##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AccIII AclI AflII AgeI AscI
AseI AsnI AvrII BamHI BfrI
BlnI BsaBI BseAI BsiWI BsmBI
BsmI BspEI BspHI BssHII Bst1107I
BstBI BstZ17I DraI DrdI EcoRI
EcoRV FseI HindIII HpaI KspI
MamI MfeI MroI MunI NdeI
NotI NruI NspV PinAI PmeI
PshBI Psp1406I PvuI RcaI SacII
SfiI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 4541 GGTACC G^GTACC
AcsI 1521 AAATTC [GA]^AATT[CT]
Alw44I 3222 GTGCAC G^TGCAC
ApaLI 3222 GTGCAC G^TGCAC
ApoI 1521 AAATTC [GA]^AATT[CT]
Asp700I 1304 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4541 GGTACC G^GTACC
BclI 6082 TGATCA T^GATCA
BsaWI 6071 ACCGGA [AT]^CCGG[AT]
BspDI 5166 ATCGAT AT^CGAT
BspLU11I 358 ACATGT A^CATGT
BsrDI 1643 CATTGC GCAATG(2/0)
ClaI 5166 ATCGAT AT^CGAT
CpoI 5778 CGGACCG CG^G[AT]CCG
EcoT22I 6226 ATGCAT ATGCA^T
FbaI 6082 TGATCA T^GATCA
KpnI 4541 GGTACC GGTAC^C
MluI 944 ACGCGT A^CGCGT
NheI 2501 GCTAGC G^CTAGC
NsiI 6226 ATGCAT ATGCA^T
PacI 6337 TTAATTAA TTAAT^TAA
PaeR7I 5568 CTCGAG C^TCGAG
RsrII 5778 CGGACCG CG^G[AT]CCG
SalI 4452 GTCGAC G^TCGAC
SbfI 3206 CCTGCAGG CCTGCA^GG
SmaI 3426 CCCGGG CCC^GGG
Sse8387I 3206 CCTGCAGG CCTGCA^GG
TfiI 5914 GATTC G^A[AT]TC
XhoI 5568 CTCGAG C^TCGAG
XmaCI 3426 CCCGGG C^CCGGG
XmaI 3426 CCCGGG C^CCGGG
XmnI 1304 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 933 GACGTC GACGT^C
4419 GACGTC
AhdI 4512 GACATCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6273 GACACTGCGTC
AocI 5328 CCTGAGG CC^T[ACGT]AGG
6180 CCTCAGG
Aor51HI 3181 AGCGCT AGC^GCT
3724 AGCGCT
AspEI 4512 GACATCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6273 GACACTGCGTC
AviII 3505 TGCGCA TGC^GCA
4612 TGCGCA
BbvCI 2918 GCTGAGG CCTCAGC(-5/-2)
4215 GCTGAGG
BfaI 2502 CTAG C^TAG
3105 CTAG
BglII 1534 AGATCT A^GATCT
5926 AGATCT
BsaI 2359 GAGACC GGTCTC(1/5)
6075 GAGACC
Bsu36I 5328 CCTGAGG CC^T[ACGT]AGG
6180 CCTCAGG
Eam1105I 4512 GACATCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6273 GACACTGCGTC
Eco47III 3181 AGCGCT AGC^GCT
3724 AGCGCT
Eco81I 5328 CCTGAGG CC^T[ACGT]AGG
6180 CCTCAGG
FspI 3505 TGCGCA TGC^GCA
4612 TGCGCA
MaeI 2502 CTAG C^TAG
3105 CTAG
MseI 6337 TTAA T^TAA
6341 TTAA
ScaI 280 AGTACT AGT^ACT
1495 AGTACT
SexAI 3901 ACCTGGT A^CC[AT]GGT
4537 ACCAGGT
StuI 427 AGGCCT AGG^CCT
5257 AGGCCT
Tru9I 6337 TTAA T^TAA
6341 TTAA
Tsp509I 1522 AATT ^AATT
6339 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbrPI BbsI
BbvI BcgI BciVI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PmaCI PmlI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspBI
StyI TaqI TseI Tsp45I TspRI
Tth111I TthHB8I Van91I XcmI XhoII