##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI ApaI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BcgI BlnI BlpI
Bpu1102I BsmBI BspDI BspLU11I BspMI
BsrBI BssHII Bst1107I BstEII BstPI
BstZ17I CelII ClaI CpoI DraIII
DrdI Eco47III EcoO65I FspI HpaI
KasI KpnI MluI NarI NdeI
NheI NotI NruI PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SapI SfoI SgrAI SnaBI
SpeI SwaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1407 GACGTC GACGT^C
AccIII 2135 TCCGGA T^CCGGA
AflII 3041 CTTAAG C^TTAAG
AflIII 3842 ACGTGT A^C[GA][CT]GT
AhdI 3253 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3253 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 4132 GGATCC G^GATCC
BbrPI 1953 CACGTG CAC^GTG
BbvCI 60 CCTCAGC CCTCAGC(-5/-2)
BfrI 3041 CTTAAG C^TTAAG
BglI 3207 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4493 AGATCT A^GATCT
BsaBI 11 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 594 GAGACC GGTCTC(1/5)
BsaWI 2135 TCCGGA [AT]^CCGG[AT]
BseAI 2135 TCCGGA T^CCGGA
BsiEI 3967 CGGCCG CG[GA][CT]^CG
BsiWI 2380 CGTACG C^GTACG
BspEI 2135 TCCGGA T^CCGGA
BsrFI 1836 GCCGGC [GA]^CCGG[CT]
BssSI 2970 CTCGTG CACGAG(-5/-1)
BstBI 2013 TTCGAA TT^CGAA
Cfr10I 1836 GCCGGC [GA]^CCGG[CT]
EagI 3967 CGGCCG C^GGCCG
Eam1105I 3253 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3967 CGGCCG C^GGCCG
Eco52I 3967 CGGCCG C^GGCCG
EcoT22I 4084 ATGCAT ATGCA^T
FseI 1835 GGCCGGCC GGCCGG^CC
HaeII 2109 GGCGCT [GA]GCGC^[CT]
HgaI 4146 GCGTC GACGC(5/10)
KspI 2809 CCGCGG CCGC^GG
MamI 11 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2135 TCCGGA T^CCGGA
NaeI 1836 GCCGGC GCC^GGC
NgoMIV 1836 GCCGGC G^CCGGC
NsiI 4084 ATGCAT ATGCA^T
NspV 2013 TTCGAA TT^CGAA
PaeR7I 2902 CTCGAG C^TCGAG
PflMI 3377 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1953 CACGTG CAC^GTG
PmlI 1953 CACGTG CAC^GTG
PpuMI 4160 AGGACCT [GA]G^G[AT]CC[CT]
PstI 3210 CTGCAG CTGCA^G
SacII 2809 CCGCGG CCGC^GG
SbfI 3209 CCTGCAGG CCTGCA^GG
SfiI 3206 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2013 TTCGAA TT^CGAA
SmaI 1475 CCCGGG CCC^GGG
Sse8387I 3209 CCTGCAGG CCTGCA^GG
StuI 4272 AGGCCT AGG^CCT
Van91I 3377 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4505 TCTAGA T^CTAGA
XhoI 2902 CTCGAG C^TCGAG
XmaCI 1475 CCCGGG C^CCGGG
XmaI 1475 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 1407 GACGTC G[GA]^CG[CT]C
4145 GGCGTC
AspI 2360 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3872 GACAGAGTC
AvaII 4161 GGACC G^G[AT]CC
4877 GGACC
BalI 1120 TGGCCA TGG^CCA
3693 TGGCCA
BclI 317 TGATCA T^GATCA
1736 TGATCA
BsaAI 1953 CACGTG [CT]AC^GT[GA]
2382 TACGTG
BsaHI 1407 GACGTC G[GA]^CG[CT]C
4145 GGCGTC
BsmI 3421 GCATTC GAATGC(1/-1)
4837 GCATTC
Bsp1407I 1328 TGTACA T^GTACA
3327 TGTACA
BsrDI 745 CATTGC GCAATG(2/0)
3244 GCAATG
BsrGI 1328 TGTACA T^GTACA
3327 TGTACA
BstYI 4132 GGATCC [GA]^GATC[CT]
4493 AGATCT
DraI 5198 TTTAAA TTT^AAA
5299 TTTAAA
EcoNI 990 CCTCACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2898 CCTGCTCGAGG
EcoRV 560 GATATC GAT^ATC
2878 GATATC
FbaI 317 TGATCA T^GATCA
1736 TGATCA
Hin1I 1407 GACGTC G[GA]^CG[CT]C
4145 GGCGTC
HincII 2601 GTCGAC GT[CT]^[GA]AC
3338 GTCGAC
HindII 2601 GTCGAC GT[CT]^[GA]AC
3338 GTCGAC
HindIII 2348 AAGCTT A^AGCTT
3524 AAGCTT
MfeI 735 CAATTG C^AATTG
951 CAATTG
MflI 4132 GGATCC [GA]^GATC[CT]
4493 AGATCT
MluNI 1120 TGGCCA TGG^CCA
3693 TGGCCA
MscI 1120 TGGCCA TGG^CCA
3693 TGGCCA
MslI 1728 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3429 CACGAAAATG
MunI 735 CAATTG C^AATTG
951 CAATTG
PflFI 2360 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3872 GACAGAGTC
PvuII 4060 CAGCTG CAG^CTG
4607 CAGCTG
SacI 3394 GAGCTC GAGCT^C
4214 GAGCTC
SalI 2601 GTCGAC G^TCGAC
3338 GTCGAC
SexAI 2994 ACCAGGT A^CC[AT]GGT
4917 ACCAGGT
SspBI 1328 TGTACA T^GTACA
3327 TGTACA
Tth111I 2360 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3872 GACAGAGTC
XhoII 4132 GGATCC [GA]^GATC[CT]
4493 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI ApoI Asp700I AspHI AvaI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmnI