################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BbeI BbrPI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsiEI BsiWI BspDI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI DraI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HpaI KasI MamI MfeI MluI MluNI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 1641 GACGTC GACGT^C Acc65I 2562 GGTACC G^GTACC AccI 1782 GTCTAC GT^[AC][GT]AC AccIII 316 TCCGGA T^CCGGA AcyI 1641 GACGTC G[GA]^CG[CT]C AflIII 1426 ACACGT A^C[GA][CT]GT Alw44I 887 GTGCAC G^TGCAC ApaLI 887 GTGCAC G^TGCAC Asp718I 2562 GGTACC G^GTACC AspI 2204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 3057 CCTCAGC CCTCAGC(-5/-2) BcgI 240 GCAGAGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 353 GGATAC GTATCC(6/5) BsaHI 1641 GACGTC G[GA]^CG[CT]C BsaI 684 GAGACC GGTCTC(1/5) BseAI 316 TCCGGA T^CCGGA Bsp1407I 769 TGTACA T^GTACA BspEI 316 TCCGGA T^CCGGA BspMI 1962 GCAGGT ACCTGC(4/8) BsrGI 769 TGTACA T^GTACA BstAPI 1925 GCATGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 809 GGTGACC G^GT[ACGT]ACC BstPI 809 GGTGACC G^GT[ACGT]ACC CpoI 20 CGGTCCG CG^G[AT]CCG DrdI 645 GACCTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 809 GGTGACC G^GT[ACGT]ACC EcoRI 279 GAATTC G^AATTC HaeII 2344 GGCGCT [GA]GCGC^[CT] Hin1I 1641 GACGTC G[GA]^CG[CT]C HindIII 3098 AAGCTT A^AGCTT KpnI 2562 GGTACC GGTAC^C MroI 316 TCCGGA T^CCGGA PflFI 2204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1164 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 3051 CAGCTG CAG^CTG RsrII 20 CGGTCCG CG^G[AT]CCG SacI 243 GAGCTC GAGCT^C SapI 2499 GAAGAGC GCTCTTC(1/4) SmaI 99 CCCGGG CCC^GGG SspBI 769 TGTACA T^GTACA Tth111I 2204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 379 CCATGAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 99 CCCGGG C^CCGGG XmaI 99 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwNI 1012 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 1806 CAGCACCTG BamHI 319 GGATCC G^GATCC 1112 GGATCC BanI 104 GGCACC G^G[CT][GA]CC 2562 GGTACC BsaWI 316 TCCGGA [AT]^CCGG[AT] 367 ACCGGA BsgI 476 GTGCAG GTGCAG(16/14) 495 GTGCAG BsmBI 1182 GAGACG CGTCTC(1/5) 2881 GAGACG BsmI 1828 GCATTC GAATGC(1/-1) 2048 GCATTC BsrBI 1472 CCGCTC CCGCTC(-3/-3) 1107 GAGCGG BsrDI 2586 CATTGC GCAATG(2/0) 2263 GCAATG BsrFI 8 GCCGGC [GA]^CCGG[CT] 77 GCCGGC BstXI 622 CCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2164 CCAAATACTTGG Cfr10I 8 GCCGGC [GA]^CCGG[CT] 77 GCCGGC KspI 108 CCGCGG CCGC^GG 118 CCGCGG NaeI 8 GCCGGC GCC^GGC 77 GCCGGC NgoMIV 8 GCCGGC G^CCGGC 77 GCCGGC PpuMI 652 GGGTCCT [GA]G^G[AT]CC[CT] 2696 GGGTCCT SacII 108 CCGCGG CCGC^GG 118 CCGCGG SexAI 1810 ACCTGGT A^CC[AT]GGT 2611 ACCAGGT TfiI 290 GAATC G^A[AT]TC 983 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AocI ApaI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PaeR7I PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoI XhoII