##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccI AclI AflII Aor51HI AscI
AseI AsnI BbeI BfrI BlpI
Bpu1102I BsaBI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstZ17I
CelII ClaI DraI Eco47III EcoRI
EcoRV FseI HpaI KasI MamI
MfeI MluI MunI NarI NheI
NotI NruI NspI NspV PacI
PmeI PshAI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 3804 GACGTC GACGT^C
AccIII 1263 TCCGGA T^CCGGA
AgeI 4478 ACCGGT A^CCGGT
AhdI 3334 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 347 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3334 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4835 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3653 TGCGCA TGC^GCA
BbrPI 2311 CACGTG CAC^GTG
BglII 864 AGATCT A^GATCT
BsaI 4106 GAGACC GGTCTC(1/5)
BseAI 1263 TCCGGA T^CCGGA
BsiWI 479 CGTACG C^GTACG
BsmBI 2348 GAGACG CGTCTC(1/5)
BsmI 3058 GCATTC GAATGC(1/-1)
BspEI 1263 TCCGGA T^CCGGA
BssHII 267 GCGCGC G^CGCGC
BstAPI 2420 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1790 GGTCACC G^GT[ACGT]ACC
BstPI 1790 GGTCACC G^GT[ACGT]ACC
CpoI 4435 CGGACCG CG^G[AT]CCG
Eam1105I 3334 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1790 GGTCACC G^GT[ACGT]ACC
EcoT22I 3772 ATGCAT ATGCA^T
FspI 3653 TGCGCA TGC^GCA
HindIII 1289 AAGCTT A^AGCTT
MroI 1263 TCCGGA T^CCGGA
NsiI 3772 ATGCAT ATGCA^T
PflFI 4835 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4478 ACCGGT A^CCGGT
PmaCI 2311 CACGTG CAC^GTG
PmlI 2311 CACGTG CAC^GTG
RsrII 4435 CGGACCG CG^G[AT]CCG
SfiI 1437 GGCCTTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3415 ACTAGT A^CTAGT
SspI 4968 AATATT AAT^ATT
Tth111I 4835 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 326 TCTAGA T^CTAGA
XcmI 309 CCAGGTGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 347 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1357 GGTACC G^GTACC
3605 GGTACC
AcyI 3238 GGCGTC G[GA]^CG[CT]C
3804 GACGTC
AflIII 3297 ACACGT A^C[GA][CT]GT
3563 ACGTGT
Alw44I 2077 GTGCAC G^TGCAC
2418 GTGCAC
ApaLI 2077 GTGCAC G^TGCAC
2418 GTGCAC
Asp718I 1357 GGTACC G^GTACC
3605 GGTACC
AvrII 339 CCTAGG C^CTAGG
1378 CCTAGG
BcgI 4265 CGAGGAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2492 GCAAGGGGCTCG
BciVI 539 GGATAC GTATCC(6/5)
4525 GTATCC
BlnI 339 CCTAGG C^CTAGG
1378 CCTAGG
BsaAI 2311 CACGTG [CT]AC^GT[GA]
3562 TACGTG
BsaHI 3238 GGCGTC G[GA]^CG[CT]C
3804 GACGTC
BsrDI 1809 CATTGC GCAATG(2/0)
3896 GCAATG
BssSI 1222 CACGAG CACGAG(-5/-1)
4983 CTCGTG
DrdI 2127 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2734 GACTACATGGTC
EagI 272 CGGCCG C^GGCCG
761 CGGCCG
EclXI 272 CGGCCG C^GGCCG
761 CGGCCG
Eco52I 272 CGGCCG C^GGCCG
761 CGGCCG
EcoNI 412 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1867 CCTGAGGCAGG
Hin1I 3238 GGCGTC G[GA]^CG[CT]C
3804 GACGTC
HincII 4319 GTCAAC GT[CT]^[GA]AC
4680 GTCAAC
HindII 4319 GTCAAC GT[CT]^[GA]AC
4680 GTCAAC
KpnI 1357 GGTACC GGTAC^C
3605 GGTACC
KspI 143 CCGCGG CCGC^GG
3496 CCGCGG
MseI 433 TTAA T^TAA
1709 TTAA
NdeI 705 CATATG CA^TATG
1153 CATATG
PaeR7I 3389 CTCGAG C^TCGAG
3979 CTCGAG
SacI 4760 GAGCTC GAGCT^C
4853 GAGCTC
SacII 143 CCGCGG CCGC^GG
3496 CCGCGG
SexAI 2195 ACCAGGT A^CC[AT]GGT
4007 ACCAGGT
SmaI 1346 CCCGGG CCC^GGG
1970 CCCGGG
StuI 3463 AGGCCT AGG^CCT
4328 AGGCCT
Tru9I 433 TTAA T^TAA
1709 TTAA
XhoI 3389 CTCGAG C^TCGAG
3979 CTCGAG
XmaCI 1346 CCCGGG C^CCGGG
1970 CCCGGG
XmaI 1346 CCCGGG C^CCGGG
1970 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII