##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AviII BfrI BglI
BlpI Bpu1102I BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI BstBI CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI KpnI KspI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SfiI SfuI
SgrAI SnaBI SspBI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 579 CGCG CG^CG
AcyI 187 GGCGCC G[GA]^CG[CT]C
AhdI 4333 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4786 GTGCAC G^TGCAC
AocI 6228 CCTGAGG CC^T[ACGT]AGG
ApaLI 4786 GTGCAC G^TGCAC
AspEI 4333 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3695 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6107 CCTAGG C^CTAGG
BamHI 4698 GGATCC G^GATCC
BbeI 187 GGCGCC GGCGC^C
BcgI 617 GCAAGTCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 6107 CCTAGG C^CTAGG
BsaBI 4870 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 187 GGCGCC G[GA]^CG[CT]C
BstAPI 6040 GCATTGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 579 CGCG CG^CG
Bsu36I 6228 CCTGAGG CC^T[ACGT]AGG
Eam1105I 4333 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 6228 CCTGAGG CC^T[ACGT]AGG
Hin1I 187 GGCGCC G[GA]^CG[CT]C
HpaI 2120 GTTAAC GTT^AAC
KasI 187 GGCGCC G^GCGCC
MamI 4870 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 579 CGCG CG^CG
NarI 187 GGCGCC GG^CGCC
PflFI 3695 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 625 GTCGAC G^TCGAC
SbfI 4848 CCTGCAGG CCTGCA^GG
ScaI 6517 AGTACT AGT^ACT
SexAI 2510 ACCAGGT A^CC[AT]GGT
SfoI 187 GGCGCC GGC^GCC
SmaI 1682 CCCGGG CCC^GGG
SpeI 3362 ACTAGT A^CTAGT
SphI 738 GCATGC GCATG^C
Sse8387I 4848 CCTGCAGG CCTGCA^GG
Tth111I 3695 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1682 CCCGGG C^CCGGG
XmaI 1682 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 746 TCCGGA T^CCGGA
4839 TCCGGA
AflIII 4191 ACGTGT A^C[GA][CT]GT
4967 ACACGT
BbrPI 3341 CACGTG CAC^GTG
4968 CACGTG
BbvCI 4208 CCTCAGC CCTCAGC(-5/-2)
3457 GCTGAGG
BciVI 5797 GGATAC GTATCC(6/5)
5955 GTATCC
BsaAI 3341 CACGTG [CT]AC^GT[GA]
4968 CACGTG
BsaWI 746 TCCGGA [AT]^CCGG[AT]
4839 TCCGGA
BseAI 746 TCCGGA T^CCGGA
4839 TCCGGA
BsmBI 2024 CGTCTC CGTCTC(1/5)
1795 GAGACG
BspEI 746 TCCGGA T^CCGGA
4839 TCCGGA
BsrDI 437 CATTGC GCAATG(2/0)
2526 CATTGC
Bst1107I 3321 GTATAC GTA^TAC
5880 GTATAC
BstEII 918 GGTAACC G^GT[ACGT]ACC
2934 GGTTACC
BstPI 918 GGTAACC G^GT[ACGT]ACC
2934 GGTTACC
BstZ17I 3321 GTATAC GTA^TAC
5880 GTATAC
EcoO65I 918 GGTAACC G^GT[ACGT]ACC
2934 GGTTACC
EcoRI 967 GAATTC G^AATTC
4770 GAATTC
MroI 746 TCCGGA T^CCGGA
4839 TCCGGA
PflMI 2868 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3762 CCAGCCTGTGG
PmaCI 3341 CACGTG CAC^GTG
4968 CACGTG
PmlI 3341 CACGTG CAC^GTG
4968 CACGTG
SacI 1183 GAGCTC GAGCT^C
4835 GAGCTC
SapI 5643 GAAGAGC GCTCTTC(1/4)
6270 GAAGAGC
SspI 3653 AATATT AAT^ATT
4610 AATATT
Van91I 2868 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3762 CCAGCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PaeR7I PleI PpuMI PshAI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XcmI XhoI XhoII