##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AclI        AflII       AgeI        AhdI        Aor51HI     
AscI        AseI        AsnI        Asp700I     AspEI       
AviII       AvrII       BalI        BbeI        BbrPI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsiEI       
BsiWI       BspDI       BspHI       BspLU11I    BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     CelII       
ClaI        DraI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        HincII      
HindII      HpaI        KasI        MamI        MfeI        
MluI        MluNI       MscI        MunI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII        1641  GACGTC               GACGT^C
Acc65I       2562  GGTACC               G^GTACC
AccI         1782  GTCTAC               GT^[AC][GT]AC
AccIII        316  TCCGGA               T^CCGGA
AcyI         1641  GACGTC               G[GA]^CG[CT]C
AflIII       1426  ACACGT               A^C[GA][CT]GT
Alw44I        887  GTGCAC               G^TGCAC
ApaLI         887  GTGCAC               G^TGCAC
Asp718I      2562  GGTACC               G^GTACC
AspI         2204  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        3057  CCTCAGC              CCTCAGC(-5/-2)
BcgI          240  GCAGAGCTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         353  GGATAC               GTATCC(6/5)
BsaHI        1641  GACGTC               G[GA]^CG[CT]C
BsaI          684  GAGACC               GGTCTC(1/5)
BseAI         316  TCCGGA               T^CCGGA
Bsp1407I      769  TGTACA               T^GTACA
BspEI         316  TCCGGA               T^CCGGA
BspMI        1962  GCAGGT               ACCTGC(4/8)
BsrGI         769  TGTACA               T^GTACA
BstAPI       1925  GCATGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        809  GGTGACC              G^GT[ACGT]ACC
BstPI         809  GGTGACC              G^GT[ACGT]ACC
CpoI           20  CGGTCCG              CG^G[AT]CCG
DrdI          645  GACCTCAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       809  GGTGACC              G^GT[ACGT]ACC
EcoRI         279  GAATTC               G^AATTC
HaeII        2344  GGCGCT               [GA]GCGC^[CT]
Hin1I        1641  GACGTC               G[GA]^CG[CT]C
HindIII      3098  AAGCTT               A^AGCTT
KpnI         2562  GGTACC               GGTAC^C
MroI          316  TCCGGA               T^CCGGA
PflFI        2204  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1164  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        3051  CAGCTG               CAG^CTG
RsrII          20  CGGTCCG              CG^G[AT]CCG
SacI          243  GAGCTC               GAGCT^C
SapI         2499  GAAGAGC              GCTCTTC(1/4)
SmaI           99  CCCGGG               CCC^GGG
SspBI         769  TGTACA               T^GTACA
Tth111I      2204  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          379  CCATGAGGCTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          99  CCCGGG               C^CCGGG
XmaI           99  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwNI        1012  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1806  CAGCACCTG           
BamHI         319  GGATCC               G^GATCC
             1112  GGATCC              
BanI          104  GGCACC               G^G[CT][GA]CC
             2562  GGTACC              
BsaWI         316  TCCGGA               [AT]^CCGG[AT]
              367  ACCGGA              
BsgI          476  GTGCAG               GTGCAG(16/14)
              495  GTGCAG              
BsmBI        1182  GAGACG               CGTCTC(1/5)
             2881  GAGACG              
BsmI         1828  GCATTC               GAATGC(1/-1)
             2048  GCATTC              
BsrBI        1472  CCGCTC               CCGCTC(-3/-3)
             1107  GAGCGG              
BsrDI        2586  CATTGC               GCAATG(2/0)
             2263  GCAATG              
BsrFI           8  GCCGGC               [GA]^CCGG[CT]
               77  GCCGGC              
BstXI         622  CCATCTCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2164  CCAAATACTTGG        
Cfr10I          8  GCCGGC               [GA]^CCGG[CT]
               77  GCCGGC              
KspI          108  CCGCGG               CCGC^GG
              118  CCGCGG              
NaeI            8  GCCGGC               GCC^GGC
               77  GCCGGC              
NgoMIV          8  GCCGGC               G^CCGGC
               77  GCCGGC              
PpuMI         652  GGGTCCT              [GA]G^G[AT]CC[CT]
             2696  GGGTCCT             
SacII         108  CCGCGG               CCGC^GG
              118  CCGCGG              
SexAI        1810  ACCTGGT              A^CC[AT]GGT
             2611  ACCAGGT             
TfiI          290  GAATC                G^A[AT]TC
              983  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AocI        ApaI        ApoI        AspHI       
AvaI        AvaII       BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PaeR7I      PflMI       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoI        XhoII