##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp718I
AspI AviII BamHI BbrPI BcgI
BciVI BclI BfrI BglII BsaAI
BsaBI BsiEI BsiWI BspDI BssHII
Bst1107I BstZ17I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MseI MunI NdeI NheI
NotI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SexAI SfiI SgrAI SnaBI SphI
SspI SwaI Tru9I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1518 GACGTC GACGT^C
AccIII 417 TCCGGA T^CCGGA
AcsI 2063 GAATTT [GA]^AATT[CT]
AflIII 874 ACATGT A^C[GA][CT]GT
AhdI 3151 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2063 GAATTT [GA]^AATT[CT]
Asp700I 437 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3151 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 86 CCTAGG C^CTAGG
BbeI 1584 GGCGCC GGCGC^C
BbvCI 1727 CCTCAGC CCTCAGC(-5/-2)
BlnI 86 CCTAGG C^CTAGG
BlpI 204 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 204 GCTCAGC GC^T[ACGT]AGC
BseAI 417 TCCGGA T^CCGGA
BsmI 632 GAATGC GAATGC(1/-1)
Bsp1407I 394 TGTACA T^GTACA
BspEI 417 TCCGGA T^CCGGA
BspHI 125 TCATGA T^CATGA
BspLU11I 874 ACATGT A^CATGT
BsrBI 882 GAGCGG CCGCTC(-3/-3)
BsrGI 394 TGTACA T^GTACA
BssSI 2169 CACGAG CACGAG(-5/-1)
BstBI 895 TTCGAA TT^CGAA
CelII 204 GCTCAGC GC^T[ACGT]AGC
Eam1105I 3151 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KasI 1584 GGCGCC G^GCGCC
MroI 417 TCCGGA T^CCGGA
NarI 1584 GGCGCC GG^CGCC
NruI 725 TCGCGA TCG^CGA
NspV 895 TTCGAA TT^CGAA
PaeR7I 1407 CTCGAG C^TCGAG
RcaI 125 TCATGA T^CATGA
ScaI 586 AGTACT AGT^ACT
SfoI 1584 GGCGCC GGC^GCC
SfuI 895 TTCGAA TT^CGAA
SmaI 808 CCCGGG CCC^GGG
SmlI 1407 CTCGAG C^T[CT][GA]AG
SpeI 31 ACTAGT A^CTAGT
SspBI 394 TGTACA T^GTACA
XhoI 1407 CTCGAG C^TCGAG
XmaCI 808 CCCGGG C^CCGGG
XmaI 808 CCCGGG C^CCGGG
XmnI 437 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 471 CGCG CG^CG
726 CGCG
AcyI 1518 GACGTC G[GA]^CG[CT]C
1584 GGCGCC
Alw44I 1341 GTGCAC G^TGCAC
2166 GTGCAC
ApaLI 1341 GTGCAC G^TGCAC
2166 GTGCAC
AvaI 808 CCCGGG C^[CT]CG[GA]G
1407 CTCGAG
BglI 2191 GCCTGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3092 GCCCCTTTGGC
BsaHI 1518 GACGTC G[GA]^CG[CT]C
1584 GGCGCC
BsaWI 417 TCCGGA [AT]^CCGG[AT]
1030 ACCGGA
BsoBI 808 CCCGGG C^[CT]CG[GA]G
1407 CTCGAG
BsrDI 60 CATTGC GCAATG(2/0)
1061 CATTGC
BstUI 471 CGCG CG^CG
726 CGCG
HaeII 801 GGCGCT [GA]GCGC^[CT]
1584 GGCGCC
HgaI 1417 GCGTC GACGC(5/10)
2130 GCGTC
Hin1I 1518 GACGTC G[GA]^CG[CT]C
1584 GGCGCC
MvnI 471 CGCG CG^CG
726 CGCG
NcoI 2481 CCATGG C^CATGG
2772 CCATGG
SacI 2379 GAGCTC GAGCT^C
2476 GAGCTC
SbfI 1547 CCTGCAGG CCTGCA^GG
2090 CCTGCAGG
Sse8387I 1547 CCTGCAGG CCTGCA^GG
2090 CCTGCAGG
Tsp509I 342 AATT ^AATT
2064 AATT
XcmI 962 CCATGCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1570 CCAAGGGCGTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XhoII