##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I AocI ApaLI AscI Asp718I
AspHI AspI AviII AvrII BalI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiHKAI
BsiWI BspEI BspHI BsrDI BssHII
BssSI BstBI BstXI Bsu36I CelII
CpoI DraIII DrdI DsaI Eco81I
EcoNI EcoRV FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NciI
NcoI NotI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I Tth111I
Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcyI 80 GGCGCC G[GA]^CG[CT]C
AflII 1845 CTTAAG C^TTAAG
AlwNI 558 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1132 AGCGCT AGC^GCT
ApaI 49 GGGCCC GGGCC^C
Asp700I 348 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 761 CTCGAG C^[CT]CG[GA]G
BamHI 2995 GGATCC G^GATCC
BbeI 80 GGCGCC GGCGC^C
BfrI 1845 CTTAAG C^TTAAG
BglI 83 GCCATTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 80 GGCGCC G[GA]^CG[CT]C
BsaI 2494 GAGACC GGTCTC(1/5)
BsiEI 90 CGGCCG CG[GA][CT]^CG
BsmBI 1394 GAGACG CGTCTC(1/5)
BsoBI 761 CTCGAG C^[CT]CG[GA]G
BspDI 236 ATCGAT AT^CGAT
BspMI 143 GCAGGT ACCTGC(4/8)
BsrBI 115 GAGCGG CCGCTC(-3/-3)
BsrFI 6 GCCGGC [GA]^CCGG[CT]
Bst1107I 2254 GTATAC GTA^TAC
BstAPI 1705 GCAACCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2156 GGTTACC G^GT[ACGT]ACC
BstPI 2156 GGTTACC G^GT[ACGT]ACC
BstYI 2995 GGATCC [GA]^GATC[CT]
BstZ17I 2254 GTATAC GTA^TAC
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
ClaI 236 ATCGAT AT^CGAT
EagI 90 CGGCCG C^GGCCG
EclXI 90 CGGCCG C^GGCCG
Eco47III 1132 AGCGCT AGC^GCT
Eco52I 90 CGGCCG C^GGCCG
EcoO65I 2156 GGTTACC G^GT[ACGT]ACC
EcoT14I 330 CCTTGG C^C[AT][AT]GG
EcoT22I 398 ATGCAT ATGCA^T
HgaI 470 GCGTC GACGC(5/10)
Hin1I 80 GGCGCC G[GA]^CG[CT]C
KasI 80 GGCGCC G^GCGCC
MflI 2995 GGATCC [GA]^GATC[CT]
NaeI 6 GCCGGC GCC^GGC
NarI 80 GGCGCC GG^CGCC
NdeI 1780 CATATG CA^TATG
NgoMIV 6 GCCGGC G^CCGGC
NheI 864 GCTAGC G^CTAGC
NruI 99 TCGCGA TCG^CGA
NsiI 398 ATGCAT ATGCA^T
PaeR7I 761 CTCGAG C^TCGAG
PstI 357 CTGCAG CTGCA^G
SfoI 80 GGCGCC GGC^GCC
SspI 2766 AATATT AAT^ATT
StuI 2269 AGGCCT AGG^CCT
StyI 330 CCTTGG C^C[AT][AT]GG
SwaI 3238 ATTTAAAT ATTT^AAAT
XcmI 2115 CCAGTATTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 761 CTCGAG C^TCGAG
XhoII 2995 GGATCC [GA]^GATC[CT]
XmnI 348 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 100 CGCG CG^CG
121 CGCG
AhdI 1883 GACTCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2339 GACGTTTTGTC
AspEI 1883 GACTCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2339 GACGTTTTGTC
BanI 80 GGCGCC G^G[CT][GA]CC
3142 GGCACC
BanII 49 GGGCCC G[GA]GC[CT]^C
2074 GGGCTC
BmyI 49 GGGCCC G[AGT]GC[ACT]^C
2074 GGGCTC
BsgI 1016 CTGCAC GTGCAG(16/14)
797 GTGCAG
BsiYI 7 CCGGCTTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2010 CCCTAAATTGG
BslI 7 CCGGCTTCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2010 CCCTAAATTGG
BsmFI 15 GGGAC GGGAC(10/14)
1573 GGGAC
BsmI 716 GAATGC GAATGC(1/-1)
3416 GAATGC
Bsp1286I 49 GGGCCC G[AGT]GC[ACT]^C
2074 GGGCTC
Bsp1407I 1179 TGTACA T^GTACA
3405 TGTACA
BspLU11I 2461 ACATGT A^CATGT
2950 ACATGT
BsrGI 1179 TGTACA T^GTACA
3405 TGTACA
BstF5I 297 CATCC GGATG(2/0)
1172 CATCC
BstUI 100 CGCG CG^CG
121 CGCG
EaeI 90 CGGCCG [CT]^GGCC[GA]
105 CGGCCA
Eam1105I 1883 GACTCGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2339 GACGTTTTGTC
FokI 297 CATCC GGATG(9/13)
1172 CATCC
HapII 7 CCGG C^CGG
89 CCGG
HpaII 7 CCGG C^CGG
89 CCGG
MslI 1911 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2194 CACACTGATG
MspI 7 CCGG C^CGG
89 CCGG
MvnI 100 CGCG CG^CG
121 CGCG
PvuII 1953 CAGCTG CAG^CTG
2296 CAGCTG
ScaI 3055 AGTACT AGT^ACT
3550 AGTACT
SspBI 1179 TGTACA T^GTACA
3405 TGTACA
TfiI 898 GAATC G^A[AT]TC
2444 GATTC
XbaI 993 TCTAGA T^CTAGA
2555 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AvaII BbsI BbvI BfaI BpmI
BpuAI BsaJI BseRI BsmAI BsrI
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRI EcoRII Fnu4HI HaeII
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I