################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccIII AflII AgeI AscI AseI AsnI Asp718I AviII AvrII BamHI BbeI BbrPI BfrI BlnI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI BstAPI BstEII BstPI ClaI CpoI EcoO65I FauI FseI FspI HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI ScaI SfiI SfoI SgrAI SnaBI SpeI SphI SspBI SwaI XhoI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AclI 516 AACGTT AA^CGTT AcyI 3445 GGCGTC G[GA]^CG[CT]C AflIII 3956 ACACGT A^C[GA][CT]GT Alw44I 2398 GTGCAC G^TGCAC Aor51HI 1001 AGCGCT AGC^GCT ApaLI 2398 GTGCAC G^TGCAC AspI 1075 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 3185 TGGCCA TGG^CCA BcgI 126 CGAAGAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3330 GTATCC GTATCC(6/5) BclI 1449 TGATCA T^GATCA BglI 1180 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 468 AGATCT A^GATCT BlpI 1559 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1559 GCTCAGC GC^T[ACGT]AGC BsaAI 2788 TACGTG [CT]AC^GT[GA] BsaHI 3445 GGCGTC G[GA]^CG[CT]C BsaWI 857 ACCGGA [AT]^CCGG[AT] BsiEI 3614 CGGCCG CG[GA][CT]^CG BsmBI 122 GAGACG CGTCTC(1/5) BspMI 3716 ACCTGC ACCTGC(4/8) BsrFI 3612 ACCGGC [GA]^CCGG[CT] Bst1107I 2937 GTATAC GTA^TAC BstBI 3209 TTCGAA TT^CGAA BstXI 2744 CCATTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2937 GTATAC GTA^TAC CelII 1559 GCTCAGC GC^T[ACGT]AGC Cfr10I 3612 ACCGGC [GA]^CCGG[CT] DraIII 1823 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 381 GACGATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 3614 CGGCCG C^GGCCG EclXI 3614 CGGCCG C^GGCCG Eco47III 1001 AGCGCT AGC^GCT Eco52I 3614 CGGCCG C^GGCCG EcoRI 3806 GAATTC G^AATTC EcoRV 832 GATATC GAT^ATC EcoT22I 2953 ATGCAT ATGCA^T FbaI 1449 TGATCA T^GATCA HaeII 1001 AGCGCT [GA]GCGC^[CT] Hin1I 3445 GGCGTC G[GA]^CG[CT]C MfeI 3865 CAATTG C^AATTG MluNI 3185 TGGCCA TGG^CCA MscI 3185 TGGCCA TGG^CCA MunI 3865 CAATTG C^AATTG NcoI 3441 CCATGG C^CATGG NsiI 2953 ATGCAT ATGCA^T NspV 3209 TTCGAA TT^CGAA PflFI 1075 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 516 AACGTT AA^CGTT SbfI 4003 CCTGCAGG CCTGCA^GG SexAI 3400 ACCTGGT A^CC[AT]GGT SfuI 3209 TTCGAA TT^CGAA SmaI 660 CCCGGG CCC^GGG Sse8387I 4003 CCTGCAGG CCTGCA^GG SspI 4168 AATATT AAT^ATT Tth111I 1075 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2151 TCTAGA T^CTAGA XmaCI 660 CCCGGG C^CCGGG XmaI 660 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 2937 GTATAC GT^[AC][GT]AC 3915 GTAGAC AccII 2614 CGCG CG^CG 3625 CGCG AhdI 3397 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3611 GACCGGCCGTC AlwNI 639 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1906 CAGAAACTG AocI 3489 CCTGAGG CC^T[ACGT]AGG 3786 CCTCAGG ApaI 1126 GGGCCC GGGCC^C 2976 GGGCCC Asp700I 2698 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3795 GAAGCTCTTC AspEI 3397 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3611 GACCGGCCGTC AvaI 660 CCCGGG C^[CT]CG[GA]G 2840 CCCGAG BbvCI 2053 GCTGAGG CCTCAGC(-5/-2) 3856 GCTGAGG BsaI 1936 GAGACC GGTCTC(1/5) 3609 GAGACC BsoBI 660 CCCGGG C^[CT]CG[GA]G 2840 CCCGAG BspHI 3633 TCATGA T^CATGA 4054 TCATGA BsrDI 1768 CATTGC GCAATG(2/0) 2956 CATTGC BstUI 2614 CGCG CG^CG 3625 CGCG Bsu36I 3489 CCTGAGG CC^T[ACGT]AGG 3786 CCTCAGG DraI 1008 TTTAAA TTT^AAA 4076 TTTAAA DsaI 3419 CCACGG C^C[GA][CT]GG 3441 CCATGG EaeI 3185 TGGCCA [CT]^GGCC[GA] 3614 CGGCCG Eam1105I 3397 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3611 GACCGGCCGTC Eco81I 3489 CCTGAGG CC^T[ACGT]AGG 3786 CCTCAGG MvnI 2614 CGCG CG^CG 3625 CGCG NspI 920 ACATGC [GA]CATG^[CT] 2951 ACATGC PpuMI 291 GGGACCT [GA]G^G[AT]CC[CT] 3782 AGGACCT RcaI 3633 TCATGA T^CATGA 4054 TCATGA StuI 760 AGGCCT AGG^CCT 2057 AGGCCT XcmI 1130 CCACCCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2231 CCAGATTTCTTGTGG XmnI 2698 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3795 GAAGCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII