##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspI AviII AvrII
BbrPI BfrI BlnI BsiEI BsiWI
BspDI BssHII Bst1107I BstZ17I Bsu36I
ClaI CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FseI
FspI MluI NaeI NdeI NgoMIV
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
ScaI SgrAI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1477 GTCTAC GT^[AC][GT]AC
AccIII 3500 TCCGGA T^CCGGA
AflIII 1655 ACATGT A^C[GA][CT]GT
ApaI 1144 GGGCCC GGGCC^C
BbeI 1849 GGCGCC GGCGC^C
BcgI 1638 GCAAGCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 228 TGATCA T^GATCA
BsaAI 4894 CACGTA [CT]AC^GT[GA]
BsaBI 3334 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3500 TCCGGA T^CCGGA
BspEI 3500 TCCGGA T^CCGGA
BspLU11I 1655 ACATGT A^CATGT
BstBI 4303 TTCGAA TT^CGAA
DraI 2007 TTTAAA TTT^AAA
DrdI 3787 GACAATGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4300 GAATTC G^AATTC
FbaI 228 TGATCA T^GATCA
HaeII 1849 GGCGCC [GA]GCGC^[CT]
HindIII 691 AAGCTT A^AGCTT
HpaI 3546 GTTAAC GTT^AAC
KasI 1849 GGCGCC G^GCGCC
KspI 1778 CCGCGG CCGC^GG
MamI 3334 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2340 CAATTG C^AATTG
MroI 3500 TCCGGA T^CCGGA
MunI 2340 CAATTG C^AATTG
NarI 1849 GGCGCC GG^CGCC
NheI 1540 GCTAGC G^CTAGC
NspV 4303 TTCGAA TT^CGAA
PaeR7I 3121 CTCGAG C^TCGAG
SacII 1778 CCGCGG CCGC^GG
SbfI 3343 CCTGCAGG CCTGCA^GG
SexAI 2189 ACCAGGT A^CC[AT]GGT
SfiI 570 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1849 GGCGCC GGC^GCC
SfuI 4303 TTCGAA TT^CGAA
Sse8387I 3343 CCTGCAGG CCTGCA^GG
XhoI 3121 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 156 GGTACC G^GTACC
4574 GGTACC
AccII 711 CGCG CG^CG
1779 CGCG
AhdI 2822 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5486 GACCACCTGTC
Asp718I 156 GGTACC G^GTACC
4574 GGTACC
AspEI 2822 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5486 GACCACCTGTC
AspHI 1630 GAGCTC G[AT]GC[AT]^C
5438 GAGCTC
BamHI 1310 GGATCC G^GATCC
4638 GGATCC
BciVI 700 GGATAC GTATCC(6/5)
4513 GGATAC
BlpI 24 GCTTAGC GC^T[ACGT]AGC
3477 GCTGAGC
Bpu1102I 24 GCTTAGC GC^T[ACGT]AGC
3477 GCTGAGC
BsaI 3565 GAGACC GGTCTC(1/5)
4132 GAGACC
BsiHKAI 1630 GAGCTC G[AT]GC[AT]^C
5438 GAGCTC
BsmBI 2829 CGTCTC CGTCTC(1/5)
5378 GAGACG
Bsp1407I 1658 TGTACA T^GTACA
2691 TGTACA
BspHI 3173 TCATGA T^CATGA
3890 TCATGA
BsrGI 1658 TGTACA T^GTACA
2691 TGTACA
BssSI 1759 CACGAG CACGAG(-5/-1)
5665 CTCGTG
BstEII 3141 GGTCACC G^GT[ACGT]ACC
5293 GGTTACC
BstPI 3141 GGTCACC G^GT[ACGT]ACC
5293 GGTTACC
BstUI 711 CGCG CG^CG
1779 CGCG
CelII 24 GCTTAGC GC^T[ACGT]AGC
3477 GCTGAGC
Eam1105I 2822 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5486 GACCACCTGTC
EcoO65I 3141 GGTCACC G^GT[ACGT]ACC
5293 GGTTACC
KpnI 156 GGTACC GGTAC^C
4574 GGTACC
MvnI 711 CGCG CG^CG
1779 CGCG
RcaI 3173 TCATGA T^CATGA
3890 TCATGA
SacI 1630 GAGCTC GAGCT^C
5438 GAGCTC
SapI 2560 GAAGAGC GCTCTTC(1/4)
3415 GAAGAGC
SmaI 4358 CCCGGG CCC^GGG
4523 CCCGGG
SspBI 1658 TGTACA T^GTACA
2691 TGTACA
XmaCI 4358 CCCGGG C^CCGGG
4523 CCCGGG
XmaI 4358 CCCGGG C^CCGGG
4523 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII