##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       Acc65I      AccIII      AgeI        AhdI        
Alw44I      AocI        ApaI        ApaLI       AscI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BbeI        BbvCI       BcnI        BglII       BlnI        
BlpI        Bpu1102I    BseAI       BseRI       BsiEI       
BsiWI       Bsp1407I    BspEI       BsrBI       BsrGI       
BssHII      BssSI       BstAPI      BstBI       BstEII      
BstPI       Bsu36I      CelII       CpoI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoO65I     
EcoT22I     FseI        FspI        HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NciI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PvuI        RsrII       SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       StuI        SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         4438  GTATAC               GT^[AC][GT]AC
AccII         292  CGCG                 CG^CG
AclI         3386  AACGTT               AA^CGTT
AcyI         1528  GGCGTC               G[GA]^CG[CT]C
AvaI         2018  CTCGGG               C^[CT]CG[GA]G
BalI          143  TGGCCA               TGG^CCA
BanI         3973  GGCACC               G^G[CT][GA]CC
BanII          20  GAGCTC               G[GA]GC[CT]^C
BbrPI        1243  CACGTG               CAC^GTG
BglI         3502  GCCTTTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1243  CACGTG               [CT]AC^GT[GA]
BsaBI        3286  GATGCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1528  GGCGTC               G[GA]^CG[CT]C
BsaI         4241  GGTCTC               GGTCTC(1/5)
BsmBI        1634  CGTCTC               CGTCTC(1/5)
BsoBI        2018  CTCGGG               C^[CT]CG[GA]G
BspDI        1852  ATCGAT               AT^CGAT
BsrFI        3046  ACCGGC               [GA]^CCGG[CT]
Bst1107I     4438  GTATAC               GTA^TAC
BstUI         292  CGCG                 CG^CG
BstXI         782  CCATTGAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        3372  AGATCC               [GA]^GATC[CT]
BstZ17I      4438  GTATAC               GTA^TAC
Cfr10I       3046  ACCGGC               [GA]^CCGG[CT]
ClaI         1852  ATCGAT               AT^CGAT
DraIII       4122  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3403  GACCAGAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          143  TGGCCA               [CT]^GGCC[GA]
EcoRV        1849  GATATC               GAT^ATC
Hin1I        1528  GGCGTC               G[GA]^CG[CT]C
MamI         3286  GATGCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         3372  AGATCC               [GA]^GATC[CT]
MluNI         143  TGGCCA               TGG^CCA
MscI          143  TGGCCA               TGG^CCA
MspA1I       1935  CAGCTG               C[AC]G^C[GT]G
MvnI          292  CGCG                 CG^CG
NdeI         3415  CATATG               CA^TATG
NheI         1446  GCTAGC               G^CTAGC
PflMI         988  CCAAATAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1243  CACGTG               CAC^GTG
PmlI         1243  CACGTG               CAC^GTG
PshAI         476  GACGGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3386  AACGTT               AA^CGTT
PvuII        1935  CAGCTG               CAG^CTG
SacI           20  GAGCTC               GAGCT^C
Van91I        988  CCAAATAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          782  CCATTGAGTTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        3372  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AflII         879  CTTAAG               C^TTAAG
             2542  CTTAAG              
Aor51HI       569  AGCGCT               AGC^GCT
             3920  AGCGCT              
AspHI          20  GAGCTC               G[AT]GC[AT]^C
             3990  GAGCAC              
AspI         1518  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1958  GACATGGTC           
BcgI          337  GCAGTGACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1320  GCAGACACCTCG        
BclI         1467  TGATCA               T^GATCA
             3123  TGATCA              
BfrI          879  CTTAAG               C^TTAAG
             2542  CTTAAG              
BsaWI        1863  ACCGGA               [AT]^CCGG[AT]
             3482  TCCGGT              
BsgI          248  GTGCAG               GTGCAG(16/14)
             3690  GTGCAG              
BsiHKAI        20  GAGCTC               G[AT]GC[AT]^C
             3990  GAGCAC              
BsmI         2400  GAATGC               GAATGC(1/-1)
             4204  GAATGC              
CfoI          570  GCGC                 GCG^C
             3921  GCGC                
DsaI          158  CCATGG               C^C[GA][CT]GG
             4279  CCATGG              
Eco47III      569  AGCGCT               AGC^GCT
             3920  AGCGCT              
EcoRI        1158  GAATTC               G^AATTC
             3881  GAATTC              
FauI           55  CCCGC                CCCGC(4/6)
              293  GCGGG               
FbaI         1467  TGATCA               T^GATCA
             3123  TGATCA              
HaeII         569  AGCGCT               [GA]GCGC^[CT]
             3920  AGCGCT              
HhaI          570  GCGC                 GCG^C
             3921  GCGC                
HinP1I        570  GCGC                 G^CGC
             3921  GCGC                
HindIII      2167  AAGCTT               A^AGCTT
             3202  AAGCTT              
NcoI          158  CCATGG               C^CATGG
             4279  CCATGG              
PflFI        1518  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1958  GACATGGTC           
PpuMI        2701  AGGTCCT              [GA]G^G[AT]CC[CT]
             4250  GGGACCC             
ScaI         2098  AGTACT               AGT^ACT
             2761  AGTACT              
Tth111I      1518  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1958  GACATGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AseI        AsnI        
Asp700I     AvaII       BbsI        BbvI        BciVI       
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspHI       BspLU11I    BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraI        DraII       EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PshBI       PstI        RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SpeI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XmnI