##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI ApaI AscI Asp700I
Asp718I AspEI AspI AvrII BalI
BanI BbeI BciVI BcnI BfrI
BglII BlnI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BsrBI BsrDI BsrFI BssHII BstEII
BstPI BstXI Cfr10I ClaI CpoI
DraII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoRI EcoRV FseI HaeII HapII
HpaI HpaII KasI KpnI KspI
MluI MluNI MroI MscI MspI
NaeI NarI NciI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2600 GACGTC GACGT^C
AccII 911 CGCG CG^CG
AcyI 2600 GACGTC G[GA]^CG[CT]C
AseI 804 ATTAAT AT^TAAT
AsnI 804 ATTAAT AT^TAAT
AviII 2740 TGCGCA TGC^GCA
BamHI 2474 GGATCC G^GATCC
BbrPI 3281 CACGTG CAC^GTG
BclI 699 TGATCA T^GATCA
BfaI 244 CTAG C^TAG
BsaAI 3281 CACGTG [CT]AC^GT[GA]
BsaHI 2600 GACGTC G[GA]^CG[CT]C
Bsp1407I 2485 TGTACA T^GTACA
BsrGI 2485 TGTACA T^GTACA
BstAPI 2743 GCAAGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 668 TTCGAA TT^CGAA
BstUI 911 CGCG CG^CG
CfoI 2741 GCGC GCG^C
DraIII 1064 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 815 ATGCAT ATGCA^T
FbaI 699 TGATCA T^GATCA
FspI 2740 TGCGCA TGC^GCA
HhaI 2741 GCGC GCG^C
Hin1I 2600 GACGTC G[GA]^CG[CT]C
HinP1I 2741 GCGC G^CGC
HincII 522 GTCAAC GT[CT]^[GA]AC
HindII 522 GTCAAC GT[CT]^[GA]AC
HindIII 1967 AAGCTT A^AGCTT
MaeI 244 CTAG C^TAG
MvnI 911 CGCG CG^CG
NcoI 744 CCATGG C^CATGG
NsiI 815 ATGCAT ATGCA^T
NspV 668 TTCGAA TT^CGAA
PflMI 498 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3281 CACGTG CAC^GTG
PmlI 3281 CACGTG CAC^GTG
PshBI 804 ATTAAT AT^TAAT
SacI 632 GAGCTC GAGCT^C
ScaI 1793 AGTACT AGT^ACT
SfuI 668 TTCGAA TT^CGAA
SpeI 243 ACTAGT A^CTAGT
SphI 163 GCATGC GCATG^C
SspBI 2485 TGTACA T^GTACA
Van91I 498 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2320 CCATCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Alw44I 247 GTGCAC G^TGCAC
5354 GTGCAC
AocI 1366 CCTGAGG CC^T[ACGT]AGG
2290 CCTGAGG
ApaLI 247 GTGCAC G^TGCAC
5354 GTGCAC
BanII 632 GAGCTC G[GA]GC[CT]^C
2157 GAGCCC
BglI 1420 GCCGATTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2862 GCCCTACTGGC
BlpI 4023 GCTGAGC GC^T[ACGT]AGC
4666 GCTGAGC
Bpu1102I 4023 GCTGAGC GC^T[ACGT]AGC
4666 GCTGAGC
BsaI 1909 GAGACC GGTCTC(1/5)
2454 GGTCTC
BseRI 2921 CTCCTC GAGGAG(10/8)
3157 CTCCTC
BsgI 587 CTGCAC GTGCAG(16/14)
1435 GTGCAG
BsmFI 2333 GGGAC GGGAC(10/14)
2218 GTCCC
BssSI 6 CACGAG CACGAG(-5/-1)
2070 CTCGTG
Bst1107I 876 GTATAC GTA^TAC
2022 GTATAC
BstYI 1668 AGATCC [GA]^GATC[CT]
2474 GGATCC
BstZ17I 876 GTATAC GTA^TAC
2022 GTATAC
Bsu36I 1366 CCTGAGG CC^T[ACGT]AGG
2290 CCTGAGG
CelII 4023 GCTGAGC GC^T[ACGT]AGC
4666 GCTGAGC
DraI 1737 TTTAAA TTT^AAA
2892 TTTAAA
Eco81I 1366 CCTGAGG CC^T[ACGT]AGG
2290 CCTGAGG
EcoNI 1014 CCTATCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2290 CCTGAGGAAGG
MfeI 468 CAATTG C^AATTG
653 CAATTG
MflI 1668 AGATCC [GA]^GATC[CT]
2474 GGATCC
MunI 468 CAATTG C^AATTG
653 CAATTG
NdeI 68 CATATG CA^TATG
576 CATATG
PvuII 1885 CAGCTG CAG^CTG
2882 CAGCTG
StuI 114 AGGCCT AGG^CCT
1187 AGGCCT
XhoII 1668 AGATCC [GA]^GATC[CT]
2474 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BbsI BbvCI BbvI
BcgI BmyI BpmI BpuAI BsaBI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HgaI HinfI HphI ItaI
Ksp632I MaeII MaeIII MamI MboI
MboII MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I