##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AspHI       AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BcnI        BglI        
BglII       BlnI        BmyI        BpmI        BsaHI       
BsaI        BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       Bsp1286I    BspDI       BspEI       BsrBI       
BsrFI       BssSI       Bst1107I    BstEII      BstPI       
BstXI       BstYI       BstZ17I     Cfr10I      ClaI        
CpoI        DraII       DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO109I    EcoO65I     
EcoT14I     FseI        FspI        HaeII       Hin1I       
KasI        KpnI        MflI        MluI        MroI        
NaeI        NarI        NciI        NcoI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        RsrII       
SacI        SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    StuI        StyI        SwaI        
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AflII        1089  CTTAAG               C^TTAAG
AlwNI         130  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          699  CCCGAG               C^[CT]CG[GA]G
BbvCI          65  CCTCAGC              CCTCAGC(-5/-2)
BfrI         1089  CTTAAG               C^TTAAG
BlpI          275  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      275  GCTAAGC              GC^T[ACGT]AGC
BsaWI        1469  ACCGGA               [AT]^CCGG[AT]
BseRI        2670  GAGGAG               GAGGAG(10/8)
BsoBI         699  CCCGAG               C^[CT]CG[GA]G
BspLU11I     2408  ACATGT               A^CATGT
BspMI        1796  ACCTGC               ACCTGC(4/8)
BsrDI        3931  CATTGC               GCAATG(2/0)
BssHII         70  GCGCGC               G^CGCGC
BstAPI       2100  GCAGATGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         869  TTCGAA               TT^CGAA
CelII         275  GCTAAGC              GC^T[ACGT]AGC
EcoNI        3645  CCTTTTGTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         694  GAATTC               G^AATTC
EcoRV         687  GATATC               GAT^ATC
EcoT22I      2983  ATGCAT               ATGCA^T
HapII        1470  CCGG                 C^CGG
HincII       2724  GTTAAC               GT[CT]^[GA]AC
HindII       2724  GTTAAC               GT[CT]^[GA]AC
HpaI         2724  GTTAAC               GTT^AAC
HpaII        1470  CCGG                 C^CGG
KspI           53  CCGCGG               CCGC^GG
MfeI         4131  CAATTG               C^AATTG
MspI         1470  CCGG                 C^CGG
MunI         4131  CAATTG               C^AATTG
NsiI         2983  ATGCAT               ATGCA^T
NspV          869  TTCGAA               TT^CGAA
SacII          53  CCGCGG               CCGC^GG
SfuI          869  TTCGAA               TT^CGAA
SpeI          105  ACTAGT               A^CTAGT
XcmI         3244  CCATAATACAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII          54  CGCG                 CG^CG
               71  CGCG                
AflIII       2408  ACATGT               A^C[GA][CT]GT
             3997  ACGTGT              
AspI          294  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2179  GACAGTGTC           
BcgI         2090  CGATAAATCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3233  CGAGGGCTATGC        
BciVI          58  GGATAC               GTATCC(6/5)
             2224  GGATAC              
BclI         1253  TGATCA               T^GATCA
             2198  TGATCA              
BsaAI         838  TACGTG               [CT]AC^GT[GA]
             3996  TACGTG              
BsaBI         897  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2760  GATCTTGATC          
BsmAI        1664  GAGAC                GTCTC(1/5)
              425  GTCTC               
Bsp1407I     2900  TGTACA               T^GTACA
             3638  TGTACA              
BsrGI        2900  TGTACA               T^GTACA
             3638  TGTACA              
BstUI          54  CGCG                 CG^CG
               71  CGCG                
DraIII       2581  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3479  CACAAAGTG           
DsaI           53  CCGCGG               C^C[GA][CT]GG
              562  CCACGG              
EarI         2716  CTCTTC               CTCTTC(1/4)
              872  GAAGAG              
FauI           52  CCCGC                CCCGC(4/6)
               55  GCGGG               
FbaI         1253  TGATCA               T^GATCA
             2198  TGATCA              
Ksp632I      2716  CTCTTC               CTCTTC(1/4)
              872  GAAGAG              
MamI          897  GATGTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2760  GATCTTGATC          
MvnI           54  CGCG                 CG^CG
               71  CGCG                
PflFI         294  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2179  GACAGTGTC           
PvuII         861  CAGCTG               CAG^CTG
             3061  CAGCTG              
ScaI         2847  AGTACT               AGT^ACT
             3140  AGTACT              
SspBI        2900  TGTACA               T^GTACA
             3638  TGTACA              
Tth111I       294  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2179  GACAGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        AseI        AsnI        Asp700I     
AvaII       BalI        BbsI        BbvI        BfaI        
BpuAI       BsaJI       BsgI        BsiYI       BslI        
BsmFI       BsmI        BspHI       BsrI        BssKI       
BstF5I      BstNI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        EaeI        
Eco81I      EcoRII      Fnu4HI      FokI        HaeIII      
HgaI        HhaI        HinP1I      HindIII     HinfI       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeI        NdeII       NlaIII      NlaIV       NspI        
PleI        PshBI       PstI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XmnI