################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspI AviII BseAI BsiWI BsmBI BspEI BssHII BstBI BstEII BstPI CpoI DraIII Eam1105I Eco47III EcoNI EcoO65I EcoRI FseI FspI HpaI MfeI MluI MroI MunI NdeI NheI NotI NruI NspV PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Tth111I ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 2991 GACGTC GACGT^C Acc65I 5484 GGTACC G^GTACC AccI 5841 GTATAC GT^[AC][GT]AC AocI 2285 CCTGAGG CC^T[ACGT]AGG Asp718I 5484 GGTACC G^GTACC BamHI 825 GGATCC G^GATCC BbeI 265 GGCGCC GGCGC^C BcgI 3196 GCACACAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2212 GGATAC GTATCC(6/5) BlpI 3782 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3782 GCTCAGC GC^T[ACGT]AGC BsaBI 2844 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4113 ACCGGA [AT]^CCGG[AT] BspDI 2845 ATCGAT AT^CGAT BsrDI 1733 GCAATG GCAATG(2/0) BsrFI 99 GCCGGC [GA]^CCGG[CT] Bst1107I 5841 GTATAC GTA^TAC BstXI 1283 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 5841 GTATAC GTA^TAC Bsu36I 2285 CCTGAGG CC^T[ACGT]AGG CelII 3782 GCTCAGC GC^T[ACGT]AGC Cfr10I 99 GCCGGC [GA]^CCGG[CT] ClaI 2845 ATCGAT AT^CGAT DrdI 4034 GACTGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2285 CCTGAGG CC^T[ACGT]AGG HincII 4602 GTTGAC GT[CT]^[GA]AC HindII 4602 GTTGAC GT[CT]^[GA]AC KasI 265 GGCGCC G^GCGCC KpnI 5484 GGTACC GGTAC^C MamI 2844 GATCGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 99 GCCGGC GCC^GGC NarI 265 GGCGCC GG^CGCC NcoI 2274 CCATGG C^CATGG NgoMIV 99 GCCGGC G^CCGGC PacI 2261 TTAATTAA TTAAT^TAA PaeR7I 3426 CTCGAG C^TCGAG PflMI 3877 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 3029 GAAGAGC GCTCTTC(1/4) SbfI 5077 CCTGCAGG CCTGCA^GG SfoI 265 GGCGCC GGC^GCC Sse8387I 5077 CCTGCAGG CCTGCA^GG StuI 5074 AGGCCT AGG^CCT SwaI 5540 ATTTAAAT ATTT^AAAT Van91I 3877 CCACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3654 CCAAGACTCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3426 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### Asp700I 1068 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2708 GAAACTATTC AvrII 40 CCTAGG C^CTAGG 3966 CCTAGG BalI 3666 TGGCCA TGG^CCA 5749 TGGCCA BbrPI 578 CACGTG CAC^GTG 5372 CACGTG BbvCI 2624 CCTCAGC CCTCAGC(-5/-2) 2207 GCTGAGG BlnI 40 CCTAGG C^CTAGG 3966 CCTAGG BpmI 135 CTGGAG CTGGAG(16/14) 2028 CTGGAG BsaAI 578 CACGTG [CT]AC^GT[GA] 5372 CACGTG BseRI 32 GAGGAG GAGGAG(10/8) 1481 GAGGAG BsgI 3744 CTGCAC GTGCAG(16/14) 843 GTGCAG BsiEI 101 CGGCCG CG[GA][CT]^CG 375 CGGCCG Bsp1407I 654 TGTACA T^GTACA 4894 TGTACA BspLU11I 2162 ACATGT A^CATGT 4891 ACATGT BsrGI 654 TGTACA T^GTACA 4894 TGTACA BssSI 1051 CACGAG CACGAG(-5/-1) 3542 CACGAG DraII 588 GGGACCT [GA]G^G[ACGT]CC[CT] 2281 GGGTCCT EagI 101 CGGCCG C^GGCCG 375 CGGCCG EclXI 101 CGGCCG C^GGCCG 375 CGGCCG Eco52I 101 CGGCCG C^GGCCG 375 CGGCCG EcoO109I 588 GGGACCT [GA]G^G[ACGT]CC[CT] 2281 GGGTCCT EcoRV 1013 GATATC GAT^ATC 2848 GATATC EcoT22I 933 ATGCAT ATGCA^T 5670 ATGCAT HgaI 399 GACGC GACGC(5/10) 4312 GACGC MluNI 3666 TGGCCA TGG^CCA 5749 TGGCCA MscI 3666 TGGCCA TGG^CCA 5749 TGGCCA NsiI 933 ATGCAT ATGCA^T 5670 ATGCAT PmaCI 578 CACGTG CAC^GTG 5372 CACGTG PmlI 578 CACGTG CAC^GTG 5372 CACGTG PpuMI 588 GGGACCT [GA]G^G[AT]CC[CT] 2281 GGGTCCT SacI 3423 GAGCTC GAGCT^C 3446 GAGCTC SmaI 340 CCCGGG CCC^GGG 363 CCCGGG SspBI 654 TGTACA T^GTACA 4894 TGTACA XbaI 793 TCTAGA T^CTAGA 3151 TCTAGA XmaCI 340 CCCGGG C^CCGGG 363 CCCGGG XmaI 340 CCCGGG C^CCGGG 363 CCCGGG XmnI 1068 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2708 GAAACTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflII AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BanI BanII BbsI BbvI BclI BcnI BfaI BfrI BglI BglII BmyI BpuAI BsaHI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PshBI PstI PvuII RcaI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII