################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AccIII AclI AgeI AhdI AocI Aor51HI AscI Asp718I AspEI AviII BbeI BbrPI BbvCI BglI BglII BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BspDI BspEI BspHI BspMI BssHII BstAPI BstBI BstEII BstPI Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I FseI FspI HpaI KasI KpnI KspI MamI MluI MroI NarI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 3037 GACGTC GACGT^C AccI 434 GTATAC GT^[AC][GT]AC AflII 3710 CTTAAG C^TTAAG Alw44I 3982 GTGCAC G^TGCAC AlwNI 951 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 3982 GTGCAC G^TGCAC AseI 1764 ATTAAT AT^TAAT AsnI 1764 ATTAAT AT^TAAT AspI 126 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 3549 GGATCC G^GATCC BcgI 57 CGAGACACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 464 TGATCA T^GATCA BfrI 3710 CTTAAG C^TTAAG BsaAI 286 CACGTA [CT]AC^GT[GA] BsaI 313 GAGACC GGTCTC(1/5) BsmBI 2676 CGTCTC CGTCTC(1/5) Bsp1407I 365 TGTACA T^GTACA BspLU11I 2318 ACATGT A^CATGT BsrGI 365 TGTACA T^GTACA Bst1107I 434 GTATAC GTA^TAC BstZ17I 434 GTATAC GTA^TAC DsaI 1702 CCATGG C^C[GA][CT]GG EcoRI 1620 GAATTC G^AATTC EcoRV 4058 GATATC GAT^ATC FbaI 464 TGATCA T^GATCA HincII 1677 GTTGAC GT[CT]^[GA]AC HindII 1677 GTTGAC GT[CT]^[GA]AC MfeI 3694 CAATTG C^AATTG MunI 3694 CAATTG C^AATTG NaeI 3294 GCCGGC GCC^GGC NcoI 1702 CCATGG C^CATGG NgoMIV 3294 GCCGGC G^CCGGC NspI 2318 ACATGT [GA]CATG^[CT] PflFI 126 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1829 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 3319 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1764 ATTAAT AT^TAAT SacI 628 GAGCTC GAGCT^C SspBI 365 TGTACA T^GTACA StuI 3562 AGGCCT AGG^CCT Tth111I 126 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 806 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccII 91 CGCG CG^CG 1659 CGCG AcyI 1511 GGCGTC G[GA]^CG[CT]C 3037 GACGTC AflIII 1912 ACGTGT A^C[GA][CT]GT 2318 ACATGT ApaI 2260 GGGCCC GGGCC^C 2719 GGGCCC AvrII 1484 CCTAGG C^CTAGG 1694 CCTAGG BalI 1984 TGGCCA TGG^CCA 3515 TGGCCA BciVI 344 GGATAC GTATCC(6/5) 1307 GTATCC BlnI 1484 CCTAGG C^CTAGG 1694 CCTAGG BsaHI 1511 GGCGTC G[GA]^CG[CT]C 3037 GACGTC BsaWI 158 ACCGGA [AT]^CCGG[AT] 3375 TCCGGT BsrBI 46 GAGCGG CCGCTC(-3/-3) 2598 GAGCGG BsrDI 652 CATTGC GCAATG(2/0) 702 CATTGC BsrFI 36 ACCGGC [GA]^CCGG[CT] 3294 GCCGGC BssSI 2083 CTCGTG CACGAG(-5/-1) 3795 CTCGTG BstUI 91 CGCG CG^CG 1659 CGCG Cfr10I 36 ACCGGC [GA]^CCGG[CT] 3294 GCCGGC DraIII 1876 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG 2326 CACCCAGTG EcoNI 500 CCTACTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1484 CCTAGGGGAGG EcoT22I 967 ATGCAT ATGCA^T 1162 ATGCAT HaeII 227 GGCGCT [GA]GCGC^[CT] 2181 AGCGCC HgaI 1512 GCGTC GACGC(5/10) 1964 GCGTC Hin1I 1511 GGCGTC G[GA]^CG[CT]C 3037 GACGTC MluNI 1984 TGGCCA TGG^CCA 3515 TGGCCA MscI 1984 TGGCCA TGG^CCA 3515 TGGCCA MvnI 91 CGCG CG^CG 1659 CGCG NdeI 212 CATATG CA^TATG 3838 CATATG NheI 1019 GCTAGC G^CTAGC 1898 GCTAGC NsiI 967 ATGCAT ATGCA^T 1162 ATGCAT PstI 3850 CTGCAG CTGCA^G 4119 CTGCAG SmaI 2257 CCCGGG CCC^GGG 2572 CCCGGG SmlI 504 CTTGAG C^T[CT][GA]AG 3710 CTTAAG XcmI 1466 CCATTTTAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3272 CCAGAACAAGTCTGG XmaCI 2257 CCCGGG C^CCGGG 2572 CCCGGG XmaI 2257 CCCGGG C^CCGGG 2572 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI