##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BbvCI BcgI BlnI
BsaAI BsaHI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
FseI FspI HaeII HgaI Hin1I
KasI KspI MluI MroI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2656 GGTACC G^GTACC
AccII 5961 CGCG CG^CG
AflII 5298 CTTAAG C^TTAAG
ApaI 3998 GGGCCC GGGCC^C
AseI 5251 ATTAAT AT^TAAT
AsnI 5251 ATTAAT AT^TAAT
Asp718I 2656 GGTACC G^GTACC
BamHI 3072 GGATCC G^GATCC
BcnI 1120 CCGGG CC^[GC]GG
BfrI 5298 CTTAAG C^TTAAG
BglI 4854 GCCTTTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 2204 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1530 TGTACA T^GTACA
BsrBI 279 CCGCTC CCGCTC(-3/-3)
BsrGI 1530 TGTACA T^GTACA
BstUI 5961 CGCG CG^CG
CfoI 2718 GCGC GCG^C
DraIII 5411 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 250 GAATTC G^AATTC
EcoT22I 965 ATGCAT ATGCA^T
HhaI 2718 GCGC GCG^C
HinP1I 2718 GCGC G^CGC
HpaI 5112 GTTAAC GTT^AAC
KpnI 2656 GGTACC GGTAC^C
MvnI 5961 CGCG CG^CG
NciI 1120 CCGGG CC^[GC]GG
NheI 205 GCTAGC G^CTAGC
NsiI 965 ATGCAT ATGCA^T
PacI 523 TTAATTAA TTAAT^TAA
PpuMI 1043 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 5251 ATTAAT AT^TAAT
PstI 1708 CTGCAG CTGCA^G
SexAI 2753 ACCTGGT A^CC[AT]GGT
SpeI 3756 ACTAGT A^CTAGT
SphI 1314 GCATGC GCATG^C
SspBI 1530 TGTACA T^GTACA
StuI 4039 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 3837 GTAGAC GT^[AC][GT]AC
5816 GTCTAC
AocI 2118 CCTGAGG CC^T[ACGT]AGG
4344 CCTTAGG
Asp700I 1027 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2736 GAACTACTTC
AvaII 1044 GGACC G^G[AT]CC
3207 GGACC
BalI 1886 TGGCCA TGG^CCA
4230 TGGCCA
BclI 1671 TGATCA T^GATCA
5954 TGATCA
BsaBI 1785 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2368 GATACCTATC
BsaI 2291 GAGACC GGTCTC(1/5)
4629 GGTCTC
BspHI 896 TCATGA T^CATGA
3764 TCATGA
BspLU11I 2354 ACATGT A^CATGT
4613 ACATGT
BstAPI 1701 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5794 GCAGCCATTGC
Bsu36I 2118 CCTGAGG CC^T[ACGT]AGG
4344 CCTTAGG
DsaI 1862 CCATGG C^C[GA][CT]GG
3203 CCATGG
Eco81I 2118 CCTGAGG CC^T[ACGT]AGG
4344 CCTTAGG
EcoRV 2833 GATATC GAT^ATC
5743 GATATC
FbaI 1671 TGATCA T^GATCA
5954 TGATCA
MamI 1785 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2368 GATACCTATC
MluNI 1886 TGGCCA TGG^CCA
4230 TGGCCA
MscI 1886 TGGCCA TGG^CCA
4230 TGGCCA
NcoI 1862 CCATGG C^CATGG
3203 CCATGG
PflMI 1334 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3971 CCAATTGATGG
RcaI 896 TCATGA T^CATGA
3764 TCATGA
SacI 943 GAGCTC GAGCT^C
4461 GAGCTC
SspI 4293 AATATT AAT^ATT
4909 AATATT
Van91I 1334 CCAAGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3971 CCAATTGATGG
XbaI 939 TCTAGA T^CTAGA
3770 TCTAGA
XcmI 2477 CCATCTTCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3122 CCAGAGTCACTGTGG
XmnI 1027 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2736 GAACTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI BanI
BanII BbsI BbvI BciVI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII