################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BamHI BbeI BbvCI BcnI BglII BlnI BlpI Bpu1102I BseAI BseRI BsiEI BsiWI Bsp1407I BspEI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI Bsu36I CelII CpoI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoT22I FseI FspI HincII HindII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NciI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 4438 GTATAC GT^[AC][GT]AC AccII 292 CGCG CG^CG AclI 3386 AACGTT AA^CGTT AcyI 1528 GGCGTC G[GA]^CG[CT]C AvaI 2018 CTCGGG C^[CT]CG[GA]G BalI 143 TGGCCA TGG^CCA BanI 3973 GGCACC G^G[CT][GA]CC BanII 20 GAGCTC G[GA]GC[CT]^C BbrPI 1243 CACGTG CAC^GTG BglI 3502 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1243 CACGTG [CT]AC^GT[GA] BsaBI 3286 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1528 GGCGTC G[GA]^CG[CT]C BsaI 4241 GGTCTC GGTCTC(1/5) BsmBI 1634 CGTCTC CGTCTC(1/5) BsoBI 2018 CTCGGG C^[CT]CG[GA]G BspDI 1852 ATCGAT AT^CGAT BsrFI 3046 ACCGGC [GA]^CCGG[CT] Bst1107I 4438 GTATAC GTA^TAC BstUI 292 CGCG CG^CG BstXI 782 CCATTGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 3372 AGATCC [GA]^GATC[CT] BstZ17I 4438 GTATAC GTA^TAC Cfr10I 3046 ACCGGC [GA]^CCGG[CT] ClaI 1852 ATCGAT AT^CGAT DraIII 4122 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3403 GACCAGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 143 TGGCCA [CT]^GGCC[GA] EcoRV 1849 GATATC GAT^ATC Hin1I 1528 GGCGTC G[GA]^CG[CT]C MamI 3286 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 3372 AGATCC [GA]^GATC[CT] MluNI 143 TGGCCA TGG^CCA MscI 143 TGGCCA TGG^CCA MspA1I 1935 CAGCTG C[AC]G^C[GT]G MvnI 292 CGCG CG^CG NdeI 3415 CATATG CA^TATG NheI 1446 GCTAGC G^CTAGC PflMI 988 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1243 CACGTG CAC^GTG PmlI 1243 CACGTG CAC^GTG PshAI 476 GACGGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 3386 AACGTT AA^CGTT PvuII 1935 CAGCTG CAG^CTG SacI 20 GAGCTC GAGCT^C Van91I 988 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 782 CCATTGAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 3372 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflII 879 CTTAAG C^TTAAG 2542 CTTAAG Aor51HI 569 AGCGCT AGC^GCT 3920 AGCGCT AspHI 20 GAGCTC G[AT]GC[AT]^C 3990 GAGCAC AspI 1518 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1958 GACATGGTC BcgI 337 GCAGTGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1320 GCAGACACCTCG BclI 1467 TGATCA T^GATCA 3123 TGATCA BfrI 879 CTTAAG C^TTAAG 2542 CTTAAG BsaWI 1863 ACCGGA [AT]^CCGG[AT] 3482 TCCGGT BsgI 248 GTGCAG GTGCAG(16/14) 3690 GTGCAG BsiHKAI 20 GAGCTC G[AT]GC[AT]^C 3990 GAGCAC BsmI 2400 GAATGC GAATGC(1/-1) 4204 GAATGC CfoI 570 GCGC GCG^C 3921 GCGC DsaI 158 CCATGG C^C[GA][CT]GG 4279 CCATGG Eco47III 569 AGCGCT AGC^GCT 3920 AGCGCT EcoRI 1158 GAATTC G^AATTC 3881 GAATTC FauI 55 CCCGC CCCGC(4/6) 293 GCGGG FbaI 1467 TGATCA T^GATCA 3123 TGATCA HaeII 569 AGCGCT [GA]GCGC^[CT] 3920 AGCGCT HhaI 570 GCGC GCG^C 3921 GCGC HinP1I 570 GCGC G^CGC 3921 GCGC HindIII 2167 AAGCTT A^AGCTT 3202 AAGCTT NcoI 158 CCATGG C^CATGG 4279 CCATGG PflFI 1518 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1958 GACATGGTC PpuMI 2701 AGGTCCT [GA]G^G[AT]CC[CT] 4250 GGGACCC ScaI 2098 AGTACT AGT^ACT 2761 AGTACT Tth111I 1518 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1958 GACATGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AvaII BbsI BbvI BciVI BfaI BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI