaqI         1453  TCGA                 T^CGA
TthHB8I      1453  TCGA                 T^CGA
XcmI         1668  CCAAATATGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII       3005  ACACGT               A^C[GA][CT]GT
             5161  ACATGT              
AlwNI        1255  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1858  CAGGAGCTG           
ApaI          300  GGGCCC               GGGCC^C
             3063  GGGCCC              
AspHI         437  GTGCTC               G[AT]GC[AT]^C
             2030  GAGCTC              
BanI         4112  GGCACC               G^G[CT][GA]CC
             5505  GGTGCC              
BcgI         1812  CGAATGAAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              286  GCACACAATTCG        
BglII         994  AGATCT               A^GATCT
             2024  AGATCT              
BlpI         2045  GCTGAGC              GC^T[ACGT]AGC
             3125  GCTCAGC             
Bpu1102I     2045  GCTGAGC              GC^T[ACGT]AGC
             3125  GCTCAGC             
BsiHKAI       437  GTGCTC               G[AT]GC[AT]^C
             2030  GAGCTC              
BspMI        2200  ACCTGC               ACCTGC(4/8)
             2967  ACCTGC              
BsrDI        1231  CATTGC               GCAATG(2/0)
             3133  CATTGC              
BstAPI       4172  GCAGAGGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             4475  GCACTTTATGC         
CelII        2045  GCTGAGC              GC^T[ACGT]AGC
             3125  GCTCAGC             
DrdI         2422  GACCACACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2966  GACCTGCCTGTC        
HgaI         2667  GACGC                GACGC(5/10)
             5222  GCGTC               
MaeII        2598  ACGT                 A^CGT
             3007  ACGT                
MfeI          206  CAATTG               C^AATTG
             4993  CAATTG              
MspA1I        266  CAGCTG               C[AC]G^C[GT]G
             1196  CAGCTG              
MunI          206  CAATTG               C^AATTG
             4993  CAATTG              
NdeI          571  CATATG               CA^TATG
             5563  CATATG              
PvuII         266  CAGCTG               CAG^CTG
             1196  CAGCTG              
SapI         2261  GAAGAGC              GCTCTTC(1/4)
             2270  GAAGAGC             
SwaI         3241  ATTTAAAT             ATTT^AAAT
             4839  ATTTAAAT            


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        AseI        AsnI        AvaII       
BalI        BanII       BbsI        BbvI        BclI        
BfaI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
EcoT22I     FbaI        Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HindIII     HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PleI        
PpuMI       PshBI       PstI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII