################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AviII AvrII BamHI BanI BanII BbeI BbrPI BcnI BglI BglII BlnI BmyI BpmI BsaHI BsaI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspDI BspEI BsrBI BsrFI BssSI Bst1107I BstEII BstPI BstXI BstYI BstZ17I Cfr10I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoT14I FseI FspI HaeII Hin1I KasI KpnI MflI MluI MroI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I StuI StyI SwaI Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflII 1089 CTTAAG C^TTAAG AlwNI 130 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 699 CCCGAG C^[CT]CG[GA]G BbvCI 65 CCTCAGC CCTCAGC(-5/-2) BfrI 1089 CTTAAG C^TTAAG BlpI 275 GCTAAGC GC^T[ACGT]AGC Bpu1102I 275 GCTAAGC GC^T[ACGT]AGC BsaWI 1469 ACCGGA [AT]^CCGG[AT] BseRI 2670 GAGGAG GAGGAG(10/8) BsoBI 699 CCCGAG C^[CT]CG[GA]G BspLU11I 2408 ACATGT A^CATGT BspMI 1796 ACCTGC ACCTGC(4/8) BsrDI 3931 CATTGC GCAATG(2/0) BssHII 70 GCGCGC G^CGCGC BstAPI 2100 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 869 TTCGAA TT^CGAA CelII 275 GCTAAGC GC^T[ACGT]AGC EcoNI 3645 CCTTTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 694 GAATTC G^AATTC EcoRV 687 GATATC GAT^ATC EcoT22I 2983 ATGCAT ATGCA^T HapII 1470 CCGG C^CGG HincII 2724 GTTAAC GT[CT]^[GA]AC HindII 2724 GTTAAC GT[CT]^[GA]AC HpaI 2724 GTTAAC GTT^AAC HpaII 1470 CCGG C^CGG KspI 53 CCGCGG CCGC^GG MfeI 4131 CAATTG C^AATTG MspI 1470 CCGG C^CGG MunI 4131 CAATTG C^AATTG NsiI 2983 ATGCAT ATGCA^T NspV 869 TTCGAA TT^CGAA SacII 53 CCGCGG CCGC^GG SfuI 869 TTCGAA TT^CGAA SpeI 105 ACTAGT A^CTAGT XcmI 3244 CCATAATACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 54 CGCG CG^CG 71 CGCG AflIII 2408 ACATGT A^C[GA][CT]GT 3997 ACGTGT AspI 294 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2179 GACAGTGTC BcgI 2090 CGATAAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3233 CGAGGGCTATGC BciVI 58 GGATAC GTATCC(6/5) 2224 GGATAC BclI 1253 TGATCA T^GATCA 2198 TGATCA BsaAI 838 TACGTG [CT]AC^GT[GA] 3996 TACGTG BsaBI 897 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2760 GATCTTGATC BsmAI 1664 GAGAC GTCTC(1/5) 425 GTCTC Bsp1407I 2900 TGTACA T^GTACA 3638 TGTACA BsrGI 2900 TGTACA T^GTACA 3638 TGTACA BstUI 54 CGCG CG^CG 71 CGCG DraIII 2581 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 3479 CACAAAGTG DsaI 53 CCGCGG C^C[GA][CT]GG 562 CCACGG EarI 2716 CTCTTC CTCTTC(1/4) 872 GAAGAG FauI 52 CCCGC CCCGC(4/6) 55 GCGGG FbaI 1253 TGATCA T^GATCA 2198 TGATCA Ksp632I 2716 CTCTTC CTCTTC(1/4) 872 GAAGAG MamI 897 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2760 GATCTTGATC MvnI 54 CGCG CG^CG 71 CGCG PflFI 294 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2179 GACAGTGTC PvuII 861 CAGCTG CAG^CTG 3061 CAGCTG ScaI 2847 AGTACT AGT^ACT 3140 AGTACT SspBI 2900 TGTACA T^GTACA 3638 TGTACA Tth111I 294 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2179 GACAGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AocI ApoI AseI AsnI Asp700I AvaII BalI BbsI BbvI BfaI BpuAI BsaJI BsgI BsiYI BslI BsmFI BsmI BspHI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI Eco81I EcoRII Fnu4HI FokI HaeIII HgaI HhaI HinP1I HindIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI