##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AccIII AcsI AflII AhdI ApoI
AscI AspEI AviII BamHI BcgI
BfrI BglII BseAI BsmBI BsmI
Bsp1407I BspDI BspEI BsrGI Bst1107I
BstBI BstZ17I ClaI Eam1105I EcoRI
EcoRV EcoT22I FspI HpaI MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PshAI PvuI SfuI SnaBI
SpeI SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1742 GACGTC GACGT^C
AclI 1526 AACGTT AA^CGTT
AflIII 2088 ACATGT A^C[GA][CT]GT
AgeI 4467 ACCGGT A^CCGGT
Alw44I 1441 GTGCAC G^TGCAC
AocI 5115 CCTGAGG CC^T[ACGT]AGG
ApaLI 1441 GTGCAC G^TGCAC
AseI 5290 ATTAAT AT^TAAT
AsnI 5290 ATTAAT AT^TAAT
AspI 3959 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 139 CCTAGG C^CTAGG
BbrPI 3165 CACGTG CAC^GTG
BciVI 3679 GGATAC GTATCC(6/5)
BclI 3145 TGATCA T^GATCA
BlnI 139 CCTAGG C^CTAGG
BlpI 3617 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3617 GCTAAGC GC^T[ACGT]AGC
BsaBI 2609 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1542 GAGACC GGTCTC(1/5)
BsiEI 4307 CGGCCG CG[GA][CT]^CG
BsiWI 223 CGTACG C^GTACG
BspHI 3028 TCATGA T^CATGA
BspLU11I 2088 ACATGT A^CATGT
BssHII 99 GCGCGC G^CGCGC
Bsu36I 5115 CCTGAGG CC^T[ACGT]AGG
CelII 3617 GCTAAGC GC^T[ACGT]AGC
CpoI 57 CGGTCCG CG^G[AT]CCG
EagI 4307 CGGCCG C^GGCCG
EclXI 4307 CGGCCG C^GGCCG
Eco52I 4307 CGGCCG C^GGCCG
Eco81I 5115 CCTGAGG CC^T[ACGT]AGG
FbaI 3145 TGATCA T^GATCA
HindIII 4244 AAGCTT A^AGCTT
MamI 2609 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 163 CTCGAG C^TCGAG
PflFI 3959 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4467 ACCGGT A^CCGGT
PmaCI 3165 CACGTG CAC^GTG
PmeI 4259 GTTTAAAC GTTT^AAAC
PmlI 3165 CACGTG CAC^GTG
PshBI 5290 ATTAAT AT^TAAT
Psp1406I 1526 AACGTT AA^CGTT
RcaI 3028 TCATGA T^CATGA
RsrII 57 CGGTCCG CG^G[AT]CCG
SacI 4698 GAGCTC GAGCT^C
SalI 1490 GTCGAC G^TCGAC
SbfI 2333 CCTGCAGG CCTGCA^GG
ScaI 4179 AGTACT AGT^ACT
SfiI 357 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2333 CCTGCAGG CCTGCA^GG
SspI 5287 AATATT AAT^ATT
Tth111I 3959 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 163 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Acc65I 2023 GGTACC G^GTACC
4637 GGTACC
AccI 1490 GTCGAC GT^[AC][GT]AC
1758 GTCTAC
Asp718I 2023 GGTACC G^GTACC
4637 GGTACC
BbvCI 145 CCTCAGC CCTCAGC(-5/-2)
2 GCTGAGG
BssSI 1584 CACGAG CACGAG(-5/-1)
3742 CACGAG
BstXI 208 CCATGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2783 CCAAGGCATTGG
BstYI 940 AGATCC [GA]^GATC[CT]
1840 AGATCC
FseI 3099 GGCCGGCC GGCCGG^CC
3816 GGCCGGCC
HincII 1490 GTCGAC GT[CT]^[GA]AC
3000 GTCAAC
HindII 1490 GTCGAC GT[CT]^[GA]AC
3000 GTCAAC
KpnI 2023 GGTACC GGTAC^C
4637 GGTACC
MflI 940 AGATCC [GA]^GATC[CT]
1840 AGATCC
SapI 3612 GAAGAGC GCTCTTC(1/4)
2291 GCTCTTC
SexAI 2647 ACCTGGT A^CC[AT]GGT
3199 ACCTGGT
SgrAI 2099 CGCCGGTG C[GA]^CCGG[CT]G
4466 CACCGGTG
SphI 1951 GCATGC GCATG^C
5076 GCATGC
XhoII 940 AGATCC [GA]^GATC[CT]
1840 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XmaCI XmaI XmnI