##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AgeI        AhdI        Alw44I      ApaLI       AscI        
AspEI       BbeI        BbvCI       BcgI        BclI        
BfrI        BlpI        Bpu1102I    BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspEI       BsrGI       BssHII      BstBI       CelII       
CpoI        EagI        Eam1105I    EclXI       Eco52I      
EcoRV       EcoT22I     FbaI        FseI        Hin1I       
KasI        MluI        MroI        NarI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       Psp1406I    
PvuI        RsrII       SalI        SbfI        SexAI       
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I       4087  GGTACC               G^GTACC
Aor51HI       258  AGCGCT               AGC^GCT
ApaI         3207  GGGCCC               GGGCC^C
AseI         4011  ATTAAT               AT^TAAT
AsnI         4011  ATTAAT               AT^TAAT
Asp718I      4087  GGTACC               G^GTACC
AviII         613  TGCGCA               TGC^GCA
BspDI         608  ATCGAT               AT^CGAT
BspLU11I     1544  ACATGT               A^CATGT
BsrDI        2453  CATTGC               GCAATG(2/0)
Bst1107I     1947  GTATAC               GTA^TAC
BstAPI       1468  GCAGGCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3442  GGTGACC              G^GT[ACGT]ACC
BstPI        3442  GGTGACC              G^GT[ACGT]ACC
BstZ17I      1947  GTATAC               GTA^TAC
ClaI          608  ATCGAT               AT^CGAT
Eco47III      258  AGCGCT               AGC^GCT
EcoO65I      3442  GGTGACC              G^GT[ACGT]ACC
EcoRI        3479  GAATTC               G^AATTC
FspI          613  TGCGCA               TGC^GCA
HpaI         4221  GTTAAC               GTT^AAC
KpnI         4087  GGTACC               GGTAC^C
MfeI         3259  CAATTG               C^AATTG
MunI         3259  CAATTG               C^AATTG
NcoI          237  CCATGG               C^CATGG
NdeI         4130  CATATG               CA^TATG
PpuMI        3672  AGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        4011  ATTAAT               AT^TAAT
SapI          661  GCTCTTC              GCTCTTC(1/4)
SfiI         1708  GGCCTGGCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          473  GCATGC               GCATG^C
SspI         2034  AATATT               AAT^ATT
XbaI          736  TCTAGA               T^CTAGA
XcmI         3312  CCAGGGCAAATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI         1947  GTATAC               GT^[AC][GT]AC
             2329  GTAGAC              
AlwNI        1493  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1796  CAGTGGCTG           
Asp700I      1228  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3149  GAAAGACTTC          
AvaI          152  CCCGGG               C^[CT]CG[GA]G
             1704  CCCGGG              
AvrII        1924  CCTAGG               C^CTAGG
             3501  CCTAGG              
BalI          121  TGGCCA               TGG^CCA
             3526  TGGCCA              
BamHI         131  GGATCC               G^GATCC
              684  GGATCC              
BanI         1919  GGTGCC               G^G[CT][GA]CC
             4087  GGTACC              
BbrPI        1914  CACGTG               CAC^GTG
             4247  CACGTG              
BciVI        3462  GGATAC               GTATCC(6/5)
             4180  GGATAC              
BglII         739  AGATCT               A^GATCT
              876  AGATCT              
BlnI         1924  CCTAGG               C^CTAGG
             3501  CCTAGG              
BsaBI         838  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1072  GATGTTGATC          
BsoBI         152  CCCGGG               C^[CT]CG[GA]G
             1704  CCCGGG              
BspHI         927  TCATGA               T^CATGA
             2874  TCATGA              
BssSI          21  CTCGTG               CACGAG(-5/-1)
             3583  CTCGTG              
BstXI         246  CCACCGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3578  CCATACTCGTGG        
DraI         2019  TTTAAA               TTT^AAA
             2113  TTTAAA              
HindIII      3133  AAGCTT               A^AGCTT
             3221  AAGCTT              
KspI           52  CCGCGG               CCGC^GG
               76  CCGCGG              
MamI          838  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1072  GATGTTGATC          
MluNI         121  TGGCCA               TGG^CCA
             3526  TGGCCA              
MscI          121  TGGCCA               TGG^CCA
             3526  TGGCCA              
NaeI          174  GCCGGC               GCC^GGC
             1119  GCCGGC              
NgoMIV        174  GCCGGC               G^CCGGC
             1119  GCCGGC              
PmaCI        1914  CACGTG               CAC^GTG
             4247  CACGTG              
PmlI         1914  CACGTG               CAC^GTG
             4247  CACGTG              
RcaI          927  TCATGA               T^CATGA
             2874  TCATGA              
SacII          52  CCGCGG               CCGC^GG
               76  CCGCGG              
ScaI         2418  AGTACT               AGT^ACT
             2585  AGTACT              
SmaI          152  CCCGGG               CCC^GGG
             1704  CCCGGG              
XmaCI         152  CCCGGG               C^CCGGG
             1704  CCCGGG              
XmaI          152  CCCGGG               C^CCGGG
             1704  CCCGGG              
XmnI         1228  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3149  GAAAGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AocI        ApoI        AspHI       
AspI        AvaII       BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DrdI        DsaI        EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PflMI       PleI        PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XhoII