##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AgeI        AocI        Aor51HI     AscI        
Asp718I     AviII       BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BsaAI       BsaHI       
BsiEI       BsiWI       BsmBI       BspDI       BsrBI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      ClaI        CpoI        DrdI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoRV       FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        MfeI        MluI        MunI        
NarI        NcoI        NotI        NruI        NspV        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RsrII       SacI        
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       Sse8387I    
StuI        SwaI        Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII          16  CGCG                 CG^CG
AccIII         98  TCCGGA               T^CCGGA
AflIII       1367  ACATGT               A^C[GA][CT]GT
AhdI          753  GACATGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        768  GTGCAC               G^TGCAC
AlwNI        1435  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         768  GTGCAC               G^TGCAC
Asp700I      1156  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         753  GACATGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1094  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        2676  GGATCC               G^GATCC
BanI         4044  GGCACC               G^G[CT][GA]CC
BbvCI        4010  GCTGAGG              CCTCAGC(-5/-2)
BlpI         1107  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     1107  GCTTAGC              GC^T[ACGT]AGC
BsaWI          98  TCCGGA               [AT]^CCGG[AT]
BseAI          98  TCCGGA               T^CCGGA
BspEI          98  TCCGGA               T^CCGGA
BspLU11I     1367  ACATGT               A^CATGT
BsrDI        3932  GCAATG               GCAATG(2/0)
BstAPI        508  GCAGAAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1536  GGTGACC              G^GT[ACGT]ACC
BstPI        1536  GGTGACC              G^GT[ACGT]ACC
BstUI          16  CGCG                 CG^CG
BstXI        1999  CCAGATTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1107  GCTTAGC              GC^T[ACGT]AGC
Eam1105I      753  GACATGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1536  GGTGACC              G^GT[ACGT]ACC
EcoT22I       404  ATGCAT               ATGCA^T
HaeII        1751  GGCGCT               [GA]GCGC^[CT]
HindIII      2490  AAGCTT               A^AGCTT
KspI           15  CCGCGG               CCGC^GG
MroI           98  TCCGGA               T^CCGGA
MvnI           16  CGCG                 CG^CG
NsiI          404  ATGCAT               ATGCA^T
PacI         4225  TTAATTAA             TTAAT^TAA
PflFI        1094  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacII          15  CCGCGG               CCGC^GG
SapI         2993  GCTCTTC              GCTCTTC(1/4)
SmaI           39  CCCGGG               CCC^GGG
SpeI         3741  ACTAGT               A^CTAGT
SphI         2840  GCATGC               GCATG^C
SspI         3206  AATATT               AAT^ATT
TaqI         2789  TCGA                 T^CGA
Tth111I      1094  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      2789  TCGA                 T^CGA
XbaI         2729  TCTAGA               T^CTAGA
XcmI         1450  CCAAGCTAGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          39  CCCGGG               C^CCGGG
XmaI           39  CCCGGG               C^CCGGG
XmnI         1156  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

ApaI           36  GGGCCC               GGGCC^C
              142  GGGCCC              
AvaI           39  CCCGGG               C^[CT]CG[GA]G
              698  CCCGAG              
AvrII        1176  CCTAGG               C^CTAGG
             2427  CCTAGG              
BalI         2771  TGGCCA               TGG^CCA
             3891  TGGCCA              
BglI           14  GCCGCGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3536  GCCTTCATGGC         
BglII         925  AGATCT               A^GATCT
             4220  AGATCT              
BlnI         1176  CCTAGG               C^CTAGG
             2427  CCTAGG              
BsaBI         866  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2375  GATGTAAATC          
BsmI         2814  GAATGC               GAATGC(1/-1)
             1587  GCATTC              
BsoBI          39  CCCGGG               C^[CT]CG[GA]G
              698  CCCGAG              
Bsp1407I      264  TGTACA               T^GTACA
             2507  TGTACA              
BsrFI          30  GCCGGC               [GA]^CCGG[CT]
             3911  GCCGGC              
BsrGI         264  TGTACA               T^GTACA
             2507  TGTACA              
CfoI         1752  GCGC                 GCG^C
             3179  GCGC                
Cfr10I         30  GCCGGC               [GA]^CCGG[CT]
             3911  GCCGGC              
DsaI           15  CCGCGG               C^C[GA][CT]GG
              692  CCACGG              
EaeI         2771  TGGCCA               [CT]^GGCC[GA]
             3891  TGGCCA              
HhaI         1752  GCGC                 GCG^C
             3179  GCGC                
HinP1I       1752  GCGC                 G^CGC
             3179  GCGC                
MaeII         687  ACGT                 A^CGT
             1295  ACGT                
MamI          866  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2375  GATGTAAATC          
MluNI        2771  TGGCCA               TGG^CCA
             3891  TGGCCA              
MscI         2771  TGGCCA               TGG^CCA
             3891  TGGCCA              
NaeI           30  GCCGGC               GCC^GGC
             3911  GCCGGC              
NdeI         1258  CATATG               CA^TATG
             4188  CATATG              
NgoMIV         30  GCCGGC               G^CCGGC
             3911  GCCGGC              
NheI         1324  GCTAGC               G^CTAGC
             1454  GCTAGC              
PpuMI        1244  AGGACCT              [GA]G^G[AT]CC[CT]
             1486  AGGACCT             
PshAI        1439  GACTGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3476  GACAGGAGTC          
PvuII        3004  CAGCTG               CAG^CTG
             4096  CAGCTG              
SspBI         264  TGTACA               T^GTACA
             2507  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        AspHI       AvaII       
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspHI       BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DraII       DraIII      EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PshBI       
PstI        RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII