##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I ApaLI AscI
AspEI BbeI BbvCI BcgI BclI
BfrI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspEI BsrGI BssHII BstBI CelII
CpoI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FbaI FseI Hin1I
KasI MluI MroI NarI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 4087 GGTACC G^GTACC
Aor51HI 258 AGCGCT AGC^GCT
ApaI 3207 GGGCCC GGGCC^C
AseI 4011 ATTAAT AT^TAAT
AsnI 4011 ATTAAT AT^TAAT
Asp718I 4087 GGTACC G^GTACC
AviII 613 TGCGCA TGC^GCA
BspDI 608 ATCGAT AT^CGAT
BspLU11I 1544 ACATGT A^CATGT
BsrDI 2453 CATTGC GCAATG(2/0)
Bst1107I 1947 GTATAC GTA^TAC
BstAPI 1468 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3442 GGTGACC G^GT[ACGT]ACC
BstPI 3442 GGTGACC G^GT[ACGT]ACC
BstZ17I 1947 GTATAC GTA^TAC
ClaI 608 ATCGAT AT^CGAT
Eco47III 258 AGCGCT AGC^GCT
EcoO65I 3442 GGTGACC G^GT[ACGT]ACC
EcoRI 3479 GAATTC G^AATTC
FspI 613 TGCGCA TGC^GCA
HpaI 4221 GTTAAC GTT^AAC
KpnI 4087 GGTACC GGTAC^C
MfeI 3259 CAATTG C^AATTG
MunI 3259 CAATTG C^AATTG
NcoI 237 CCATGG C^CATGG
NdeI 4130 CATATG CA^TATG
PpuMI 3672 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 4011 ATTAAT AT^TAAT
SapI 661 GCTCTTC GCTCTTC(1/4)
SfiI 1708 GGCCTGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 473 GCATGC GCATG^C
SspI 2034 AATATT AAT^ATT
XbaI 736 TCTAGA T^CTAGA
XcmI 3312 CCAGGGCAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1947 GTATAC GT^[AC][GT]AC
2329 GTAGAC
AlwNI 1493 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1796 CAGTGGCTG
Asp700I 1228 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3149 GAAAGACTTC
AvaI 152 CCCGGG C^[CT]CG[GA]G
1704 CCCGGG
AvrII 1924 CCTAGG C^CTAGG
3501 CCTAGG
BalI 121 TGGCCA TGG^CCA
3526 TGGCCA
BamHI 131 GGATCC G^GATCC
684 GGATCC
BanI 1919 GGTGCC G^G[CT][GA]CC
4087 GGTACC
BbrPI 1914 CACGTG CAC^GTG
4247 CACGTG
BciVI 3462 GGATAC GTATCC(6/5)
4180 GGATAC
BglII 739 AGATCT A^GATCT
876 AGATCT
BlnI 1924 CCTAGG C^CTAGG
3501 CCTAGG
BsaBI 838 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1072 GATGTTGATC
BsoBI 152 CCCGGG C^[CT]CG[GA]G
1704 CCCGGG
BspHI 927 TCATGA T^CATGA
2874 TCATGA
BssSI 21 CTCGTG CACGAG(-5/-1)
3583 CTCGTG
BstXI 246 CCACCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3578 CCATACTCGTGG
DraI 2019 TTTAAA TTT^AAA
2113 TTTAAA
HindIII 3133 AAGCTT A^AGCTT
3221 AAGCTT
KspI 52 CCGCGG CCGC^GG
76 CCGCGG
MamI 838 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1072 GATGTTGATC
MluNI 121 TGGCCA TGG^CCA
3526 TGGCCA
MscI 121 TGGCCA TGG^CCA
3526 TGGCCA
NaeI 174 GCCGGC GCC^GGC
1119 GCCGGC
NgoMIV 174 GCCGGC G^CCGGC
1119 GCCGGC
PmaCI 1914 CACGTG CAC^GTG
4247 CACGTG
PmlI 1914 CACGTG CAC^GTG
4247 CACGTG
RcaI 927 TCATGA T^CATGA
2874 TCATGA
SacII 52 CCGCGG CCGC^GG
76 CCGCGG
ScaI 2418 AGTACT AGT^ACT
2585 AGTACT
SmaI 152 CCCGGG CCC^GGG
1704 CCCGGG
XmaCI 152 CCCGGG C^CCGGG
1704 CCCGGG
XmaI 152 CCCGGG C^CCGGG
1704 CCCGGG
XmnI 1228 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3149 GAAAGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AocI ApoI AspHI
AspI AvaII BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII