##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
AocI        Aor51HI     AscI        Asp718I     AspEI       
AviII       BbeI        BbrPI       BbvCI       BglI        
BglII       BlpI        Bpu1102I    BsaBI       BseAI       
BsiEI       BsiWI       BspDI       BspEI       BspHI       
BspMI       BssHII      BstAPI      BstBI       BstEII      
BstPI       Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     FseI        
FspI        HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        NarI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        3037  GACGTC               GACGT^C
AccI          434  GTATAC               GT^[AC][GT]AC
AflII        3710  CTTAAG               C^TTAAG
Alw44I       3982  GTGCAC               G^TGCAC
AlwNI         951  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        3982  GTGCAC               G^TGCAC
AseI         1764  ATTAAT               AT^TAAT
AsnI         1764  ATTAAT               AT^TAAT
AspI          126  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        3549  GGATCC               G^GATCC
BcgI           57  CGAGACACTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          464  TGATCA               T^GATCA
BfrI         3710  CTTAAG               C^TTAAG
BsaAI         286  CACGTA               [CT]AC^GT[GA]
BsaI          313  GAGACC               GGTCTC(1/5)
BsmBI        2676  CGTCTC               CGTCTC(1/5)
Bsp1407I      365  TGTACA               T^GTACA
BspLU11I     2318  ACATGT               A^CATGT
BsrGI         365  TGTACA               T^GTACA
Bst1107I      434  GTATAC               GTA^TAC
BstZ17I       434  GTATAC               GTA^TAC
DsaI         1702  CCATGG               C^C[GA][CT]GG
EcoRI        1620  GAATTC               G^AATTC
EcoRV        4058  GATATC               GAT^ATC
FbaI          464  TGATCA               T^GATCA
HincII       1677  GTTGAC               GT[CT]^[GA]AC
HindII       1677  GTTGAC               GT[CT]^[GA]AC
MfeI         3694  CAATTG               C^AATTG
MunI         3694  CAATTG               C^AATTG
NaeI         3294  GCCGGC               GCC^GGC
NcoI         1702  CCATGG               C^CATGG
NgoMIV       3294  GCCGGC               G^CCGGC
NspI         2318  ACATGT               [GA]CATG^[CT]
PflFI         126  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1829  GGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        3319  GACAGCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1764  ATTAAT               AT^TAAT
SacI          628  GAGCTC               GAGCT^C
SspBI         365  TGTACA               T^GTACA
StuI         3562  AGGCCT               AGG^CCT
Tth111I       126  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          806  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII          91  CGCG                 CG^CG
             1659  CGCG                
AcyI         1511  GGCGTC               G[GA]^CG[CT]C
             3037  GACGTC              
AflIII       1912  ACGTGT               A^C[GA][CT]GT
             2318  ACATGT              
ApaI         2260  GGGCCC               GGGCC^C
             2719  GGGCCC              
AvrII        1484  CCTAGG               C^CTAGG
             1694  CCTAGG              
BalI         1984  TGGCCA               TGG^CCA
             3515  TGGCCA              
BciVI         344  GGATAC               GTATCC(6/5)
             1307  GTATCC              
BlnI         1484  CCTAGG               C^CTAGG
             1694  CCTAGG              
BsaHI        1511  GGCGTC               G[GA]^CG[CT]C
             3037  GACGTC              
BsaWI         158  ACCGGA               [AT]^CCGG[AT]
             3375  TCCGGT              
BsrBI          46  GAGCGG               CCGCTC(-3/-3)
             2598  GAGCGG              
BsrDI         652  CATTGC               GCAATG(2/0)
              702  CATTGC              
BsrFI          36  ACCGGC               [GA]^CCGG[CT]
             3294  GCCGGC              
BssSI        2083  CTCGTG               CACGAG(-5/-1)
             3795  CTCGTG              
BstUI          91  CGCG                 CG^CG
             1659  CGCG                
Cfr10I         36  ACCGGC               [GA]^CCGG[CT]
             3294  GCCGGC              
DraIII       1876  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2326  CACCCAGTG           
EcoNI         500  CCTACTTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1484  CCTAGGGGAGG         
EcoT22I       967  ATGCAT               ATGCA^T
             1162  ATGCAT              
HaeII         227  GGCGCT               [GA]GCGC^[CT]
             2181  AGCGCC              
HgaI         1512  GCGTC                GACGC(5/10)
             1964  GCGTC               
Hin1I        1511  GGCGTC               G[GA]^CG[CT]C
             3037  GACGTC              
MluNI        1984  TGGCCA               TGG^CCA
             3515  TGGCCA              
MscI         1984  TGGCCA               TGG^CCA
             3515  TGGCCA              
MvnI           91  CGCG                 CG^CG
             1659  CGCG                
NdeI          212  CATATG               CA^TATG
             3838  CATATG              
NheI         1019  GCTAGC               G^CTAGC
             1898  GCTAGC              
NsiI          967  ATGCAT               ATGCA^T
             1162  ATGCAT              
PstI         3850  CTGCAG               CTGCA^G
             4119  CTGCAG              
SmaI         2257  CCCGGG               CCC^GGG
             2572  CCCGGG              
SmlI          504  CTTGAG               C^T[CT][GA]AG
             3710  CTTAAG              
XcmI         1466  CCATTTTAGACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3272  CCAGAACAAGTCTGG     
XmaCI        2257  CCCGGG               C^CCGGG
             2572  CCCGGG              
XmaI         2257  CCCGGG               C^CCGGG
             2572  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaI        AvaII       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        RsaI        
SapI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI