##################################
# NO CUT ENZYMES (   53 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        AscI        
AviII       BbrPI       BfrI        BsaAI       BsaBI       
BseAI       BsiWI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstZ17I     
ClaI        CpoI        DraIII      EcoT22I     FspI        
HpaI        MamI        MfeI        MroI        MunI        
NheI        NotI        NsiI        PacI        PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SalI        SbfI        ScaI        SexAI       
SfiI        SgrAI       SnaBI       SphI        Sse8387I    
SspBI       SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Alw44I        707  GTGCAC               G^TGCAC
Aor51HI      3108  AGCGCT               AGC^GCT
ApaLI         707  GTGCAC               G^TGCAC
AseI         1430  ATTAAT               AT^TAAT
AsnI         1430  ATTAAT               AT^TAAT
AspI         4805  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        5001  CCTAGG               C^CTAGG
BclI         3936  TGATCA               T^GATCA
BglII        4918  AGATCT               A^GATCT
BlnI         5001  CCTAGG               C^CTAGG
BlpI         4112  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     4112  GCTCAGC              GC^T[ACGT]AGC
BsaWI        4011  TCCGGT               [AT]^CCGG[AT]
BsiEI         531  CGGCCG               CG[GA][CT]^CG
BspHI        4365  TCATGA               T^CATGA
BssSI        3144  CACGAG               CACGAG(-5/-1)
BstEII       5046  GGTGACC              G^GT[ACGT]ACC
BstPI        5046  GGTGACC              G^GT[ACGT]ACC
CelII        4112  GCTCAGC              GC^T[ACGT]AGC
DrdI         1647  GACAAAAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          531  CGGCCG               C^GGCCG
EclXI         531  CGGCCG               C^GGCCG
Eco47III     3108  AGCGCT               AGC^GCT
Eco52I        531  CGGCCG               C^GGCCG
EcoNI        5180  CCTGAGGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      5046  GGTGACC              G^GT[ACGT]ACC
EcoRV        4304  GATATC               GAT^ATC
FbaI         3936  TGATCA               T^GATCA
FseI          528  GGCCGGCC             GGCCGG^CC
HincII       1925  GTCAAC               GT[CT]^[GA]AC
HindII       1925  GTCAAC               GT[CT]^[GA]AC
KspI          758  CCGCGG               CCGC^GG
MluI          711  ACGCGT               A^CGCGT
NdeI         1505  CATATG               CA^TATG
NruI          549  TCGCGA               TCG^CGA
NspI         1752  GCATGT               [GA]CATG^[CT]
PaeR7I       2406  CTCGAG               C^TCGAG
PflFI        4805  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1337  GACATCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1430  ATTAAT               AT^TAAT
RcaI         4365  TCATGA               T^CATGA
SacII         758  CCGCGG               CCGC^GG
SpeI         2638  ACTAGT               A^CTAGT
SspI         5081  AATATT               AAT^ATT
Tth111I      4805  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2406  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

Acc65I       2800  GGTACC               G^GTACC
             4770  GGTACC              
AccI         2260  GTCTAC               GT^[AC][GT]AC
             2551  GTCTAC              
Asp700I      3276  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4249  GAACTTCTTC          
Asp718I      2800  GGTACC               G^GTACC
             4770  GGTACC              
BamHI        2608  GGATCC               G^GATCC
             3390  GGATCC              
BbeI           97  GGCGCC               GGCGC^C
             3651  GGCGCC              
BbvCI          22  GCTGAGG              CCTCAGC(-5/-2)
             5296  GCTGAGG             
BglI          415  GCCGACTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              533  GCCGCTCCGGC         
BsmBI         381  CGTCTC               CGTCTC(1/5)
             1175  CGTCTC              
BsmI          359  GAATGC               GAATGC(1/-1)
             1703  GCATTC              
BsrFI         529  GCCGGC               [GA]^CCGG[CT]
             3648  GCCGGC              
BstBI        1008  TTCGAA               TT^CGAA
             5058  TTCGAA              
BstXI        4059  CCAATCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4680  CCAGAGGTTTGG        
Cfr10I        529  GCCGGC               [GA]^CCGG[CT]
             3648  GCCGGC              
DraI         5102  TTTAAA               TTT^AAA
             5426  TTTAAA              
HindIII      2117  AAGCTT               A^AGCTT
             4661  AAGCTT              
KasI           97  GGCGCC               G^GCGCC
             3651  GGCGCC              
KpnI         2800  GGTACC               GGTAC^C
             4770  GGTACC              
NaeI          529  GCCGGC               GCC^GGC
             3648  GCCGGC              
NarI           97  GGCGCC               GG^CGCC
             3651  GGCGCC              
NgoMIV        529  GCCGGC               G^CCGGC
             3648  GCCGGC              
NspV         1008  TTCGAA               TT^CGAA
             5058  TTCGAA              
SfoI           97  GGCGCC               GGC^GCC
             3651  GGCGCC              
SfuI         1008  TTCGAA               TT^CGAA
             5058  TTCGAA              
SmaI          509  CCCGGG               CCC^GGG
             2544  CCCGGG              
StuI         3874  AGGCCT               AGG^CCT
             4513  AGGCCT              
XmaCI         509  CCCGGG               C^CCGGG
             2544  CCCGGG              
XmaI          509  CCCGGG               C^CCGGG
             2544  CCCGGG              
XmnI         3276  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4249  GAACTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

AatII       AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AhdI        AluI        AlwI        
AlwNI       AocI        ApaI        ApoI        AspEI       
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BcgI        BciVI       
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        Eam1105I    EarI        Eco81I      EcoO109I    
EcoRI       EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII