##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AgeI        
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BamHI       BbeI        BbrPI       BfrI        
BlnI        BsaBI       BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrGI       
BssHII      BssSI       BstAPI      BstEII      BstPI       
ClaI        CpoI        EcoO65I     FauI        FseI        
FspI        HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       PvuI        RsrII       
SacII       SalI        ScaI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AclI          516  AACGTT               AA^CGTT
AcyI         3445  GGCGTC               G[GA]^CG[CT]C
AflIII       3956  ACACGT               A^C[GA][CT]GT
Alw44I       2398  GTGCAC               G^TGCAC
Aor51HI      1001  AGCGCT               AGC^GCT
ApaLI        2398  GTGCAC               G^TGCAC
AspI         1075  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         3185  TGGCCA               TGG^CCA
BcgI          126  CGAAGAAGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3330  GTATCC               GTATCC(6/5)
BclI         1449  TGATCA               T^GATCA
BglI         1180  GCCAAGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         468  AGATCT               A^GATCT
BlpI         1559  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1559  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2788  TACGTG               [CT]AC^GT[GA]
BsaHI        3445  GGCGTC               G[GA]^CG[CT]C
BsaWI         857  ACCGGA               [AT]^CCGG[AT]
BsiEI        3614  CGGCCG               CG[GA][CT]^CG
BsmBI         122  GAGACG               CGTCTC(1/5)
BspMI        3716  ACCTGC               ACCTGC(4/8)
BsrFI        3612  ACCGGC               [GA]^CCGG[CT]
Bst1107I     2937  GTATAC               GTA^TAC
BstBI        3209  TTCGAA               TT^CGAA
BstXI        2744  CCATTATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2937  GTATAC               GTA^TAC
CelII        1559  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       3612  ACCGGC               [GA]^CCGG[CT]
DraIII       1823  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          381  GACGATTGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         3614  CGGCCG               C^GGCCG
EclXI        3614  CGGCCG               C^GGCCG
Eco47III     1001  AGCGCT               AGC^GCT
Eco52I       3614  CGGCCG               C^GGCCG
EcoRI        3806  GAATTC               G^AATTC
EcoRV         832  GATATC               GAT^ATC
EcoT22I      2953  ATGCAT               ATGCA^T
FbaI         1449  TGATCA               T^GATCA
HaeII        1001  AGCGCT               [GA]GCGC^[CT]
Hin1I        3445  GGCGTC               G[GA]^CG[CT]C
MfeI         3865  CAATTG               C^AATTG
MluNI        3185  TGGCCA               TGG^CCA
MscI         3185  TGGCCA               TGG^CCA
MunI         3865  CAATTG               C^AATTG
NcoI         3441  CCATGG               C^CATGG
NsiI         2953  ATGCAT               ATGCA^T
NspV         3209  TTCGAA               TT^CGAA
PflFI        1075  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I      516  AACGTT               AA^CGTT
SbfI         4003  CCTGCAGG             CCTGCA^GG
SexAI        3400  ACCTGGT              A^CC[AT]GGT
SfuI         3209  TTCGAA               TT^CGAA
SmaI          660  CCCGGG               CCC^GGG
Sse8387I     4003  CCTGCAGG             CCTGCA^GG
SspI         4168  AATATT               AAT^ATT
Tth111I      1075  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2151  TCTAGA               T^CTAGA
XmaCI         660  CCCGGG               C^CCGGG
XmaI          660  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI         2937  GTATAC               GT^[AC][GT]AC
             3915  GTAGAC              
AccII        2614  CGCG                 CG^CG
             3625  CGCG                
AhdI         3397  GACACCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3611  GACCGGCCGTC         
AlwNI         639  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1906  CAGAAACTG           
AocI         3489  CCTGAGG              CC^T[ACGT]AGG
             3786  CCTCAGG             
ApaI         1126  GGGCCC               GGGCC^C
             2976  GGGCCC              
Asp700I      2698  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3795  GAAGCTCTTC          
AspEI        3397  GACACCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3611  GACCGGCCGTC         
AvaI          660  CCCGGG               C^[CT]CG[GA]G
             2840  CCCGAG              
BbvCI        2053  GCTGAGG              CCTCAGC(-5/-2)
             3856  GCTGAGG             
BsaI         1936  GAGACC               GGTCTC(1/5)
             3609  GAGACC              
BsoBI         660  CCCGGG               C^[CT]CG[GA]G
             2840  CCCGAG              
BspHI        3633  TCATGA               T^CATGA
             4054  TCATGA              
BsrDI        1768  CATTGC               GCAATG(2/0)
             2956  CATTGC              
BstUI        2614  CGCG                 CG^CG
             3625  CGCG                
Bsu36I       3489  CCTGAGG              CC^T[ACGT]AGG
             3786  CCTCAGG             
DraI         1008  TTTAAA               TTT^AAA
             4076  TTTAAA              
DsaI         3419  CCACGG               C^C[GA][CT]GG
             3441  CCATGG              
EaeI         3185  TGGCCA               [CT]^GGCC[GA]
             3614  CGGCCG              
Eam1105I     3397  GACACCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3611  GACCGGCCGTC         
Eco81I       3489  CCTGAGG              CC^T[ACGT]AGG
             3786  CCTCAGG             
MvnI         2614  CGCG                 CG^CG
             3625  CGCG                
NspI          920  ACATGC               [GA]CATG^[CT]
             2951  ACATGC              
PpuMI         291  GGGACCT              [GA]G^G[AT]CC[CT]
             3782  AGGACCT             
RcaI         3633  TCATGA               T^CATGA
             4054  TCATGA              
StuI          760  AGGCCT               AGG^CCT
             2057  AGGCCT              
XcmI         1130  CCACCCATGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2231  CCAGATTTCTTGTGG     
XmnI         2698  GAAAATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3795  GAAGCTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII