##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI BbeI
BfrI BsiWI Bst1107I BstBI BstZ17I
DraI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FseI KasI KpnI KspI
MluI NarI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI SacII
SexAI SfoI SfuI SnaBI SpeI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
ApaI 5339 GGGCCC GGGCC^C
AviII 384 TGCGCA TGC^GCA
AvrII 7360 CCTAGG C^CTAGG
BciVI 5355 GTATCC GTATCC(6/5)
BclI 2025 TGATCA T^GATCA
BlnI 7360 CCTAGG C^CTAGG
BsiEI 3644 CGGCCG CG[GA][CT]^CG
BsmBI 7294 CGTCTC CGTCTC(1/5)
Bsp1407I 5679 TGTACA T^GTACA
BspLU11I 4935 ACATGT A^CATGT
BsrGI 5679 TGTACA T^GTACA
BssHII 5407 GCGCGC G^CGCGC
BstEII 926 GGTCACC G^GT[ACGT]ACC
BstPI 926 GGTCACC G^GT[ACGT]ACC
EagI 3644 CGGCCG C^GGCCG
EclXI 3644 CGGCCG C^GGCCG
Eco52I 3644 CGGCCG C^GGCCG
EcoO65I 926 GGTCACC G^GT[ACGT]ACC
FbaI 2025 TGATCA T^GATCA
FspI 384 TGCGCA TGC^GCA
HpaI 7952 GTTAAC GTT^AAC
PaeR7I 6992 CTCGAG C^TCGAG
PshAI 8492 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2654 GTCGAC G^TCGAC
ScaI 558 AGTACT AGT^ACT
SgrAI 4310 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 5679 TGTACA T^GTACA
SspI 8467 AATATT AAT^ATT
XhoI 6992 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2654 GTCGAC GT^[AC][GT]AC
4617 GTCTAC
AcsI 782 GAATTT [GA]^AATT[CT]
1598 AAATTC
ApoI 782 GAATTT [GA]^AATT[CT]
1598 AAATTC
AspI 653 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2219 GACCGGGTC
BamHI 4771 GGATCC G^GATCC
7575 GGATCC
BcgI 1474 CGAAGGGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1768 GCAGACAGTTCG
BglII 133 AGATCT A^GATCT
5863 AGATCT
BsaI 5931 GGTCTC GGTCTC(1/5)
6592 GGTCTC
BsmI 2185 GAATGC GAATGC(1/-1)
6109 GCATTC
BspDI 189 ATCGAT AT^CGAT
2099 ATCGAT
ClaI 189 ATCGAT AT^CGAT
2099 ATCGAT
CpoI 2217 CGGACCG CG^G[AT]CCG
5033 CGGACCG
MfeI 6415 CAATTG C^AATTG
7857 CAATTG
MunI 6415 CAATTG C^AATTG
7857 CAATTG
PflFI 653 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2219 GACCGGGTC
RsrII 2217 CGGACCG CG^G[AT]CCG
5033 CGGACCG
SacI 5137 GAGCTC GAGCT^C
8363 GAGCTC
SbfI 1939 CCTGCAGG CCTGCA^GG
7078 CCTGCAGG
Sse8387I 1939 CCTGCAGG CCTGCA^GG
7078 CCTGCAGG
Tth111I 653 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2219 GACCGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmaI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI