##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     Asp718I     AviII       BfrI        BglI        
BlpI        Bpu1102I    BsiEI       BsiWI       Bsp1407I    
BspDI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       BstBI       CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        EagI        
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FseI        FspI        KpnI        KspI        MfeI        
MluI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SfiI        SfuI        
SgrAI       SnaBI       SspBI       SwaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccII         579  CGCG                 CG^CG
AcyI          187  GGCGCC               G[GA]^CG[CT]C
AhdI         4333  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       4786  GTGCAC               G^TGCAC
AocI         6228  CCTGAGG              CC^T[ACGT]AGG
ApaLI        4786  GTGCAC               G^TGCAC
AspEI        4333  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         3695  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        6107  CCTAGG               C^CTAGG
BamHI        4698  GGATCC               G^GATCC
BbeI          187  GGCGCC               GGCGC^C
BcgI          617  GCAAGTCTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         6107  CCTAGG               C^CTAGG
BsaBI        4870  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         187  GGCGCC               G[GA]^CG[CT]C
BstAPI       6040  GCATTGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         579  CGCG                 CG^CG
Bsu36I       6228  CCTGAGG              CC^T[ACGT]AGG
Eam1105I     4333  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       6228  CCTGAGG              CC^T[ACGT]AGG
Hin1I         187  GGCGCC               G[GA]^CG[CT]C
HpaI         2120  GTTAAC               GTT^AAC
KasI          187  GGCGCC               G^GCGCC
MamI         4870  GATCTGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI          579  CGCG                 CG^CG
NarI          187  GGCGCC               GG^CGCC
PflFI        3695  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SalI          625  GTCGAC               G^TCGAC
SbfI         4848  CCTGCAGG             CCTGCA^GG
ScaI         6517  AGTACT               AGT^ACT
SexAI        2510  ACCAGGT              A^CC[AT]GGT
SfoI          187  GGCGCC               GGC^GCC
SmaI         1682  CCCGGG               CCC^GGG
SpeI         3362  ACTAGT               A^CTAGT
SphI          738  GCATGC               GCATG^C
Sse8387I     4848  CCTGCAGG             CCTGCA^GG
Tth111I      3695  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1682  CCCGGG               C^CCGGG
XmaI         1682  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccIII        746  TCCGGA               T^CCGGA
             4839  TCCGGA              
AflIII       4191  ACGTGT               A^C[GA][CT]GT
             4967  ACACGT              
BbrPI        3341  CACGTG               CAC^GTG
             4968  CACGTG              
BbvCI        4208  CCTCAGC              CCTCAGC(-5/-2)
             3457  GCTGAGG             
BciVI        5797  GGATAC               GTATCC(6/5)
             5955  GTATCC              
BsaAI        3341  CACGTG               [CT]AC^GT[GA]
             4968  CACGTG              
BsaWI         746  TCCGGA               [AT]^CCGG[AT]
             4839  TCCGGA              
BseAI         746  TCCGGA               T^CCGGA
             4839  TCCGGA              
BsmBI        2024  CGTCTC               CGTCTC(1/5)
             1795  GAGACG              
BspEI         746  TCCGGA               T^CCGGA
             4839  TCCGGA              
BsrDI         437  CATTGC               GCAATG(2/0)
             2526  CATTGC              
Bst1107I     3321  GTATAC               GTA^TAC
             5880  GTATAC              
BstEII        918  GGTAACC              G^GT[ACGT]ACC
             2934  GGTTACC             
BstPI         918  GGTAACC              G^GT[ACGT]ACC
             2934  GGTTACC             
BstZ17I      3321  GTATAC               GTA^TAC
             5880  GTATAC              
EcoO65I       918  GGTAACC              G^GT[ACGT]ACC
             2934  GGTTACC             
EcoRI         967  GAATTC               G^AATTC
             4770  GAATTC              
MroI          746  TCCGGA               T^CCGGA
             4839  TCCGGA              
PflMI        2868  CCACAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3762  CCAGCCTGTGG         
PmaCI        3341  CACGTG               CAC^GTG
             4968  CACGTG              
PmlI         3341  CACGTG               CAC^GTG
             4968  CACGTG              
SacI         1183  GAGCTC               GAGCT^C
             4835  GAGCTC              
SapI         5643  GAAGAGC              GCTCTTC(1/4)
             6270  GAAGAGC             
SspI         3653  AATATT               AAT^ATT
             4610  AATATT              
Van91I       2868  CCACAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3762  CCAGCCTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BclI        BcnI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BspHI       BspMI       BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PaeR7I      PleI        PpuMI       PshAI       
PstI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XbaI        XcmI        XhoI        XhoII