################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AccIII AclI AflII AgeI AscI AviII BbrPI BfrI BsaAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstZ17I ClaI CpoI DraIII EcoT22I FspI HpaI MamI MfeI MroI MunI NheI NotI NsiI PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SgrAI SnaBI SphI Sse8387I SspBI SwaI XbaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Alw44I 707 GTGCAC G^TGCAC Aor51HI 3108 AGCGCT AGC^GCT ApaLI 707 GTGCAC G^TGCAC AseI 1430 ATTAAT AT^TAAT AsnI 1430 ATTAAT AT^TAAT AspI 4805 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 5001 CCTAGG C^CTAGG BclI 3936 TGATCA T^GATCA BglII 4918 AGATCT A^GATCT BlnI 5001 CCTAGG C^CTAGG BlpI 4112 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4112 GCTCAGC GC^T[ACGT]AGC BsaWI 4011 TCCGGT [AT]^CCGG[AT] BsiEI 531 CGGCCG CG[GA][CT]^CG BspHI 4365 TCATGA T^CATGA BssSI 3144 CACGAG CACGAG(-5/-1) BstEII 5046 GGTGACC G^GT[ACGT]ACC BstPI 5046 GGTGACC G^GT[ACGT]ACC CelII 4112 GCTCAGC GC^T[ACGT]AGC DrdI 1647 GACAAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 531 CGGCCG C^GGCCG EclXI 531 CGGCCG C^GGCCG Eco47III 3108 AGCGCT AGC^GCT Eco52I 531 CGGCCG C^GGCCG EcoNI 5180 CCTGAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 5046 GGTGACC G^GT[ACGT]ACC EcoRV 4304 GATATC GAT^ATC FbaI 3936 TGATCA T^GATCA FseI 528 GGCCGGCC GGCCGG^CC HincII 1925 GTCAAC GT[CT]^[GA]AC HindII 1925 GTCAAC GT[CT]^[GA]AC KspI 758 CCGCGG CCGC^GG MluI 711 ACGCGT A^CGCGT NdeI 1505 CATATG CA^TATG NruI 549 TCGCGA TCG^CGA NspI 1752 GCATGT [GA]CATG^[CT] PaeR7I 2406 CTCGAG C^TCGAG PflFI 4805 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1337 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1430 ATTAAT AT^TAAT RcaI 4365 TCATGA T^CATGA SacII 758 CCGCGG CCGC^GG SpeI 2638 ACTAGT A^CTAGT SspI 5081 AATATT AAT^ATT Tth111I 4805 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2406 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 2800 GGTACC G^GTACC 4770 GGTACC AccI 2260 GTCTAC GT^[AC][GT]AC 2551 GTCTAC Asp700I 3276 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4249 GAACTTCTTC Asp718I 2800 GGTACC G^GTACC 4770 GGTACC BamHI 2608 GGATCC G^GATCC 3390 GGATCC BbeI 97 GGCGCC GGCGC^C 3651 GGCGCC BbvCI 22 GCTGAGG CCTCAGC(-5/-2) 5296 GCTGAGG BglI 415 GCCGACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 533 GCCGCTCCGGC BsmBI 381 CGTCTC CGTCTC(1/5) 1175 CGTCTC BsmI 359 GAATGC GAATGC(1/-1) 1703 GCATTC BsrFI 529 GCCGGC [GA]^CCGG[CT] 3648 GCCGGC BstBI 1008 TTCGAA TT^CGAA 5058 TTCGAA BstXI 4059 CCAATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4680 CCAGAGGTTTGG Cfr10I 529 GCCGGC [GA]^CCGG[CT] 3648 GCCGGC DraI 5102 TTTAAA TTT^AAA 5426 TTTAAA HindIII 2117 AAGCTT A^AGCTT 4661 AAGCTT KasI 97 GGCGCC G^GCGCC 3651 GGCGCC KpnI 2800 GGTACC GGTAC^C 4770 GGTACC NaeI 529 GCCGGC GCC^GGC 3648 GCCGGC NarI 97 GGCGCC GG^CGCC 3651 GGCGCC NgoMIV 529 GCCGGC G^CCGGC 3648 GCCGGC NspV 1008 TTCGAA TT^CGAA 5058 TTCGAA SfoI 97 GGCGCC GGC^GCC 3651 GGCGCC SfuI 1008 TTCGAA TT^CGAA 5058 TTCGAA SmaI 509 CCCGGG CCC^GGG 2544 CCCGGG StuI 3874 AGGCCT AGG^CCT 4513 AGGCCT XmaCI 509 CCCGGG C^CCGGG 2544 CCCGGG XmaI 509 CCCGGG C^CCGGG 2544 CCCGGG XmnI 3276 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4249 GAACTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AatII AccII AciI AcsI AcyI AfaI AflIII AhdI AluI AlwI AlwNI AocI ApaI ApoI AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BciVI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII