##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AccIII      AclI        AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AspEI       
AspI        AviII       BseAI       BsiWI       BsmBI       
BspEI       BssHII      BstBI       BstEII      BstPI       
CpoI        DraIII      Eam1105I    Eco47III    EcoNI       
EcoO65I     EcoRI       FseI        FspI        HpaI        
MfeI        MluI        MroI        MunI        NdeI        
NheI        NotI        NruI        NspV        PflFI       
PinAI       PmeI        PshAI       Psp1406I    PvuI        
RsrII       SalI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Tth111I     


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        2991  GACGTC               GACGT^C
Acc65I       5484  GGTACC               G^GTACC
AccI         5841  GTATAC               GT^[AC][GT]AC
AocI         2285  CCTGAGG              CC^T[ACGT]AGG
Asp718I      5484  GGTACC               G^GTACC
BamHI         825  GGATCC               G^GATCC
BbeI          265  GGCGCC               GGCGC^C
BcgI         3196  GCACACAAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2212  GGATAC               GTATCC(6/5)
BlpI         3782  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3782  GCTCAGC              GC^T[ACGT]AGC
BsaBI        2844  GATCGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        4113  ACCGGA               [AT]^CCGG[AT]
BspDI        2845  ATCGAT               AT^CGAT
BsrDI        1733  GCAATG               GCAATG(2/0)
BsrFI          99  GCCGGC               [GA]^CCGG[CT]
Bst1107I     5841  GTATAC               GTA^TAC
BstXI        1283  CCATCCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      5841  GTATAC               GTA^TAC
Bsu36I       2285  CCTGAGG              CC^T[ACGT]AGG
CelII        3782  GCTCAGC              GC^T[ACGT]AGC
Cfr10I         99  GCCGGC               [GA]^CCGG[CT]
ClaI         2845  ATCGAT               AT^CGAT
DrdI         4034  GACTGGGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2285  CCTGAGG              CC^T[ACGT]AGG
HincII       4602  GTTGAC               GT[CT]^[GA]AC
HindII       4602  GTTGAC               GT[CT]^[GA]AC
KasI          265  GGCGCC               G^GCGCC
KpnI         5484  GGTACC               GGTAC^C
MamI         2844  GATCGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI           99  GCCGGC               GCC^GGC
NarI          265  GGCGCC               GG^CGCC
NcoI         2274  CCATGG               C^CATGG
NgoMIV         99  GCCGGC               G^CCGGC
PacI         2261  TTAATTAA             TTAAT^TAA
PaeR7I       3426  CTCGAG               C^TCGAG
PflMI        3877  CCACTTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI         3029  GAAGAGC              GCTCTTC(1/4)
SbfI         5077  CCTGCAGG             CCTGCA^GG
SfoI          265  GGCGCC               GGC^GCC
Sse8387I     5077  CCTGCAGG             CCTGCA^GG
StuI         5074  AGGCCT               AGG^CCT
SwaI         5540  ATTTAAAT             ATTT^AAAT
Van91I       3877  CCACTTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         3654  CCAAGACTCGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         3426  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

Asp700I      1068  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2708  GAAACTATTC          
AvrII          40  CCTAGG               C^CTAGG
             3966  CCTAGG              
BalI         3666  TGGCCA               TGG^CCA
             5749  TGGCCA              
BbrPI         578  CACGTG               CAC^GTG
             5372  CACGTG              
BbvCI        2624  CCTCAGC              CCTCAGC(-5/-2)
             2207  GCTGAGG             
BlnI           40  CCTAGG               C^CTAGG
             3966  CCTAGG              
BpmI          135  CTGGAG               CTGGAG(16/14)
             2028  CTGGAG              
BsaAI         578  CACGTG               [CT]AC^GT[GA]
             5372  CACGTG              
BseRI          32  GAGGAG               GAGGAG(10/8)
             1481  GAGGAG              
BsgI         3744  CTGCAC               GTGCAG(16/14)
              843  GTGCAG              
BsiEI         101  CGGCCG               CG[GA][CT]^CG
              375  CGGCCG              
Bsp1407I      654  TGTACA               T^GTACA
             4894  TGTACA              
BspLU11I     2162  ACATGT               A^CATGT
             4891  ACATGT              
BsrGI         654  TGTACA               T^GTACA
             4894  TGTACA              
BssSI        1051  CACGAG               CACGAG(-5/-1)
             3542  CACGAG              
DraII         588  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2281  GGGTCCT             
EagI          101  CGGCCG               C^GGCCG
              375  CGGCCG              
EclXI         101  CGGCCG               C^GGCCG
              375  CGGCCG              
Eco52I        101  CGGCCG               C^GGCCG
              375  CGGCCG              
EcoO109I      588  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2281  GGGTCCT             
EcoRV        1013  GATATC               GAT^ATC
             2848  GATATC              
EcoT22I       933  ATGCAT               ATGCA^T
             5670  ATGCAT              
HgaI          399  GACGC                GACGC(5/10)
             4312  GACGC               
MluNI        3666  TGGCCA               TGG^CCA
             5749  TGGCCA              
MscI         3666  TGGCCA               TGG^CCA
             5749  TGGCCA              
NsiI          933  ATGCAT               ATGCA^T
             5670  ATGCAT              
PmaCI         578  CACGTG               CAC^GTG
             5372  CACGTG              
PmlI          578  CACGTG               CAC^GTG
             5372  CACGTG              
PpuMI         588  GGGACCT              [GA]G^G[AT]CC[CT]
             2281  GGGTCCT             
SacI         3423  GAGCTC               GAGCT^C
             3446  GAGCTC              
SmaI          340  CCCGGG               CCC^GGG
              363  CCCGGG              
SspBI         654  TGTACA               T^GTACA
             4894  TGTACA              
XbaI          793  TCTAGA               T^CTAGA
             3151  TCTAGA              
XmaCI         340  CCCGGG               C^CCGGG
              363  CCCGGG              
XmaI          340  CCCGGG               C^CCGGG
              363  CCCGGG              
XmnI         1068  GAAGAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2708  GAAACTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflII       AflIII      AluI        AlwI        AlwNI       
ApoI        AseI        AsnI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BmyI        BpuAI       BsaHI       BsaI        
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspHI       
BspMI       BsrBI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DsaI        EaeI        EarI        EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PshBI       PstI        PvuII       
RcaI        RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII