##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AgeI        
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        AspEI       AviII       AvrII       BalI        
BbeI        BbvCI       BcgI        BclI        BglI        
BlnI        BsaHI       BsaI        BsaWI       BseAI       
BsiWI       BsmBI       BsmFI       BsmI        BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BssHII      
BssSI       BstAPI      BstBI       Cfr10I      ClaI        
CpoI        DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoRI       EcoRV       FbaI        FseI        FspI        
HaeII       Hin1I       KasI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NcoI        NgoMIV      NotI        
NruI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SwaI        
Van91I      XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   69 enzymes) #
###################################

Acc65I        641  GGTACC               G^GTACC
AccI         1640  GTATAC               GT^[AC][GT]AC
AccII         101  CGCG                 CG^CG
AflII         736  CTTAAG               C^TTAAG
AflIII       1337  ACGTGT               A^C[GA][CT]GT
AlwNI        1466  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2412  CCTAAGG              CC^T[ACGT]AGG
Asp718I       641  GGTACC               G^GTACC
AspI         3324  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1327  CTCGGG               C^[CT]CG[GA]G
BamHI         979  GGATCC               G^GATCC
BbrPI        1336  CACGTG               CAC^GTG
BbsI         1581  GTCTTC               GAAGAC(2/6)
BciVI        3383  GTATCC               GTATCC(6/5)
BcnI         2447  CCGGG                CC^[GC]GG
BfrI          736  CTTAAG               C^TTAAG
BlpI         1237  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1237  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1581  GTCTTC               GAAGAC(2/6)
BsaAI        1336  CACGTG               [CT]AC^GT[GA]
BsaBI        1709  GATTTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         103  CGACCG               CG[GA][CT]^CG
BsoBI        1327  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     1861  TGTACA               T^GTACA
BsrGI        1861  TGTACA               T^GTACA
Bst1107I     1640  GTATAC               GTA^TAC
BstEII       1125  GGTGACC              G^GT[ACGT]ACC
BstPI        1125  GGTGACC              G^GT[ACGT]ACC
BstUI         101  CGCG                 CG^CG
BstXI          19  CCATCTTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1640  GTATAC               GTA^TAC
Bsu36I       2412  CCTAAGG              CC^T[ACGT]AGG
CelII        1237  GCTGAGC              GC^T[ACGT]AGC
CfoI         1279  GCGC                 GCG^C
EarI          523  GAAGAG               CTCTTC(1/4)
Eco81I       2412  CCTAAGG              CC^T[ACGT]AGG
EcoO65I      1125  GGTGACC              G^GT[ACGT]ACC
EcoT22I      1410  ATGCAT               ATGCA^T
HapII        2447  CCGG                 C^CGG
HgaI          577  GACGC                GACGC(5/10)
HhaI         1279  GCGC                 GCG^C
HinP1I       1279  GCGC                 G^CGC
HincII       1930  GTTAAC               GT[CT]^[GA]AC
HindII       1930  GTTAAC               GT[CT]^[GA]AC
HindIII       245  AAGCTT               A^AGCTT
HpaI         1930  GTTAAC               GTT^AAC
HpaII        2447  CCGG                 C^CGG
KpnI          641  GGTACC               GGTAC^C
Ksp632I       523  GAAGAG               CTCTTC(1/4)
MamI         1709  GATTTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI         2447  CCGG                 C^CGG
MvnI          101  CGCG                 CG^CG
NciI         2447  CCGGG                CC^[GC]GG
NdeI         3171  CATATG               CA^TATG
NheI          955  GCTAGC               G^CTAGC
NsiI         1410  ATGCAT               ATGCA^T
PflFI        3324  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1336  CACGTG               CAC^GTG
PmlI         1336  CACGTG               CAC^GTG
PstI          588  CTGCAG               CTGCA^G
PvuII        1420  CAGCTG               CAG^CTG
SapI          523  GAAGAGC              GCTCTTC(1/4)
SexAI        2674  ACCTGGT              A^CC[AT]GGT
SphI         1250  GCATGC               GCATG^C
SspBI        1861  TGTACA               T^GTACA
SspI          784  AATATT               AAT^ATT
StuI         2416  AGGCCT               AGG^CCT
Tth111I      3324  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1717  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

Alw44I       1351  GTGCAC               G^TGCAC
             2690  GTGCAC              
ApaLI        1351  GTGCAC               G^TGCAC
             2690  GTGCAC              
Asp700I      1397  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1577  GAATGTCTTC          
BsgI         2293  CTGCAC               GTGCAG(16/14)
             2536  GTGCAG              
BsrBI         657  GAGCGG               CCGCTC(-3/-3)
              835  GAGCGG              
BsrDI         798  CATTGC               GCAATG(2/0)
             1544  CATTGC              
DraII        1133  AGGACCT              [GA]G^G[ACGT]CC[CT]
             3231  AGGACCC             
DraIII       1333  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1557  CACTCTGTG           
EcoO109I     1133  AGGACCT              [GA]G^G[ACGT]CC[CT]
             3231  AGGACCC             
EcoT14I      1438  CCTTGG               C^C[AT][AT]GG
             2419  CCTTGG              
MaeII        1337  ACGT                 A^CGT
             1532  ACGT                
MspA1I       1420  CAGCTG               C[AC]G^C[GT]G
             2744  CAGCGG              
PpuMI        1133  AGGACCT              [GA]G^G[AT]CC[CT]
             3231  AGGACCC             
SacI          231  GAGCTC               GAGCT^C
             1240  GAGCTC              
ScaI         1522  AGTACT               AGT^ACT
             3185  AGTACT              
StyI         1438  CCTTGG               C^C[AT][AT]GG
             2419  CCTTGG              
TaqI          409  TCGA                 T^CGA
             2664  TCGA                
TthHB8I       409  TCGA                 T^CGA
             2664  TCGA                
XmnI         1397  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1577  GAATGTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanI        BanII       
BbvI        BfaI        BglII       BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII