##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AspI
AvrII BbrPI BciVI BlnI BsaAI
BsiWI BspDI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI HpaI KpnI KspI MfeI
MluI MunI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflII 4392 CTTAAG C^TTAAG
AflIII 2845 ACATGT A^C[GA][CT]GT
Alw44I 1301 GTGCAC G^TGCAC
ApaLI 1301 GTGCAC G^TGCAC
AviII 3895 TGCGCA TGC^GCA
BbeI 4894 GGCGCC GGCGC^C
BcgI 3749 GCAGACTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4392 CTTAAG C^TTAAG
BglI 4309 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3400 AGATCT A^GATCT
BsiEI 1478 CGACCG CG[GA][CT]^CG
BsmBI 131 GAGACG CGTCTC(1/5)
BspHI 3625 TCATGA T^CATGA
BspLU11I 2845 ACATGT A^CATGT
BsrBI 3813 GAGCGG CCGCTC(-3/-3)
BstEII 808 GGTTACC G^GT[ACGT]ACC
BstPI 808 GGTTACC G^GT[ACGT]ACC
DraIII 3475 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 808 GGTTACC G^GT[ACGT]ACC
EcoRI 1107 GAATTC G^AATTC
FspI 3895 TGCGCA TGC^GCA
HaeII 4894 GGCGCC [GA]GCGC^[CT]
HindIII 1725 AAGCTT A^AGCTT
KasI 4894 GGCGCC G^GCGCC
NarI 4894 GGCGCC GG^CGCC
NheI 2088 GCTAGC G^CTAGC
PshAI 944 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3625 TCATGA T^CATGA
SfoI 4894 GGCGCC GGC^GCC
SmaI 1363 CCCGGG CCC^GGG
SspI 237 AATATT AAT^ATT
XbaI 1148 TCTAGA T^CTAGA
XmaCI 1363 CCCGGG C^CCGGG
XmaI 1363 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 780 GTAGAC GT^[AC][GT]AC
4188 GTCTAC
AccII 1437 CGCG CG^CG
4744 CGCG
AcyI 133 GACGCC G[GA]^CG[CT]C
4894 GGCGCC
AhdI 4377 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5205 GACTTACTGTC
AocI 2883 CCTGAGG CC^T[ACGT]AGG
2952 CCTGAGG
AspEI 4377 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5205 GACTTACTGTC
BclI 3580 TGATCA T^GATCA
4330 TGATCA
BsaBI 623 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1712 GATGTACATC
BsaHI 133 GACGCC G[GA]^CG[CT]C
4894 GGCGCC
BsrDI 5086 CATTGC GCAATG(2/0)
3560 GCAATG
BstUI 1437 CGCG CG^CG
4744 CGCG
Bsu36I 2883 CCTGAGG CC^T[ACGT]AGG
2952 CCTGAGG
DraI 371 TTTAAA TTT^AAA
819 TTTAAA
DrdI 37 GACTGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4179 GACTCAGAGGTC
Eam1105I 4377 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5205 GACTTACTGTC
Eco81I 2883 CCTGAGG CC^T[ACGT]AGG
2952 CCTGAGG
FbaI 3580 TGATCA T^GATCA
4330 TGATCA
HgaI 133 GACGC GACGC(5/10)
4465 GCGTC
Hin1I 133 GACGCC G[GA]^CG[CT]C
4894 GGCGCC
MamI 623 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1712 GATGTACATC
MvnI 1437 CGCG CG^CG
4744 CGCG
NaeI 4603 GCCGGC GCC^GGC
6097 GCCGGC
NgoMIV 4603 GCCGGC G^CCGGC
6097 GCCGGC
SexAI 956 ACCAGGT A^CC[AT]GGT
3943 ACCAGGT
SphI 1015 GCATGC GCATG^C
1681 GCATGC
StuI 2150 AGGCCT AGG^CCT
2396 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspEI BspMI BsrFI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI