##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
ApaI AscI AspEI BamHI BciVI
BfrI BseAI BsiEI BsmBI BspEI
BspHI BstBI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoRV FseI
HpaI MroI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
PshAI PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SfiI
SfuI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 419 GGTACC G^GTACC
AclI 5244 AACGTT AA^CGTT
Aor51HI 1092 AGCGCT AGC^GCT
Asp718I 419 GGTACC G^GTACC
AviII 2034 TGCGCA TGC^GCA
BlpI 548 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 548 GCTGAGC GC^T[ACGT]AGC
BsaBI 2167 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2851 ACCGGA [AT]^CCGG[AT]
BsiWI 301 CGTACG C^GTACG
BspDI 2631 ATCGAT AT^CGAT
BstEII 331 GGTGACC G^GT[ACGT]ACC
BstPI 331 GGTGACC G^GT[ACGT]ACC
CelII 548 GCTGAGC GC^T[ACGT]AGC
ClaI 2631 ATCGAT AT^CGAT
Eco47III 1092 AGCGCT AGC^GCT
EcoO65I 331 GGTGACC G^GT[ACGT]ACC
EcoRI 4792 GAATTC G^AATTC
EcoT22I 942 ATGCAT ATGCA^T
FspI 2034 TGCGCA TGC^GCA
KpnI 419 GGTACC GGTAC^C
MamI 2167 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2089 ACGCGT A^CGCGT
NcoI 1470 CCATGG C^CATGG
NsiI 942 ATGCAT ATGCA^T
PaeR7I 4465 CTCGAG C^TCGAG
Psp1406I 5244 AACGTT AA^CGTT
SgrAI 99 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 4519 TACGTA TAC^GTA
StuI 2004 AGGCCT AGG^CCT
XcmI 1573 CCAGCAGTCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4465 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
Alw44I 713 GTGCAC G^TGCAC
1106 GTGCAC
AocI 4118 CCTAAGG CC^T[ACGT]AGG
4752 CCTAAGG
ApaLI 713 GTGCAC G^TGCAC
1106 GTGCAC
Asp700I 1540 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4025 GAATGATTTC
AspI 3221 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4676 GACGTAGTC
AvrII 1414 CCTAGG C^CTAGG
2176 CCTAGG
BbrPI 740 CACGTG CAC^GTG
4735 CACGTG
BglI 346 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
649 GCCAGGGTGGC
BlnI 1414 CCTAGG C^CTAGG
2176 CCTAGG
BsaI 772 GAGACC GGTCTC(1/5)
1335 GAGACC
Bsp1407I 5315 TGTACA T^GTACA
5366 TGTACA
BspLU11I 4301 ACATGT A^CATGT
5085 ACATGT
BspMI 1758 ACCTGC ACCTGC(4/8)
4050 ACCTGC
BsrBI 3839 CCGCTC CCGCTC(-3/-3)
415 GAGCGG
BsrDI 4428 CATTGC GCAATG(2/0)
2063 GCAATG
BsrGI 5315 TGTACA T^GTACA
5366 TGTACA
Bst1107I 3153 GTATAC GTA^TAC
4273 GTATAC
BstXI 4191 CCAGTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4761 CCAGCTCGCTGG
BstZ17I 3153 GTATAC GTA^TAC
4273 GTATAC
Bsu36I 4118 CCTAAGG CC^T[ACGT]AGG
4752 CCTAAGG
Eco81I 4118 CCTAAGG CC^T[ACGT]AGG
4752 CCTAAGG
KspI 15 CCGCGG CCGC^GG
222 CCGCGG
MfeI 1346 CAATTG C^AATTG
4324 CAATTG
MunI 1346 CAATTG C^AATTG
4324 CAATTG
PflFI 3221 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4676 GACGTAGTC
PmaCI 740 CACGTG CAC^GTG
4735 CACGTG
PmlI 740 CACGTG CAC^GTG
4735 CACGTG
SacII 15 CCGCGG CCGC^GG
222 CCGCGG
SexAI 3594 ACCTGGT A^CC[AT]GGT
4397 ACCTGGT
SmaI 195 CCCGGG CCC^GGG
236 CCCGGG
SpeI 1393 ACTAGT A^CTAGT
2695 ACTAGT
SphI 975 GCATGC GCATG^C
1015 GCATGC
SspBI 5315 TGTACA T^GTACA
5366 TGTACA
SspI 707 AATATT AAT^ATT
2965 AATATT
Tth111I 3221 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4676 GACGTAGTC
XbaI 3375 TCTAGA T^CTAGA
3495 TCTAGA
XmaCI 195 CCCGGG C^CCGGG
236 CCCGGG
XmaI 195 CCCGGG C^CCGGG
236 CCCGGG
XmnI 1540 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4025 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AseI AsnI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcgI BclI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssHII BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII